A Copper (II) Ensemble-Based Fluorescence Chemosensor and Its Application in the ‘Naked–Eye’ Detection of Biothiols in Human Urine
Quick and effective detection of biothiols in biological fluids has gained increasing attention due to its vital biological functions. In this paper, a novel reversible fluorescence chemosensor (<b>L</b>-Cu<sup>2+</sup>) based on a benzocoumarin-Cu<sup>2+</sup> en...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-02-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/20/5/1331 |
| _version_ | 1798041787268333568 |
|---|---|
| author | Yue Wang Huan Feng Haibo Li Xinyi Yang Hongmin Jia Wenjun Kang Qingtao Meng Zhiqiang Zhang Run Zhang |
| author_facet | Yue Wang Huan Feng Haibo Li Xinyi Yang Hongmin Jia Wenjun Kang Qingtao Meng Zhiqiang Zhang Run Zhang |
| author_sort | Yue Wang |
| collection | DOAJ |
| description | Quick and effective detection of biothiols in biological fluids has gained increasing attention due to its vital biological functions. In this paper, a novel reversible fluorescence chemosensor (<b>L</b>-Cu<sup>2+</sup>) based on a benzocoumarin-Cu<sup>2+</sup> ensemble has been developed for the detection of biothiols (Cys, Hcy and GSH) in human urine. The chemosensing ensemble (<b>L</b>-Cu<sup>2+</sup>) contains a 2:1 stoichiometry structure between fluorescent ligand <b>L</b> and paramagnetic Cu<sup>2+</sup>. <b>L</b> was found to exclusively bond with Cu<sup>2+</sup> ions accompanied with a dramatic fluorescence quenching maximum at 443 nm and an increase of an absorbance band centered at 378 nm. Then, the in situ generated fluorescence sluggish ensemble, <b>L</b>-Cu<sup>2+</sup>, was successfully used as a chemosensor for the detection of biothiols with a fluorescence “OFF-ON” response modality. Upon the addition of biothiols, the decomplexation of <b>L</b>-Cu<sup>2+</sup> led to the liberation of the fluorescent ligand, <b>L</b>, resulting in the recovery of fluorescence and absorbance spectra. Studies revealed that <b>L</b>-Cu<sup>2+</sup> possesses simple synthesis, excellent stability, high sensitivity, reliability at a broad pH range and desired renewability (at least 5 times). The practical application of <b>L</b>-Cu<sup>2+</sup> was then demonstrated by the detection of biothiols in human urine sample. |
| first_indexed | 2024-04-11T22:26:30Z |
| format | Article |
| id | doaj.art-546f9b20feae4dc6ac63f3005cd6ae73 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T22:26:30Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-546f9b20feae4dc6ac63f3005cd6ae732022-12-22T03:59:39ZengMDPI AGSensors1424-82202020-02-01205133110.3390/s20051331s20051331A Copper (II) Ensemble-Based Fluorescence Chemosensor and Its Application in the ‘Naked–Eye’ Detection of Biothiols in Human UrineYue Wang0Huan Feng1Haibo Li2Xinyi Yang3Hongmin Jia4Wenjun Kang5Qingtao Meng6Zhiqiang Zhang7Run Zhang8School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, ChinaSchool of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, ChinaShandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, Department of Chemistry, Liaocheng University, Liaocheng 252059, ChinaSchool of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, ChinaSchool of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, ChinaShandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, Department of Chemistry, Liaocheng University, Liaocheng 252059, ChinaSchool of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, ChinaSchool of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, ChinaAustralian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane 4072, AustraliaQuick and effective detection of biothiols in biological fluids has gained increasing attention due to its vital biological functions. In this paper, a novel reversible fluorescence chemosensor (<b>L</b>-Cu<sup>2+</sup>) based on a benzocoumarin-Cu<sup>2+</sup> ensemble has been developed for the detection of biothiols (Cys, Hcy and GSH) in human urine. The chemosensing ensemble (<b>L</b>-Cu<sup>2+</sup>) contains a 2:1 stoichiometry structure between fluorescent ligand <b>L</b> and paramagnetic Cu<sup>2+</sup>. <b>L</b> was found to exclusively bond with Cu<sup>2+</sup> ions accompanied with a dramatic fluorescence quenching maximum at 443 nm and an increase of an absorbance band centered at 378 nm. Then, the in situ generated fluorescence sluggish ensemble, <b>L</b>-Cu<sup>2+</sup>, was successfully used as a chemosensor for the detection of biothiols with a fluorescence “OFF-ON” response modality. Upon the addition of biothiols, the decomplexation of <b>L</b>-Cu<sup>2+</sup> led to the liberation of the fluorescent ligand, <b>L</b>, resulting in the recovery of fluorescence and absorbance spectra. Studies revealed that <b>L</b>-Cu<sup>2+</sup> possesses simple synthesis, excellent stability, high sensitivity, reliability at a broad pH range and desired renewability (at least 5 times). The practical application of <b>L</b>-Cu<sup>2+</sup> was then demonstrated by the detection of biothiols in human urine sample.https://www.mdpi.com/1424-8220/20/5/1331chemosensorbiothiolsdisplacementdetectionurine samples |
| spellingShingle | Yue Wang Huan Feng Haibo Li Xinyi Yang Hongmin Jia Wenjun Kang Qingtao Meng Zhiqiang Zhang Run Zhang A Copper (II) Ensemble-Based Fluorescence Chemosensor and Its Application in the ‘Naked–Eye’ Detection of Biothiols in Human Urine Sensors chemosensor biothiols displacement detection urine samples |
| title | A Copper (II) Ensemble-Based Fluorescence Chemosensor and Its Application in the ‘Naked–Eye’ Detection of Biothiols in Human Urine |
| title_full | A Copper (II) Ensemble-Based Fluorescence Chemosensor and Its Application in the ‘Naked–Eye’ Detection of Biothiols in Human Urine |
| title_fullStr | A Copper (II) Ensemble-Based Fluorescence Chemosensor and Its Application in the ‘Naked–Eye’ Detection of Biothiols in Human Urine |
| title_full_unstemmed | A Copper (II) Ensemble-Based Fluorescence Chemosensor and Its Application in the ‘Naked–Eye’ Detection of Biothiols in Human Urine |
| title_short | A Copper (II) Ensemble-Based Fluorescence Chemosensor and Its Application in the ‘Naked–Eye’ Detection of Biothiols in Human Urine |
| title_sort | copper ii ensemble based fluorescence chemosensor and its application in the naked eye detection of biothiols in human urine |
| topic | chemosensor biothiols displacement detection urine samples |
| url | https://www.mdpi.com/1424-8220/20/5/1331 |
| work_keys_str_mv | AT yuewang acopperiiensemblebasedfluorescencechemosensoranditsapplicationinthenakedeyedetectionofbiothiolsinhumanurine AT huanfeng acopperiiensemblebasedfluorescencechemosensoranditsapplicationinthenakedeyedetectionofbiothiolsinhumanurine AT haiboli acopperiiensemblebasedfluorescencechemosensoranditsapplicationinthenakedeyedetectionofbiothiolsinhumanurine AT xinyiyang acopperiiensemblebasedfluorescencechemosensoranditsapplicationinthenakedeyedetectionofbiothiolsinhumanurine AT hongminjia acopperiiensemblebasedfluorescencechemosensoranditsapplicationinthenakedeyedetectionofbiothiolsinhumanurine AT wenjunkang acopperiiensemblebasedfluorescencechemosensoranditsapplicationinthenakedeyedetectionofbiothiolsinhumanurine AT qingtaomeng acopperiiensemblebasedfluorescencechemosensoranditsapplicationinthenakedeyedetectionofbiothiolsinhumanurine AT zhiqiangzhang acopperiiensemblebasedfluorescencechemosensoranditsapplicationinthenakedeyedetectionofbiothiolsinhumanurine AT runzhang acopperiiensemblebasedfluorescencechemosensoranditsapplicationinthenakedeyedetectionofbiothiolsinhumanurine AT yuewang copperiiensemblebasedfluorescencechemosensoranditsapplicationinthenakedeyedetectionofbiothiolsinhumanurine AT huanfeng copperiiensemblebasedfluorescencechemosensoranditsapplicationinthenakedeyedetectionofbiothiolsinhumanurine AT haiboli copperiiensemblebasedfluorescencechemosensoranditsapplicationinthenakedeyedetectionofbiothiolsinhumanurine AT xinyiyang copperiiensemblebasedfluorescencechemosensoranditsapplicationinthenakedeyedetectionofbiothiolsinhumanurine AT hongminjia copperiiensemblebasedfluorescencechemosensoranditsapplicationinthenakedeyedetectionofbiothiolsinhumanurine AT wenjunkang copperiiensemblebasedfluorescencechemosensoranditsapplicationinthenakedeyedetectionofbiothiolsinhumanurine AT qingtaomeng copperiiensemblebasedfluorescencechemosensoranditsapplicationinthenakedeyedetectionofbiothiolsinhumanurine AT zhiqiangzhang copperiiensemblebasedfluorescencechemosensoranditsapplicationinthenakedeyedetectionofbiothiolsinhumanurine AT runzhang copperiiensemblebasedfluorescencechemosensoranditsapplicationinthenakedeyedetectionofbiothiolsinhumanurine |