Impact of Acetate in Reduction of Perchlorate by Mixed Microbial Culture under the Influence of Nitrate and Sulfate
The biological reduction of slow degradation contaminants such as perchlorate (ClO<sub>4</sub><sup>−</sup>) is considered to be a promising water treatment technology. The process is based on the ability of a specific mixed microbial culture to use perchlorate as an electron...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/18/10608 |
| _version_ | 1797487231863095296 |
|---|---|
| author | Hosung Yu Kang Hoon Lee Jae-Woo Park |
| author_facet | Hosung Yu Kang Hoon Lee Jae-Woo Park |
| author_sort | Hosung Yu |
| collection | DOAJ |
| description | The biological reduction of slow degradation contaminants such as perchlorate (ClO<sub>4</sub><sup>−</sup>) is considered to be a promising water treatment technology. The process is based on the ability of a specific mixed microbial culture to use perchlorate as an electron acceptor in the absence of oxygen. In this study, batch experiments were conducted to investigate the effect of nitrate on perchlorate reduction, the kinetic parameters of the Monod equation and the optimal ratio of acetate to perchlorate for the perchlorate reducing bacterial consortium. The results of this study suggest that acclimated microbial cultures can be applied to treat wastewater containing high concentrations of perchlorate. Reactor experiments were carried out with different hydraulic retention times (HRTs) to determine the optimal operating conditions. A fixed optimal HRT and the effect of nitrate on perchlorate reduction were investigated with various concentrations of the electron donor. The results showed that perchlorate reduction occurred after nitrate removal. Moreover, the presence of sulfate in wastewater had no effect on the perchlorate reduction. However, it had little effect on biomass concentration in the presence of nitrate during exposure to a mixed microbial culture, considering the nitrate as the inhibitor of perchlorate reduction by reducing the degradation rate. The batch scale experiment results illustrated that for efficient operation of perchlorate reduction, the optimal acetate to perchlorate ratio of 1.4:1.0 would be enough. Moreover, these experiments found the following results: the kinetic parameters equivalent to Y = 0.281 mg biomass/mg perchlorate, <i>K<sub>s</sub></i> = 37.619 mg/L and q<sub>max</sub> = 0.042 mg perchlorate/mg biomass/h. In addition, anoxic–aerobic experimental reactor results verify the optimal HRT of 6 h for continuous application. Furthermore, it also illustrated that using 600 mg/L of acetate as a carbon source is responsible for 100% of nitrate reduction with less than 50% of the perchlorate reduction, whereas at 1000 mg/L acetate, approximately 100% reduction was recorded. |
| first_indexed | 2024-03-09T23:45:42Z |
| format | Article |
| id | doaj.art-5479f7101f5340b08e00afc2a5b50f5a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:45:42Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5479f7101f5340b08e00afc2a5b50f5a2023-11-23T16:44:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123181060810.3390/ijms231810608Impact of Acetate in Reduction of Perchlorate by Mixed Microbial Culture under the Influence of Nitrate and SulfateHosung Yu0Kang Hoon Lee1Jae-Woo Park2Department of Civil and Environmental Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Energy and Environmental Engineering, The Catholic University of Korea, 43 Jibong-ro, Bucheon-si 14662, KoreaDepartment of Civil and Environmental Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaThe biological reduction of slow degradation contaminants such as perchlorate (ClO<sub>4</sub><sup>−</sup>) is considered to be a promising water treatment technology. The process is based on the ability of a specific mixed microbial culture to use perchlorate as an electron acceptor in the absence of oxygen. In this study, batch experiments were conducted to investigate the effect of nitrate on perchlorate reduction, the kinetic parameters of the Monod equation and the optimal ratio of acetate to perchlorate for the perchlorate reducing bacterial consortium. The results of this study suggest that acclimated microbial cultures can be applied to treat wastewater containing high concentrations of perchlorate. Reactor experiments were carried out with different hydraulic retention times (HRTs) to determine the optimal operating conditions. A fixed optimal HRT and the effect of nitrate on perchlorate reduction were investigated with various concentrations of the electron donor. The results showed that perchlorate reduction occurred after nitrate removal. Moreover, the presence of sulfate in wastewater had no effect on the perchlorate reduction. However, it had little effect on biomass concentration in the presence of nitrate during exposure to a mixed microbial culture, considering the nitrate as the inhibitor of perchlorate reduction by reducing the degradation rate. The batch scale experiment results illustrated that for efficient operation of perchlorate reduction, the optimal acetate to perchlorate ratio of 1.4:1.0 would be enough. Moreover, these experiments found the following results: the kinetic parameters equivalent to Y = 0.281 mg biomass/mg perchlorate, <i>K<sub>s</sub></i> = 37.619 mg/L and q<sub>max</sub> = 0.042 mg perchlorate/mg biomass/h. In addition, anoxic–aerobic experimental reactor results verify the optimal HRT of 6 h for continuous application. Furthermore, it also illustrated that using 600 mg/L of acetate as a carbon source is responsible for 100% of nitrate reduction with less than 50% of the perchlorate reduction, whereas at 1000 mg/L acetate, approximately 100% reduction was recorded.https://www.mdpi.com/1422-0067/23/18/10608perchloratemixed microbial culturebio reductionMonod equationhydraulic retention time (HRT) |
| spellingShingle | Hosung Yu Kang Hoon Lee Jae-Woo Park Impact of Acetate in Reduction of Perchlorate by Mixed Microbial Culture under the Influence of Nitrate and Sulfate International Journal of Molecular Sciences perchlorate mixed microbial culture bio reduction Monod equation hydraulic retention time (HRT) |
| title | Impact of Acetate in Reduction of Perchlorate by Mixed Microbial Culture under the Influence of Nitrate and Sulfate |
| title_full | Impact of Acetate in Reduction of Perchlorate by Mixed Microbial Culture under the Influence of Nitrate and Sulfate |
| title_fullStr | Impact of Acetate in Reduction of Perchlorate by Mixed Microbial Culture under the Influence of Nitrate and Sulfate |
| title_full_unstemmed | Impact of Acetate in Reduction of Perchlorate by Mixed Microbial Culture under the Influence of Nitrate and Sulfate |
| title_short | Impact of Acetate in Reduction of Perchlorate by Mixed Microbial Culture under the Influence of Nitrate and Sulfate |
| title_sort | impact of acetate in reduction of perchlorate by mixed microbial culture under the influence of nitrate and sulfate |
| topic | perchlorate mixed microbial culture bio reduction Monod equation hydraulic retention time (HRT) |
| url | https://www.mdpi.com/1422-0067/23/18/10608 |
| work_keys_str_mv | AT hosungyu impactofacetateinreductionofperchloratebymixedmicrobialcultureundertheinfluenceofnitrateandsulfate AT kanghoonlee impactofacetateinreductionofperchloratebymixedmicrobialcultureundertheinfluenceofnitrateandsulfate AT jaewoopark impactofacetateinreductionofperchloratebymixedmicrobialcultureundertheinfluenceofnitrateandsulfate |