Quantification of circadian rhythms in single cells.
Bioluminescence techniques allow accurate monitoring of the circadian clock in single cells. We have analyzed bioluminescence data of Per gene expression in mouse SCN neurons and fibroblasts. From these data, we extracted parameters such as damping rate and noise intensity using two simple mathemati...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-11-01
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| Series: | PLoS Computational Biology |
| Online Access: | http://europepmc.org/articles/PMC2776301?pdf=render |
| _version_ | 1818359705890717696 |
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| author | Pål O Westermark David K Welsh Hitoshi Okamura Hanspeter Herzel |
| author_facet | Pål O Westermark David K Welsh Hitoshi Okamura Hanspeter Herzel |
| author_sort | Pål O Westermark |
| collection | DOAJ |
| description | Bioluminescence techniques allow accurate monitoring of the circadian clock in single cells. We have analyzed bioluminescence data of Per gene expression in mouse SCN neurons and fibroblasts. From these data, we extracted parameters such as damping rate and noise intensity using two simple mathematical models, one describing a damped oscillator driven by noise, and one describing a self-sustained noisy oscillator. Both models describe the data well and enabled us to quantitatively characterize both wild-type cells and several mutants. It has been suggested that the circadian clock is self-sustained at the single cell level, but we conclude that present data are not sufficient to determine whether the circadian clock of single SCN neurons and fibroblasts is a damped or a self-sustained oscillator. We show how to settle this question, however, by testing the models' predictions of different phases and amplitudes in response to a periodic entrainment signal (zeitgeber). |
| first_indexed | 2024-12-13T20:49:09Z |
| format | Article |
| id | doaj.art-547d2239cc7a40db9c7cb1989afe8f13 |
| institution | Directory Open Access Journal |
| issn | 1553-734X 1553-7358 |
| language | English |
| last_indexed | 2024-12-13T20:49:09Z |
| publishDate | 2009-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Computational Biology |
| spelling | doaj.art-547d2239cc7a40db9c7cb1989afe8f132022-12-21T23:31:55ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582009-11-01511e100058010.1371/journal.pcbi.1000580Quantification of circadian rhythms in single cells.Pål O WestermarkDavid K WelshHitoshi OkamuraHanspeter HerzelBioluminescence techniques allow accurate monitoring of the circadian clock in single cells. We have analyzed bioluminescence data of Per gene expression in mouse SCN neurons and fibroblasts. From these data, we extracted parameters such as damping rate and noise intensity using two simple mathematical models, one describing a damped oscillator driven by noise, and one describing a self-sustained noisy oscillator. Both models describe the data well and enabled us to quantitatively characterize both wild-type cells and several mutants. It has been suggested that the circadian clock is self-sustained at the single cell level, but we conclude that present data are not sufficient to determine whether the circadian clock of single SCN neurons and fibroblasts is a damped or a self-sustained oscillator. We show how to settle this question, however, by testing the models' predictions of different phases and amplitudes in response to a periodic entrainment signal (zeitgeber).http://europepmc.org/articles/PMC2776301?pdf=render |
| spellingShingle | Pål O Westermark David K Welsh Hitoshi Okamura Hanspeter Herzel Quantification of circadian rhythms in single cells. PLoS Computational Biology |
| title | Quantification of circadian rhythms in single cells. |
| title_full | Quantification of circadian rhythms in single cells. |
| title_fullStr | Quantification of circadian rhythms in single cells. |
| title_full_unstemmed | Quantification of circadian rhythms in single cells. |
| title_short | Quantification of circadian rhythms in single cells. |
| title_sort | quantification of circadian rhythms in single cells |
| url | http://europepmc.org/articles/PMC2776301?pdf=render |
| work_keys_str_mv | AT palowestermark quantificationofcircadianrhythmsinsinglecells AT davidkwelsh quantificationofcircadianrhythmsinsinglecells AT hitoshiokamura quantificationofcircadianrhythmsinsinglecells AT hanspeterherzel quantificationofcircadianrhythmsinsinglecells |