Comparison between anal cytology, high-resolution anoscopy and HPV DNA genotyping by polymerase chain reaction in the post-treatment follow-up of condylomata acuminata
ABSTRACT Aim: to evaluate the presence of subclinical HPV-induced anal lesions with anal cytology, High-Resolution Anoscopy (HRA) and HPV genotyping by polymerase chain reaction (PCR) in the follow-up of treated condylomata acuminata (CA). Methods: seventy-nine male patients were included. One mo...
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Colégio Brasileiro de Cirurgiões
2020-07-01
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| Series: | Revista do Colégio Brasileiro de Cirurgiões |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912020000100180&tlng=en |
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| author | LUIS ROBERTO NADAL SARHAN SYDNEY SAAD GASPAR JESUS LOPES FILHO HELENA PASSARELLI GIROUD JOAQUIM THIAGO DA SILVEIRA MANZIONE CARMEN RUTH MANZIONE SIDNEY ROBERTO NADAL |
| author_facet | LUIS ROBERTO NADAL SARHAN SYDNEY SAAD GASPAR JESUS LOPES FILHO HELENA PASSARELLI GIROUD JOAQUIM THIAGO DA SILVEIRA MANZIONE CARMEN RUTH MANZIONE SIDNEY ROBERTO NADAL |
| author_sort | LUIS ROBERTO NADAL |
| collection | DOAJ |
| description | ABSTRACT Aim: to evaluate the presence of subclinical HPV-induced anal lesions with anal cytology, High-Resolution Anoscopy (HRA) and HPV genotyping by polymerase chain reaction (PCR) in the follow-up of treated condylomata acuminata (CA). Methods: seventy-nine male patients were included. One month after anal CA eradication, the patients underwent brush samples collection for anal cytology and PCR, and HRA with biopsy of acetowhite lesions. These methods were compared within all patients and between groups, according to Human Immunodeficiency Virus (HIV) infection status: HIV-negative; HIV-positive with TCD4 count above and below 350 cells/mm3. Results: the most frequent HPV types were 6 and 16. HPV DNA was isolated in 92%. HIV infection was associated with a higher number of oncogenic HPV types (p=0.038). All patients with negative PCR had negative HRA and cytology. There were no differences in cytological, HRA or histopathological findings between groups. Conclusion: the association of the findings of cytopathology, HRA and genotyping of HPV refined the diagnosis of HPV-induced lesions. The degree of immunodeficiency was not associated with increase in remnant HPV-induced anal lesions. |
| first_indexed | 2024-12-13T22:28:21Z |
| format | Article |
| id | doaj.art-547ba219acc4416e941d2024c3537f14 |
| institution | Directory Open Access Journal |
| issn | 1809-4546 |
| language | English |
| last_indexed | 2024-12-13T22:28:21Z |
| publishDate | 2020-07-01 |
| publisher | Colégio Brasileiro de Cirurgiões |
| record_format | Article |
| series | Revista do Colégio Brasileiro de Cirurgiões |
| spelling | doaj.art-547ba219acc4416e941d2024c3537f142022-12-21T23:29:09ZengColégio Brasileiro de CirurgiõesRevista do Colégio Brasileiro de Cirurgiões1809-45462020-07-014710.1590/0100-6991e-20202543Comparison between anal cytology, high-resolution anoscopy and HPV DNA genotyping by polymerase chain reaction in the post-treatment follow-up of condylomata acuminataLUIS ROBERTO NADALhttps://orcid.org/0000-0003-2980-9869SARHAN SYDNEY SAADGASPAR JESUS LOPES FILHOHELENA PASSARELLI GIROUD JOAQUIMTHIAGO DA SILVEIRA MANZIONECARMEN RUTH MANZIONESIDNEY ROBERTO NADALABSTRACT Aim: to evaluate the presence of subclinical HPV-induced anal lesions with anal cytology, High-Resolution Anoscopy (HRA) and HPV genotyping by polymerase chain reaction (PCR) in the follow-up of treated condylomata acuminata (CA). Methods: seventy-nine male patients were included. One month after anal CA eradication, the patients underwent brush samples collection for anal cytology and PCR, and HRA with biopsy of acetowhite lesions. These methods were compared within all patients and between groups, according to Human Immunodeficiency Virus (HIV) infection status: HIV-negative; HIV-positive with TCD4 count above and below 350 cells/mm3. Results: the most frequent HPV types were 6 and 16. HPV DNA was isolated in 92%. HIV infection was associated with a higher number of oncogenic HPV types (p=0.038). All patients with negative PCR had negative HRA and cytology. There were no differences in cytological, HRA or histopathological findings between groups. Conclusion: the association of the findings of cytopathology, HRA and genotyping of HPV refined the diagnosis of HPV-induced lesions. The degree of immunodeficiency was not associated with increase in remnant HPV-induced anal lesions.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912020000100180&tlng=enPapillomavirus InfectionAnal CanalCondylomata AcuminataPolymerase Chain ReactionHigh-Resolution AnoscopyAnal Intraepithelial Neoplasia |
| spellingShingle | LUIS ROBERTO NADAL SARHAN SYDNEY SAAD GASPAR JESUS LOPES FILHO HELENA PASSARELLI GIROUD JOAQUIM THIAGO DA SILVEIRA MANZIONE CARMEN RUTH MANZIONE SIDNEY ROBERTO NADAL Comparison between anal cytology, high-resolution anoscopy and HPV DNA genotyping by polymerase chain reaction in the post-treatment follow-up of condylomata acuminata Revista do Colégio Brasileiro de Cirurgiões Papillomavirus Infection Anal Canal Condylomata Acuminata Polymerase Chain Reaction High-Resolution Anoscopy Anal Intraepithelial Neoplasia |
| title | Comparison between anal cytology, high-resolution anoscopy and HPV DNA genotyping by polymerase chain reaction in the post-treatment follow-up of condylomata acuminata |
| title_full | Comparison between anal cytology, high-resolution anoscopy and HPV DNA genotyping by polymerase chain reaction in the post-treatment follow-up of condylomata acuminata |
| title_fullStr | Comparison between anal cytology, high-resolution anoscopy and HPV DNA genotyping by polymerase chain reaction in the post-treatment follow-up of condylomata acuminata |
| title_full_unstemmed | Comparison between anal cytology, high-resolution anoscopy and HPV DNA genotyping by polymerase chain reaction in the post-treatment follow-up of condylomata acuminata |
| title_short | Comparison between anal cytology, high-resolution anoscopy and HPV DNA genotyping by polymerase chain reaction in the post-treatment follow-up of condylomata acuminata |
| title_sort | comparison between anal cytology high resolution anoscopy and hpv dna genotyping by polymerase chain reaction in the post treatment follow up of condylomata acuminata |
| topic | Papillomavirus Infection Anal Canal Condylomata Acuminata Polymerase Chain Reaction High-Resolution Anoscopy Anal Intraepithelial Neoplasia |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912020000100180&tlng=en |
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