Immediate effects of maternal deprivation on the (re)activity of the HPA axis differ in CD1 and C57Bl/6J mouse pups
The postnatal development of the mouse is characterised by a period of hypo-responsiveness of the hypothalamic-pituitary-adrenal (HPA) axis to mild stressors. Maternal deprivation (MD) during this period can disrupt the quiescence of the HPA-axis. The present study examined the influence of strain (...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2014-11-01
|
| Series: | Frontiers in Endocrinology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fendo.2014.00190/full |
| _version_ | 1818067503048294400 |
|---|---|
| author | Nikolaos P Daskalakis Nikolaos P Daskalakis Nikolaos P Daskalakis Leo eEnthoven Edwige eSchoonheere Edo Ronald De Kloet Edo Ronald De Kloet Melly S Oitzl Melly S Oitzl |
| author_facet | Nikolaos P Daskalakis Nikolaos P Daskalakis Nikolaos P Daskalakis Leo eEnthoven Edwige eSchoonheere Edo Ronald De Kloet Edo Ronald De Kloet Melly S Oitzl Melly S Oitzl |
| author_sort | Nikolaos P Daskalakis |
| collection | DOAJ |
| description | The postnatal development of the mouse is characterised by a period of hypo-responsiveness of the hypothalamic-pituitary-adrenal (HPA) axis to mild stressors. Maternal deprivation (MD) during this period can disrupt the quiescence of the HPA-axis. The present study examined the influence of strain (outbred CD1 versus inbred C57BL/6J mice) on some central and peripheral components of the HPA-axis in neonatal mice (5 day-old) in the presence of their mother or after 24 h MD (on postnatal day 4) under basal or mild stressful conditions. In the presence of the dam, adrenal corticosterone (CORT) secretion was low in both mouse strains. Compared to CD1 mice, C57BL/6J had lower CORT levels associated with higher ACTH levels and ACTH/CORT ratio (i.e., lower adrenal sensitivity to ACTH), and higher glucocorticoid receptor (GR) mRNA expression in the paraventricular nucleus. Although MD disinhibited the HPA-axis in both strains as reflected by increased basal CORT and ACTH, we found a strain-dependent pattern. MD increased CORT more in C57BL/6J, compared to CD1 mice, together with a lower ACTH/CORT ratio (i.e., higher adrenal sensitivity to ACTH), while GR mRNA was no longer different in the two strains. However, this increased adrenal sensitivity in maternally deprived C57BL/6J mice was not reflected in their CORT response to a subsequent novelty stressor, possibly due to a MD-induced ceiling effect in their steroidogenic capacity.In conclusion, the immediate outcome of MD depends on the genetic background of the mother-infant dyad, suggesting that maybe also the outcome in later-life cannot be generalized. genetic background of the mother-infant dyad, predicting that the outcome in later-life cannot be generalized. |
| first_indexed | 2024-12-10T15:24:43Z |
| format | Article |
| id | doaj.art-54751b7eef0148faa8051e9568c788ea |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-10T15:24:43Z |
| publishDate | 2014-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-54751b7eef0148faa8051e9568c788ea2022-12-22T01:43:35ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922014-11-01510.3389/fendo.2014.00190103110Immediate effects of maternal deprivation on the (re)activity of the HPA axis differ in CD1 and C57Bl/6J mouse pupsNikolaos P Daskalakis0Nikolaos P Daskalakis1Nikolaos P Daskalakis2Leo eEnthoven3Edwige eSchoonheere4Edo Ronald De Kloet5Edo Ronald De Kloet6Melly S Oitzl7Melly S Oitzl8Icahn School of Medicine at Mount SinaiLeiden Academic Center for Drug Research,Leiden University Medical Center,Leiden Academic Center for Drug Research,Leiden Academic Center for Drug Research,Leiden Academic Center for Drug Research,Leiden University Medical Center,Leiden Academic Center for Drug Research,Swammerdam Institute for Life SciencesThe postnatal development of the mouse is characterised by a period of hypo-responsiveness of the hypothalamic-pituitary-adrenal (HPA) axis to mild stressors. Maternal deprivation (MD) during this period can disrupt the quiescence of the HPA-axis. The present study examined the influence of strain (outbred CD1 versus inbred C57BL/6J mice) on some central and peripheral components of the HPA-axis in neonatal mice (5 day-old) in the presence of their mother or after 24 h MD (on postnatal day 4) under basal or mild stressful conditions. In the presence of the dam, adrenal corticosterone (CORT) secretion was low in both mouse strains. Compared to CD1 mice, C57BL/6J had lower CORT levels associated with higher ACTH levels and ACTH/CORT ratio (i.e., lower adrenal sensitivity to ACTH), and higher glucocorticoid receptor (GR) mRNA expression in the paraventricular nucleus. Although MD disinhibited the HPA-axis in both strains as reflected by increased basal CORT and ACTH, we found a strain-dependent pattern. MD increased CORT more in C57BL/6J, compared to CD1 mice, together with a lower ACTH/CORT ratio (i.e., higher adrenal sensitivity to ACTH), while GR mRNA was no longer different in the two strains. However, this increased adrenal sensitivity in maternally deprived C57BL/6J mice was not reflected in their CORT response to a subsequent novelty stressor, possibly due to a MD-induced ceiling effect in their steroidogenic capacity.In conclusion, the immediate outcome of MD depends on the genetic background of the mother-infant dyad, suggesting that maybe also the outcome in later-life cannot be generalized. genetic background of the mother-infant dyad, predicting that the outcome in later-life cannot be generalized.http://journal.frontiersin.org/Journal/10.3389/fendo.2014.00190/fullCorticosteroneMaternal DeprivationACTHgeneticCRHHypothalamic-Pituitary-Adrenal Axis |
| spellingShingle | Nikolaos P Daskalakis Nikolaos P Daskalakis Nikolaos P Daskalakis Leo eEnthoven Edwige eSchoonheere Edo Ronald De Kloet Edo Ronald De Kloet Melly S Oitzl Melly S Oitzl Immediate effects of maternal deprivation on the (re)activity of the HPA axis differ in CD1 and C57Bl/6J mouse pups Frontiers in Endocrinology Corticosterone Maternal Deprivation ACTH genetic CRH Hypothalamic-Pituitary-Adrenal Axis |
| title | Immediate effects of maternal deprivation on the (re)activity of the HPA axis differ in CD1 and C57Bl/6J mouse pups |
| title_full | Immediate effects of maternal deprivation on the (re)activity of the HPA axis differ in CD1 and C57Bl/6J mouse pups |
| title_fullStr | Immediate effects of maternal deprivation on the (re)activity of the HPA axis differ in CD1 and C57Bl/6J mouse pups |
| title_full_unstemmed | Immediate effects of maternal deprivation on the (re)activity of the HPA axis differ in CD1 and C57Bl/6J mouse pups |
| title_short | Immediate effects of maternal deprivation on the (re)activity of the HPA axis differ in CD1 and C57Bl/6J mouse pups |
| title_sort | immediate effects of maternal deprivation on the re activity of the hpa axis differ in cd1 and c57bl 6j mouse pups |
| topic | Corticosterone Maternal Deprivation ACTH genetic CRH Hypothalamic-Pituitary-Adrenal Axis |
| url | http://journal.frontiersin.org/Journal/10.3389/fendo.2014.00190/full |
| work_keys_str_mv | AT nikolaospdaskalakis immediateeffectsofmaternaldeprivationonthereactivityofthehpaaxisdifferincd1andc57bl6jmousepups AT nikolaospdaskalakis immediateeffectsofmaternaldeprivationonthereactivityofthehpaaxisdifferincd1andc57bl6jmousepups AT nikolaospdaskalakis immediateeffectsofmaternaldeprivationonthereactivityofthehpaaxisdifferincd1andc57bl6jmousepups AT leoeenthoven immediateeffectsofmaternaldeprivationonthereactivityofthehpaaxisdifferincd1andc57bl6jmousepups AT edwigeeschoonheere immediateeffectsofmaternaldeprivationonthereactivityofthehpaaxisdifferincd1andc57bl6jmousepups AT edoronalddekloet immediateeffectsofmaternaldeprivationonthereactivityofthehpaaxisdifferincd1andc57bl6jmousepups AT edoronalddekloet immediateeffectsofmaternaldeprivationonthereactivityofthehpaaxisdifferincd1andc57bl6jmousepups AT mellysoitzl immediateeffectsofmaternaldeprivationonthereactivityofthehpaaxisdifferincd1andc57bl6jmousepups AT mellysoitzl immediateeffectsofmaternaldeprivationonthereactivityofthehpaaxisdifferincd1andc57bl6jmousepups |