miR-542-3p Contributes to the HK2-Mediated High Glycolytic Phenotype in Human Glioma Cells
(1) Background: The elevation of glucose metabolism is linked to high-grade gliomas such as glioblastoma multiforme (GBM). The high glycolytic phenotype is associated with cellular proliferation and resistance to treatment with chemotherapeutic agents in GBM. MicroRNA-542-3p (miR-542-3p) has been im...
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MDPI AG
2021-04-01
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| author | Junhyung Kim Min Woo Park Young Joon Park Ju Won Ahn Jeong Min Sim Suwan Kim Jinhyung Heo Ji Hun Jeong Mihye Lee Jaejoon Lim Jong-Seok Moon |
| author_facet | Junhyung Kim Min Woo Park Young Joon Park Ju Won Ahn Jeong Min Sim Suwan Kim Jinhyung Heo Ji Hun Jeong Mihye Lee Jaejoon Lim Jong-Seok Moon |
| author_sort | Junhyung Kim |
| collection | DOAJ |
| description | (1) Background: The elevation of glucose metabolism is linked to high-grade gliomas such as glioblastoma multiforme (GBM). The high glycolytic phenotype is associated with cellular proliferation and resistance to treatment with chemotherapeutic agents in GBM. MicroRNA-542-3p (miR-542-3p) has been implicated in several tumors including gliomas. However, the role of miR-542-3p in glucose metabolism in human gliomas remains unclear; (2) Methods: We measured the levels of cellular proliferation in human glioma cells. We measured the glycolytic activity in miR-542-3p knockdown and over-expressed human glioma cells. We measured the levels of miR-542-3p and HK2 in glioma tissues from patients with low- and high-grade gliomas using imaging analysis; (3) Results: We show that knockdown of miR-542-3p significantly suppressed cellular proliferation in human glioma cells. Knockdown of miR-542-3p suppressed HK2-induced glycolytic activity in human glioma cells. Consistently, over-expression of miR-542-3p increased HK2-induced glycolytic activity in human glioma cells. The levels of miR-542-3p and HK2 were significantly elevated in glioma tissues of patients with high-grade gliomas relative to that in low-grade gliomas. The elevation of HK2 levels in patients with high-grade gliomas were positively correlated with the high levels of miR-542-3p in GBM and low-grade gliomas (LGG) based on the datasets from the Cancer Genome Atlas (TCGA) database. Moreover, the high levels of miR-542-3p were associated with poor survival rate in the TCGA database; (4) Conclusions: miR-542-3p contributes to the HK2-mediated high glycolytic phenotype in human glioma cells. |
| first_indexed | 2024-03-10T12:01:47Z |
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| id | doaj.art-547cb7dd5c5d44998471b0e01d6a45e5 |
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| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T12:01:47Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-547cb7dd5c5d44998471b0e01d6a45e52023-11-21T16:52:02ZengMDPI AGGenes2073-44252021-04-0112563310.3390/genes12050633miR-542-3p Contributes to the HK2-Mediated High Glycolytic Phenotype in Human Glioma CellsJunhyung Kim0Min Woo Park1Young Joon Park2Ju Won Ahn3Jeong Min Sim4Suwan Kim5Jinhyung Heo6Ji Hun Jeong7Mihye Lee8Jaejoon Lim9Jong-Seok Moon10Department of Integrated Biomedical Science, Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Chungcheongnam-do, Cheonan 31151, KoreaDepartment of Integrated Biomedical Science, Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Chungcheongnam-do, Cheonan 31151, KoreaBundang CHA Medical Center, Department of Neurosurgery, CHA University, Yatap-dong 59, Seongnam 13496, KoreaBundang CHA Medical Center, Department of Neurosurgery, CHA University, Yatap-dong 59, Seongnam 13496, KoreaBundang CHA Medical Center, Department of Neurosurgery, CHA University, Yatap-dong 59, Seongnam 13496, KoreaBundang CHA Medical Center, Department of Neurosurgery, CHA University, Yatap-dong 59, Seongnam 13496, KoreaBundang CHA Medical Center, Department of Pathology, CHA University, Yatap-dong 59, Seongnam 13496, KoreaDepartment of Integrated Biomedical Science, Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Chungcheongnam-do, Cheonan 31151, KoreaDepartment of Integrated Biomedical Science, Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Chungcheongnam-do, Cheonan 31151, KoreaBundang CHA Medical Center, Department of Neurosurgery, CHA University, Yatap-dong 59, Seongnam 13496, KoreaDepartment of Integrated Biomedical Science, Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Chungcheongnam-do, Cheonan 31151, Korea(1) Background: The elevation of glucose metabolism is linked to high-grade gliomas such as glioblastoma multiforme (GBM). The high glycolytic phenotype is associated with cellular proliferation and resistance to treatment with chemotherapeutic agents in GBM. MicroRNA-542-3p (miR-542-3p) has been implicated in several tumors including gliomas. However, the role of miR-542-3p in glucose metabolism in human gliomas remains unclear; (2) Methods: We measured the levels of cellular proliferation in human glioma cells. We measured the glycolytic activity in miR-542-3p knockdown and over-expressed human glioma cells. We measured the levels of miR-542-3p and HK2 in glioma tissues from patients with low- and high-grade gliomas using imaging analysis; (3) Results: We show that knockdown of miR-542-3p significantly suppressed cellular proliferation in human glioma cells. Knockdown of miR-542-3p suppressed HK2-induced glycolytic activity in human glioma cells. Consistently, over-expression of miR-542-3p increased HK2-induced glycolytic activity in human glioma cells. The levels of miR-542-3p and HK2 were significantly elevated in glioma tissues of patients with high-grade gliomas relative to that in low-grade gliomas. The elevation of HK2 levels in patients with high-grade gliomas were positively correlated with the high levels of miR-542-3p in GBM and low-grade gliomas (LGG) based on the datasets from the Cancer Genome Atlas (TCGA) database. Moreover, the high levels of miR-542-3p were associated with poor survival rate in the TCGA database; (4) Conclusions: miR-542-3p contributes to the HK2-mediated high glycolytic phenotype in human glioma cells.https://www.mdpi.com/2073-4425/12/5/633miR-542-3pcellular proliferationhexokinase 2glycolysisglioma |
| spellingShingle | Junhyung Kim Min Woo Park Young Joon Park Ju Won Ahn Jeong Min Sim Suwan Kim Jinhyung Heo Ji Hun Jeong Mihye Lee Jaejoon Lim Jong-Seok Moon miR-542-3p Contributes to the HK2-Mediated High Glycolytic Phenotype in Human Glioma Cells Genes miR-542-3p cellular proliferation hexokinase 2 glycolysis glioma |
| title | miR-542-3p Contributes to the HK2-Mediated High Glycolytic Phenotype in Human Glioma Cells |
| title_full | miR-542-3p Contributes to the HK2-Mediated High Glycolytic Phenotype in Human Glioma Cells |
| title_fullStr | miR-542-3p Contributes to the HK2-Mediated High Glycolytic Phenotype in Human Glioma Cells |
| title_full_unstemmed | miR-542-3p Contributes to the HK2-Mediated High Glycolytic Phenotype in Human Glioma Cells |
| title_short | miR-542-3p Contributes to the HK2-Mediated High Glycolytic Phenotype in Human Glioma Cells |
| title_sort | mir 542 3p contributes to the hk2 mediated high glycolytic phenotype in human glioma cells |
| topic | miR-542-3p cellular proliferation hexokinase 2 glycolysis glioma |
| url | https://www.mdpi.com/2073-4425/12/5/633 |
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