Preparation of Fucoidan-Based Electrospun Nanofibers and Their Interaction With Endothelial Cells
Sulfated polysaccharide fucoidan (FD) is widely applied in biomedical applications owing to its outstanding bioactivities. In addition to the biochemical features, the architecture of biomaterials plays a critical role in tissue repair and regeneration. Particularly, nanofibers have elicited great i...
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.739209/full |
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| author | Yiwen Chen Yiwen Chen Huilin Zhu Huilin Zhu Yuanping Hao Zhanyi Sun Peili Shen Qihui Zhou Qihui Zhou |
| author_facet | Yiwen Chen Yiwen Chen Huilin Zhu Huilin Zhu Yuanping Hao Zhanyi Sun Peili Shen Qihui Zhou Qihui Zhou |
| author_sort | Yiwen Chen |
| collection | DOAJ |
| description | Sulfated polysaccharide fucoidan (FD) is widely applied in biomedical applications owing to its outstanding bioactivities. In addition to the biochemical features, the architecture of biomaterials plays a critical role in tissue repair and regeneration. Particularly, nanofibers have elicited great interest due to their extracellular matrix-like structure, high specific surface area, and favorable biological properties. Herein, chitosan-modified FD/ultra-high molecular weight polyethylene oxide (UHMWPEO) nanofibers are developed via green electrospinning and electrostatic interaction for studying their interaction with endothelial cells. The appropriate solvent is screened to dissolve FD. The electrospinnability of FD/UHMWPEO aqueous solutions is greatly dependent on the weight ratios of FD/UHMWPEO. The incorporation of UHMWPEO significantly improves the electrospinnability of solution and thermo-stability of nanofibers. Also, it is found that there is good miscibility or no phase separation in FD/UHMWPEO solutions. In vitro biological experiments show that the chitosan-modified FD/UHMWPEO nanofibers greatly facilitate the adhesion of endothelial cells and inhibit the attachment of monocytes. Thus, the designed FD-based nanofibers are promising bio-scaffolds in building tissue-engineered blood vessels. |
| first_indexed | 2024-12-16T12:22:20Z |
| format | Article |
| id | doaj.art-546b6f0053684611b5eddbc7fd2f599d |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-16T12:22:20Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-546b6f0053684611b5eddbc7fd2f599d2022-12-21T22:31:56ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-09-01910.3389/fbioe.2021.739209739209Preparation of Fucoidan-Based Electrospun Nanofibers and Their Interaction With Endothelial CellsYiwen Chen0Yiwen Chen1Huilin Zhu2Huilin Zhu3Yuanping Hao4Zhanyi Sun5Peili Shen6Qihui Zhou7Qihui Zhou8Department of Stomatology, Institute for Translational Medicine, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, ChinaSchool of Stomatology, Qingdao University, Qingdao, ChinaDepartment of Stomatology, Institute for Translational Medicine, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, ChinaSchool of Stomatology, Qingdao University, Qingdao, ChinaDepartment of Stomatology, Institute for Translational Medicine, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, ChinaState Key Laboratory of Bioactive Seaweed Substances, Qingdao Bright Moon Seaweed Group Co., Ltd., Qingdao, ChinaState Key Laboratory of Bioactive Seaweed Substances, Qingdao Bright Moon Seaweed Group Co., Ltd., Qingdao, ChinaDepartment of Stomatology, Institute for Translational Medicine, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, ChinaSchool of Stomatology, Qingdao University, Qingdao, ChinaSulfated polysaccharide fucoidan (FD) is widely applied in biomedical applications owing to its outstanding bioactivities. In addition to the biochemical features, the architecture of biomaterials plays a critical role in tissue repair and regeneration. Particularly, nanofibers have elicited great interest due to their extracellular matrix-like structure, high specific surface area, and favorable biological properties. Herein, chitosan-modified FD/ultra-high molecular weight polyethylene oxide (UHMWPEO) nanofibers are developed via green electrospinning and electrostatic interaction for studying their interaction with endothelial cells. The appropriate solvent is screened to dissolve FD. The electrospinnability of FD/UHMWPEO aqueous solutions is greatly dependent on the weight ratios of FD/UHMWPEO. The incorporation of UHMWPEO significantly improves the electrospinnability of solution and thermo-stability of nanofibers. Also, it is found that there is good miscibility or no phase separation in FD/UHMWPEO solutions. In vitro biological experiments show that the chitosan-modified FD/UHMWPEO nanofibers greatly facilitate the adhesion of endothelial cells and inhibit the attachment of monocytes. Thus, the designed FD-based nanofibers are promising bio-scaffolds in building tissue-engineered blood vessels.https://www.frontiersin.org/articles/10.3389/fbioe.2021.739209/fullfucoidanelectrospun nanofibersextracellular matrixendothelial cellsbiointerfacecell-material interface |
| spellingShingle | Yiwen Chen Yiwen Chen Huilin Zhu Huilin Zhu Yuanping Hao Zhanyi Sun Peili Shen Qihui Zhou Qihui Zhou Preparation of Fucoidan-Based Electrospun Nanofibers and Their Interaction With Endothelial Cells Frontiers in Bioengineering and Biotechnology fucoidan electrospun nanofibers extracellular matrix endothelial cells biointerface cell-material interface |
| title | Preparation of Fucoidan-Based Electrospun Nanofibers and Their Interaction With Endothelial Cells |
| title_full | Preparation of Fucoidan-Based Electrospun Nanofibers and Their Interaction With Endothelial Cells |
| title_fullStr | Preparation of Fucoidan-Based Electrospun Nanofibers and Their Interaction With Endothelial Cells |
| title_full_unstemmed | Preparation of Fucoidan-Based Electrospun Nanofibers and Their Interaction With Endothelial Cells |
| title_short | Preparation of Fucoidan-Based Electrospun Nanofibers and Their Interaction With Endothelial Cells |
| title_sort | preparation of fucoidan based electrospun nanofibers and their interaction with endothelial cells |
| topic | fucoidan electrospun nanofibers extracellular matrix endothelial cells biointerface cell-material interface |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.739209/full |
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