Touch-stimulation increases host-seeking behavior in Steinernema Carpocapsae
Previous research demonstrated that Steinernema carpocapsae infective juveniles (IJs) exposed to a host cuticle were more attracted toward certain host-associated volatile odors. We wanted to test the specificity of attraction that results from exposure to host cuticle. Host recognition behavior was...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2019-01-01
|
| Series: | Journal of Nematology |
| Subjects: | |
| Online Access: | https://doi.org/10.21307/jofnem-2019-067 |
| _version_ | 1811159238819446784 |
|---|---|
| author | Baiocchi Tiffany Braun Lauren Dillman Adler R. |
| author_facet | Baiocchi Tiffany Braun Lauren Dillman Adler R. |
| author_sort | Baiocchi Tiffany |
| collection | DOAJ |
| description | Previous research demonstrated that Steinernema carpocapsae infective juveniles (IJs) exposed to a host cuticle were more attracted toward certain host-associated volatile odors. We wanted to test the specificity of attraction that results from exposure to host cuticle. Host recognition behavior was analyzed after stimulating IJs by allowing them to physically interact with Galleria mellonella cuticles. The subsequent behavioral response and the proportion of the population participating in chemotaxis to multiple host odors were measured. We found that exposure to host cuticles resulted in a significantly higher percentage of the population participating in host-seeking behavior, with threefold more nematodes participating in chemotaxis. We tested whether exposure to live or dead host cuticle resulted in a different response and found that a higher percentage of IJs exposed to a live host cuticle participated in chemotaxis than IJs exposed to a dead host cuticle, but that IJs exposed to a dead host demonstrated significantly higher participation than was observed for non-stimulated IJs. To test whether the increase in IJ participation in host-seeking behaviors after exposure to a live host cuticle was specific, we exposed stimulated IJs to a known repulsive odor, a neutral odor, and two predicted attractants. We found that stimulation of IJs through physical contact with a host cuticle induces a specific enhancement of host-seeking behavior to host-specific odors rather than a general increased chemotactic response to all volatile stimuli. However, the nematodes displayed an enhanced response to multiple host-specific odors. Future work should focus on the mechanism through which contact with live host cuticle stimulates increased behavioral response. |
| first_indexed | 2024-04-10T05:37:44Z |
| format | Article |
| id | doaj.art-54679b0b54ce4a69ab77f22dee9907fb |
| institution | Directory Open Access Journal |
| issn | 2640-396X |
| language | English |
| last_indexed | 2024-04-10T05:37:44Z |
| publishDate | 2019-01-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Nematology |
| spelling | doaj.art-54679b0b54ce4a69ab77f22dee9907fb2023-03-06T16:52:20ZengSciendoJournal of Nematology2640-396X2019-01-015111510.21307/jofnem-2019-067Touch-stimulation increases host-seeking behavior in Steinernema CarpocapsaeBaiocchi Tiffany0Braun Lauren1Dillman Adler R.2Department of Nematology, University of California Riverside, Riverside, CA, 92521USA Department of Nematology, University of California Riverside, Riverside, CA, 92521USA Department of Nematology, University of California Riverside, Riverside, CA, 92521USA Previous research demonstrated that Steinernema carpocapsae infective juveniles (IJs) exposed to a host cuticle were more attracted toward certain host-associated volatile odors. We wanted to test the specificity of attraction that results from exposure to host cuticle. Host recognition behavior was analyzed after stimulating IJs by allowing them to physically interact with Galleria mellonella cuticles. The subsequent behavioral response and the proportion of the population participating in chemotaxis to multiple host odors were measured. We found that exposure to host cuticles resulted in a significantly higher percentage of the population participating in host-seeking behavior, with threefold more nematodes participating in chemotaxis. We tested whether exposure to live or dead host cuticle resulted in a different response and found that a higher percentage of IJs exposed to a live host cuticle participated in chemotaxis than IJs exposed to a dead host cuticle, but that IJs exposed to a dead host demonstrated significantly higher participation than was observed for non-stimulated IJs. To test whether the increase in IJ participation in host-seeking behaviors after exposure to a live host cuticle was specific, we exposed stimulated IJs to a known repulsive odor, a neutral odor, and two predicted attractants. We found that stimulation of IJs through physical contact with a host cuticle induces a specific enhancement of host-seeking behavior to host-specific odors rather than a general increased chemotactic response to all volatile stimuli. However, the nematodes displayed an enhanced response to multiple host-specific odors. Future work should focus on the mechanism through which contact with live host cuticle stimulates increased behavioral response.https://doi.org/10.21307/jofnem-2019-067steinernemahost-seeking behaviorchemotaxis |
| spellingShingle | Baiocchi Tiffany Braun Lauren Dillman Adler R. Touch-stimulation increases host-seeking behavior in Steinernema Carpocapsae Journal of Nematology steinernema host-seeking behavior chemotaxis |
| title | Touch-stimulation increases host-seeking behavior in Steinernema Carpocapsae |
| title_full | Touch-stimulation increases host-seeking behavior in Steinernema Carpocapsae |
| title_fullStr | Touch-stimulation increases host-seeking behavior in Steinernema Carpocapsae |
| title_full_unstemmed | Touch-stimulation increases host-seeking behavior in Steinernema Carpocapsae |
| title_short | Touch-stimulation increases host-seeking behavior in Steinernema Carpocapsae |
| title_sort | touch stimulation increases host seeking behavior in steinernema carpocapsae |
| topic | steinernema host-seeking behavior chemotaxis |
| url | https://doi.org/10.21307/jofnem-2019-067 |
| work_keys_str_mv | AT baiocchitiffany touchstimulationincreaseshostseekingbehaviorinsteinernemacarpocapsae AT braunlauren touchstimulationincreaseshostseekingbehaviorinsteinernemacarpocapsae AT dillmanadlerr touchstimulationincreaseshostseekingbehaviorinsteinernemacarpocapsae |