Cytotoxic Potential of the Marine Diatom <em>Thalassiosira rotula</em>: Insights into Bioactivity of 24-Methylene Cholesterol
Marine microalgae are receiving great interest as sustainable sources of bioactive metabolites for health, nutrition and personal care. In the present study, a bioassay-guided screening allowed identifying an enriched fraction from SPE separation of the methanolic extract of the marine diatom <i&...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/10/595 |
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| author | Adele Cutignano Mariarosaria Conte Virginia Tirino Vitale Del Vecchio Roberto De Angelis Angela Nebbioso Lucia Altucci Giovanna Romano |
| author_facet | Adele Cutignano Mariarosaria Conte Virginia Tirino Vitale Del Vecchio Roberto De Angelis Angela Nebbioso Lucia Altucci Giovanna Romano |
| author_sort | Adele Cutignano |
| collection | DOAJ |
| description | Marine microalgae are receiving great interest as sustainable sources of bioactive metabolites for health, nutrition and personal care. In the present study, a bioassay-guided screening allowed identifying an enriched fraction from SPE separation of the methanolic extract of the marine diatom <i>Thalassiosira rotula</i> with a chemically heterogeneous composition of cytotoxic molecules, including PUFAs, the terpene phytol, the carotenoid fucoxanthin and the phytosterol 24-methylene cholesterol (24-MChol). In particular, this latter was the object of deep investigation aimed to gain insight into the mechanisms of action activated in two tumour cell models recognised as resistant to chemical treatments, the breast MCF7 and the lung A549 cell lines. The results of our studies revealed that 24-MChol, in line with the most studied β-sitosterol (β-SIT), showed cytotoxic activity in a 3–30 µM range of concentration involving the induction of apoptosis and cell cycle arrest, although differences emerged between the two sterols and the two cancer systems when specific targets were investigated (caspase-3, caspase-9, FAS and TRAIL). |
| first_indexed | 2024-03-09T12:11:43Z |
| format | Article |
| id | doaj.art-546fef087bc04982bf15608a78582738 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T12:11:43Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-546fef087bc04982bf15608a785827382023-11-30T22:51:05ZengMDPI AGMarine Drugs1660-33972022-09-01201059510.3390/md20100595Cytotoxic Potential of the Marine Diatom <em>Thalassiosira rotula</em>: Insights into Bioactivity of 24-Methylene CholesterolAdele Cutignano0Mariarosaria Conte1Virginia Tirino2Vitale Del Vecchio3Roberto De Angelis4Angela Nebbioso5Lucia Altucci6Giovanna Romano7Institute of Biomolecular Chemistry, National Research Council, Via Campi Flegrei 34, 80078 Pozzuoli, ItalyDepartment of Precision Medicine, University of Campania ‘L. Vanvitelli’, Via L. De Crecchio 7, 80138 Napoli, ItalyDepartment of Experimental Medicine, Section of Biotechnology, Molecular Medicine and Medical Histology, University of Campania “L. Vanvitelli”, Via L. de Crecchio 7, 80138 Napoli, ItalyDepartment of Experimental Medicine, Section of Biotechnology, Molecular Medicine and Medical Histology, University of Campania “L. Vanvitelli”, Via L. de Crecchio 7, 80138 Napoli, ItalyDepartment of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Via Akton 55, 80133 Napoli, ItalyDepartment of Precision Medicine, University of Campania ‘L. Vanvitelli’, Via L. De Crecchio 7, 80138 Napoli, ItalyDepartment of Precision Medicine, University of Campania ‘L. Vanvitelli’, Via L. De Crecchio 7, 80138 Napoli, ItalyDepartment of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Via Akton 55, 80133 Napoli, ItalyMarine microalgae are receiving great interest as sustainable sources of bioactive metabolites for health, nutrition and personal care. In the present study, a bioassay-guided screening allowed identifying an enriched fraction from SPE separation of the methanolic extract of the marine diatom <i>Thalassiosira rotula</i> with a chemically heterogeneous composition of cytotoxic molecules, including PUFAs, the terpene phytol, the carotenoid fucoxanthin and the phytosterol 24-methylene cholesterol (24-MChol). In particular, this latter was the object of deep investigation aimed to gain insight into the mechanisms of action activated in two tumour cell models recognised as resistant to chemical treatments, the breast MCF7 and the lung A549 cell lines. The results of our studies revealed that 24-MChol, in line with the most studied β-sitosterol (β-SIT), showed cytotoxic activity in a 3–30 µM range of concentration involving the induction of apoptosis and cell cycle arrest, although differences emerged between the two sterols and the two cancer systems when specific targets were investigated (caspase-3, caspase-9, FAS and TRAIL).https://www.mdpi.com/1660-3397/20/10/595diatoms<i>Thalassiosira rotula</i>phytosterols24-methylene cholesterolcytotoxicitycancer |
| spellingShingle | Adele Cutignano Mariarosaria Conte Virginia Tirino Vitale Del Vecchio Roberto De Angelis Angela Nebbioso Lucia Altucci Giovanna Romano Cytotoxic Potential of the Marine Diatom <em>Thalassiosira rotula</em>: Insights into Bioactivity of 24-Methylene Cholesterol Marine Drugs diatoms <i>Thalassiosira rotula</i> phytosterols 24-methylene cholesterol cytotoxicity cancer |
| title | Cytotoxic Potential of the Marine Diatom <em>Thalassiosira rotula</em>: Insights into Bioactivity of 24-Methylene Cholesterol |
| title_full | Cytotoxic Potential of the Marine Diatom <em>Thalassiosira rotula</em>: Insights into Bioactivity of 24-Methylene Cholesterol |
| title_fullStr | Cytotoxic Potential of the Marine Diatom <em>Thalassiosira rotula</em>: Insights into Bioactivity of 24-Methylene Cholesterol |
| title_full_unstemmed | Cytotoxic Potential of the Marine Diatom <em>Thalassiosira rotula</em>: Insights into Bioactivity of 24-Methylene Cholesterol |
| title_short | Cytotoxic Potential of the Marine Diatom <em>Thalassiosira rotula</em>: Insights into Bioactivity of 24-Methylene Cholesterol |
| title_sort | cytotoxic potential of the marine diatom em thalassiosira rotula em insights into bioactivity of 24 methylene cholesterol |
| topic | diatoms <i>Thalassiosira rotula</i> phytosterols 24-methylene cholesterol cytotoxicity cancer |
| url | https://www.mdpi.com/1660-3397/20/10/595 |
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