Anticancer potential of biogenic silver nanoparticles using the stem extract of Commiphora gileadensis against human colon cancer cells
Plant-mediated silver nanoparticles are unique and are considered one of the best nanomaterials used in cancer research. We report a low-cost, eco-friendly process of green synthesis of AgNPs from Commiphora gileadensis stem extracts and evaluated their anticancer potential against colon cancer cell...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2022-04-01
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| Series: | Green Processing and Synthesis |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/gps-2022-0042 |
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| author | Al-Zahrani Sabah Ahmed Bhat Ramesa Shafi Al-Onazi Mona Awad Alwhibi Mona S. Soliman Dina. A. Aljebrin Nora Abdullah Al-Suhaibani Leenah Saleh Al Daihan Sooad |
| author_facet | Al-Zahrani Sabah Ahmed Bhat Ramesa Shafi Al-Onazi Mona Awad Alwhibi Mona S. Soliman Dina. A. Aljebrin Nora Abdullah Al-Suhaibani Leenah Saleh Al Daihan Sooad |
| author_sort | Al-Zahrani Sabah Ahmed |
| collection | DOAJ |
| description | Plant-mediated silver nanoparticles are unique and are considered one of the best nanomaterials used in cancer research. We report a low-cost, eco-friendly process of green synthesis of AgNPs from Commiphora gileadensis stem extracts and evaluated their anticancer potential against colon cancer cell lines HCT-116, HT 29, and SW620. Anticancer activities were performed by an MTT assay and gene expression levels of four genes CHEK1, CHEK2, ATR, and ATM by the real-time polymerase chain reaction. Particles were initially confirmed by UV-visible spectroscopy. The morphology and stability of the particles were examined through TEM, zeta potential, and zeta sizer. GC-MS and FTIR were performed to examine the functional groups. The absorption peak was recorded at 430 nm; the average size recorded by TEM images was 13 nm, while the zeta potential and zeta sizer study showed aggregation in nanoparticles. Compared to C. gileadensis extracts, some of the FT-IR spectrum peaks were sight shifted with some new peaks in C. gileadensis AgNPs. C. gileadensis AgNPs were more toxic against HT29 followed by HTC116 and SW620. Expression levels of most of the genes in HCT116 and HT29 were increased by treatment whereas the gene expression level was least affected in SW620. C. gileadensis AgNPs have anticancer potential and need to be explored in cancer research. |
| first_indexed | 2024-04-12T12:11:11Z |
| format | Article |
| id | doaj.art-5478209196a8482daeff91eddecd136b |
| institution | Directory Open Access Journal |
| issn | 2191-9550 |
| language | English |
| last_indexed | 2024-04-12T12:11:11Z |
| publishDate | 2022-04-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Green Processing and Synthesis |
| spelling | doaj.art-5478209196a8482daeff91eddecd136b2022-12-22T03:33:34ZengDe GruyterGreen Processing and Synthesis2191-95502022-04-0111143544410.1515/gps-2022-0042Anticancer potential of biogenic silver nanoparticles using the stem extract of Commiphora gileadensis against human colon cancer cellsAl-Zahrani Sabah Ahmed0Bhat Ramesa Shafi1Al-Onazi Mona Awad2Alwhibi Mona S.3Soliman Dina. A.4Aljebrin Nora Abdullah5Al-Suhaibani Leenah Saleh6Al Daihan Sooad7Biochemistry Department, College of Science, King Saud University, Riyadh, Saudi ArabiaBiochemistry Department, College of Science, King Saud University, Riyadh, Saudi ArabiaBiochemistry Department, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Botany and Microbiology, King Saud University, Riyadh, Saudi ArabiaDepartment of Botany and Microbiology, King Saud University, Riyadh, Saudi ArabiaBiochemistry Department, College of Science, King Saud University, Riyadh, Saudi ArabiaBiochemistry Department, College of Science, King Saud University, Riyadh, Saudi ArabiaBiochemistry Department, College of Science, King Saud University, Riyadh, Saudi ArabiaPlant-mediated silver nanoparticles are unique and are considered one of the best nanomaterials used in cancer research. We report a low-cost, eco-friendly process of green synthesis of AgNPs from Commiphora gileadensis stem extracts and evaluated their anticancer potential against colon cancer cell lines HCT-116, HT 29, and SW620. Anticancer activities were performed by an MTT assay and gene expression levels of four genes CHEK1, CHEK2, ATR, and ATM by the real-time polymerase chain reaction. Particles were initially confirmed by UV-visible spectroscopy. The morphology and stability of the particles were examined through TEM, zeta potential, and zeta sizer. GC-MS and FTIR were performed to examine the functional groups. The absorption peak was recorded at 430 nm; the average size recorded by TEM images was 13 nm, while the zeta potential and zeta sizer study showed aggregation in nanoparticles. Compared to C. gileadensis extracts, some of the FT-IR spectrum peaks were sight shifted with some new peaks in C. gileadensis AgNPs. C. gileadensis AgNPs were more toxic against HT29 followed by HTC116 and SW620. Expression levels of most of the genes in HCT116 and HT29 were increased by treatment whereas the gene expression level was least affected in SW620. C. gileadensis AgNPs have anticancer potential and need to be explored in cancer research.https://doi.org/10.1515/gps-2022-0042commiphora gileadensissilver nanoparticlescytotoxicityhuman colon cancer cell lines |
| spellingShingle | Al-Zahrani Sabah Ahmed Bhat Ramesa Shafi Al-Onazi Mona Awad Alwhibi Mona S. Soliman Dina. A. Aljebrin Nora Abdullah Al-Suhaibani Leenah Saleh Al Daihan Sooad Anticancer potential of biogenic silver nanoparticles using the stem extract of Commiphora gileadensis against human colon cancer cells Green Processing and Synthesis commiphora gileadensis silver nanoparticles cytotoxicity human colon cancer cell lines |
| title | Anticancer potential of biogenic silver nanoparticles using the stem extract of Commiphora gileadensis against human colon cancer cells |
| title_full | Anticancer potential of biogenic silver nanoparticles using the stem extract of Commiphora gileadensis against human colon cancer cells |
| title_fullStr | Anticancer potential of biogenic silver nanoparticles using the stem extract of Commiphora gileadensis against human colon cancer cells |
| title_full_unstemmed | Anticancer potential of biogenic silver nanoparticles using the stem extract of Commiphora gileadensis against human colon cancer cells |
| title_short | Anticancer potential of biogenic silver nanoparticles using the stem extract of Commiphora gileadensis against human colon cancer cells |
| title_sort | anticancer potential of biogenic silver nanoparticles using the stem extract of commiphora gileadensis against human colon cancer cells |
| topic | commiphora gileadensis silver nanoparticles cytotoxicity human colon cancer cell lines |
| url | https://doi.org/10.1515/gps-2022-0042 |
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