Novel CO2-encapsulated Pluronic F127 hydrogel for the treatment of Achilles tendon injury
Abstract Nonsurgical treatment and surgical repairment of injured Achilles tendons seldom restore the wounded tendon to its original elasticity and stiffness. Therefore, we hypothesized that the surgically repaired Achilles tendon can achieve satisfactory regeneration by applying multi-drug encapsul...
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| Format: | Article |
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Nature Portfolio
2023-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-49339-z |
| _version_ | 1797388337402609664 |
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| author | Yi-Hsun Yu Chen-Hung Lee Yung-Heng Hsu Ying-Chao Chou Bo-Kui Hong Chao-Tsai Huang Shih-Jung Liu |
| author_facet | Yi-Hsun Yu Chen-Hung Lee Yung-Heng Hsu Ying-Chao Chou Bo-Kui Hong Chao-Tsai Huang Shih-Jung Liu |
| author_sort | Yi-Hsun Yu |
| collection | DOAJ |
| description | Abstract Nonsurgical treatment and surgical repairment of injured Achilles tendons seldom restore the wounded tendon to its original elasticity and stiffness. Therefore, we hypothesized that the surgically repaired Achilles tendon can achieve satisfactory regeneration by applying multi-drug encapsulated hydrogels. In this study, a novel bupivacaine-eluting carbon dioxide-encapsulated Pluronic F127 hydrogel (BC-hydrogel) was developed for the treatment of Achilles tendon injuries. The rheological properties of BC-hydrogel were measured. A high-performance liquid chromatography assay was used to assess the release characteristics of bupivacaine in both in vitro and in vivo settings. Furthermore, the effectiveness of BC-hydrogel in treating torn tendons was examined in a rat model, and histological analyses were conducted. Evidently, the degradable hydrogels continuously eluted bupivacaine for more than 14 days. The animal study results revealed that the BC-hydrogel improved the post-surgery mobility of the animals compared with pristine hydrogels. Histological assay results demonstrated a significant reaction to high vascular endothelial growth factor in the surrounding tissues and expression of collagen I within the repaired tendon. This demonstrates the potential of this novel BC-hydrogel as an effective treatment method for Achilles tendon injuries. |
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| format | Article |
| id | doaj.art-5477a754644a4d22b90a383b4de7585b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T22:39:18Z |
| publishDate | 2023-12-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj.art-5477a754644a4d22b90a383b4de7585b2023-12-17T12:15:25ZengNature PortfolioScientific Reports2045-23222023-12-0113111310.1038/s41598-023-49339-zNovel CO2-encapsulated Pluronic F127 hydrogel for the treatment of Achilles tendon injuryYi-Hsun Yu0Chen-Hung Lee1Yung-Heng Hsu2Ying-Chao Chou3Bo-Kui Hong4Chao-Tsai Huang5Shih-Jung Liu6Department of Orthopedic Surgery, Bone and Joint Research Center, Chang Gung Memorial Hospital at LinkouDivision of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital-Linkou, Chang Gung University College of MedicineDepartment of Orthopedic Surgery, Bone and Joint Research Center, Chang Gung Memorial Hospital at LinkouDepartment of Orthopedic Surgery, Bone and Joint Research Center, Chang Gung Memorial Hospital at LinkouDepartment of Mechanical Engineering, Chang Gung UniversityDepartment of Chemical and Materials Engineering, Tamkang UniversityDepartment of Orthopedic Surgery, Bone and Joint Research Center, Chang Gung Memorial Hospital at LinkouAbstract Nonsurgical treatment and surgical repairment of injured Achilles tendons seldom restore the wounded tendon to its original elasticity and stiffness. Therefore, we hypothesized that the surgically repaired Achilles tendon can achieve satisfactory regeneration by applying multi-drug encapsulated hydrogels. In this study, a novel bupivacaine-eluting carbon dioxide-encapsulated Pluronic F127 hydrogel (BC-hydrogel) was developed for the treatment of Achilles tendon injuries. The rheological properties of BC-hydrogel were measured. A high-performance liquid chromatography assay was used to assess the release characteristics of bupivacaine in both in vitro and in vivo settings. Furthermore, the effectiveness of BC-hydrogel in treating torn tendons was examined in a rat model, and histological analyses were conducted. Evidently, the degradable hydrogels continuously eluted bupivacaine for more than 14 days. The animal study results revealed that the BC-hydrogel improved the post-surgery mobility of the animals compared with pristine hydrogels. Histological assay results demonstrated a significant reaction to high vascular endothelial growth factor in the surrounding tissues and expression of collagen I within the repaired tendon. This demonstrates the potential of this novel BC-hydrogel as an effective treatment method for Achilles tendon injuries.https://doi.org/10.1038/s41598-023-49339-z |
| spellingShingle | Yi-Hsun Yu Chen-Hung Lee Yung-Heng Hsu Ying-Chao Chou Bo-Kui Hong Chao-Tsai Huang Shih-Jung Liu Novel CO2-encapsulated Pluronic F127 hydrogel for the treatment of Achilles tendon injury Scientific Reports |
| title | Novel CO2-encapsulated Pluronic F127 hydrogel for the treatment of Achilles tendon injury |
| title_full | Novel CO2-encapsulated Pluronic F127 hydrogel for the treatment of Achilles tendon injury |
| title_fullStr | Novel CO2-encapsulated Pluronic F127 hydrogel for the treatment of Achilles tendon injury |
| title_full_unstemmed | Novel CO2-encapsulated Pluronic F127 hydrogel for the treatment of Achilles tendon injury |
| title_short | Novel CO2-encapsulated Pluronic F127 hydrogel for the treatment of Achilles tendon injury |
| title_sort | novel co2 encapsulated pluronic f127 hydrogel for the treatment of achilles tendon injury |
| url | https://doi.org/10.1038/s41598-023-49339-z |
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