Influence of incubation temperature and embryonic motility on the growth of members of Caiman yacare (Daudin, 1802)
Abstract This study aimed to evaluate whether skeletal development of the Pantanal Caiman (Caiman yacare) is similarly influenced by temperature variation and controlled increases in embryo motility. All eggs were incubated at 90% humidity and 29 °C for the first 45 days. Thereafter, the incubation...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Instituto Internacional de Ecologia
2021-12-01
|
| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000100148&tlng=en |
| _version_ | 1798019278376534016 |
|---|---|
| author | L. G. Gomes M. B. Stocco N. P. de Sousa A. C. Martini T. O. Morgado P. R. Spiller L. F. B. Moreira R. L. de Souza |
| author_facet | L. G. Gomes M. B. Stocco N. P. de Sousa A. C. Martini T. O. Morgado P. R. Spiller L. F. B. Moreira R. L. de Souza |
| author_sort | L. G. Gomes |
| collection | DOAJ |
| description | Abstract This study aimed to evaluate whether skeletal development of the Pantanal Caiman (Caiman yacare) is similarly influenced by temperature variation and controlled increases in embryo motility. All eggs were incubated at 90% humidity and 29 °C for the first 45 days. Thereafter, the incubation temperature was either maintained at 29 °C and embryos were treated with 4-aminopyridine (4-AP) on days 46, 47, 48, and 49 (Group I, 29 °C 4-AP, n = 15); maintained at 29 °C (n = 14; Group II); or at 33 °C (n = 14, Group III). Embryonic movement was measured using an Egg Buddy® digital monitor on days 30, 35, 42, 49, 56, and 60, at which point embryos were euthanized and samples were collected for analysis. No differences were observed between groups with varying incubation temperatures. In contrast, embryonic motility was greater in embryos treated with 4-AP (P < 0.001) on day 49, and this was associated with higher proportions of snout-vent and hand lengths. This study demonstrates for the first time that pharmacologically induced increases in embryo motility result in phenotypic changes to the proportion of elements during prenatal ontogeny, thereby effectively altering the adaptation of the species to specific environments. |
| first_indexed | 2024-04-11T16:38:15Z |
| format | Article |
| id | doaj.art-547a90bb85db45eaae1197d14a36fc1a |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-04-11T16:38:15Z |
| publishDate | 2021-12-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-547a90bb85db45eaae1197d14a36fc1a2022-12-22T04:13:44ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752021-12-018410.1590/1519-6984.252845Influence of incubation temperature and embryonic motility on the growth of members of Caiman yacare (Daudin, 1802)L. G. Gomeshttps://orcid.org/0000-0003-0909-2426M. B. Stoccohttps://orcid.org/0000-0003-2069-1788N. P. de Sousahttps://orcid.org/0000-0002-2798-1588A. C. Martinihttps://orcid.org/0000-0002-2553-3685T. O. Morgadohttps://orcid.org/0000-0002-2974-3241P. R. Spillerhttps://orcid.org/0000-0002-4659-4647L. F. B. Moreirahttps://orcid.org/0000-0002-2753-9933R. L. de Souzahttps://orcid.org/0000-0002-8079-7181Abstract This study aimed to evaluate whether skeletal development of the Pantanal Caiman (Caiman yacare) is similarly influenced by temperature variation and controlled increases in embryo motility. All eggs were incubated at 90% humidity and 29 °C for the first 45 days. Thereafter, the incubation temperature was either maintained at 29 °C and embryos were treated with 4-aminopyridine (4-AP) on days 46, 47, 48, and 49 (Group I, 29 °C 4-AP, n = 15); maintained at 29 °C (n = 14; Group II); or at 33 °C (n = 14, Group III). Embryonic movement was measured using an Egg Buddy® digital monitor on days 30, 35, 42, 49, 56, and 60, at which point embryos were euthanized and samples were collected for analysis. No differences were observed between groups with varying incubation temperatures. In contrast, embryonic motility was greater in embryos treated with 4-AP (P < 0.001) on day 49, and this was associated with higher proportions of snout-vent and hand lengths. This study demonstrates for the first time that pharmacologically induced increases in embryo motility result in phenotypic changes to the proportion of elements during prenatal ontogeny, thereby effectively altering the adaptation of the species to specific environments.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000100148&tlng=enembryonic movementtemperaturePantanal Caiman |
| spellingShingle | L. G. Gomes M. B. Stocco N. P. de Sousa A. C. Martini T. O. Morgado P. R. Spiller L. F. B. Moreira R. L. de Souza Influence of incubation temperature and embryonic motility on the growth of members of Caiman yacare (Daudin, 1802) Brazilian Journal of Biology embryonic movement temperature Pantanal Caiman |
| title | Influence of incubation temperature and embryonic motility on the growth of members of Caiman yacare (Daudin, 1802) |
| title_full | Influence of incubation temperature and embryonic motility on the growth of members of Caiman yacare (Daudin, 1802) |
| title_fullStr | Influence of incubation temperature and embryonic motility on the growth of members of Caiman yacare (Daudin, 1802) |
| title_full_unstemmed | Influence of incubation temperature and embryonic motility on the growth of members of Caiman yacare (Daudin, 1802) |
| title_short | Influence of incubation temperature and embryonic motility on the growth of members of Caiman yacare (Daudin, 1802) |
| title_sort | influence of incubation temperature and embryonic motility on the growth of members of caiman yacare daudin 1802 |
| topic | embryonic movement temperature Pantanal Caiman |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000100148&tlng=en |
| work_keys_str_mv | AT lggomes influenceofincubationtemperatureandembryonicmotilityonthegrowthofmembersofcaimanyacaredaudin1802 AT mbstocco influenceofincubationtemperatureandembryonicmotilityonthegrowthofmembersofcaimanyacaredaudin1802 AT npdesousa influenceofincubationtemperatureandembryonicmotilityonthegrowthofmembersofcaimanyacaredaudin1802 AT acmartini influenceofincubationtemperatureandembryonicmotilityonthegrowthofmembersofcaimanyacaredaudin1802 AT tomorgado influenceofincubationtemperatureandembryonicmotilityonthegrowthofmembersofcaimanyacaredaudin1802 AT prspiller influenceofincubationtemperatureandembryonicmotilityonthegrowthofmembersofcaimanyacaredaudin1802 AT lfbmoreira influenceofincubationtemperatureandembryonicmotilityonthegrowthofmembersofcaimanyacaredaudin1802 AT rldesouza influenceofincubationtemperatureandembryonicmotilityonthegrowthofmembersofcaimanyacaredaudin1802 |