Persimmon Leaves (<i>Diospyros kaki</i>) Extract Enhances the Viability of Human Corneal Endothelial Cells by Improving Na<sup>+</sup>-K<sup>+</sup>-ATPase Activity
The Na<sup>+</sup>/K<sup>+</sup>-ATPase, present in the basolateral membrane of human corneal endothelial cells (HCECs), is known to play an important role for corneal transparency. Na<sup>+</sup>/K<sup>+</sup>-ATPase dysfunction is one of the major ca...
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MDPI AG
2022-01-01
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| Online Access: | https://www.mdpi.com/1424-8247/15/1/72 |
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| author | Ramsha Afzal Hyung Bin Hwang |
| author_facet | Ramsha Afzal Hyung Bin Hwang |
| author_sort | Ramsha Afzal |
| collection | DOAJ |
| description | The Na<sup>+</sup>/K<sup>+</sup>-ATPase, present in the basolateral membrane of human corneal endothelial cells (HCECs), is known to play an important role for corneal transparency. Na<sup>+</sup>/K<sup>+</sup>-ATPase dysfunction is one of the major causes of corneal decompensation. The ethanol extract of <i>Diospyros kaki</i> (EEDK) has been reported to increase corneal cell viability. Thus, we treated HCECs with EEDK and studied its effects on HCECs survival and Na<sup>+</sup>/K<sup>+</sup>-ATPase against cytotoxic drugs like staurosporine (ST) and ouabain (OU). Firstly, survival assays, (MTT assay and live dead-imaging) showed that decreased HCECs viability by ST and OU was significantly recovered by EEDK co-treatment. Secondly, Na<sup>+</sup>/K<sup>+</sup>-ATPase activity assays revealed that EEDK enhanced Na<sup>+</sup>/K<sup>+</sup>-ATPase enzymatic activity (* <i>p</i> < 0.01) with/without ST and OU. Finally, Na<sup>+</sup>/K<sup>+</sup>-ATPase expression analysis (Western Blot and confocal microscopy) demonstrated that EEDK treatment with/without ST and OU facilitates Na<sup>+</sup>/K<sup>+</sup>-ATPase expression in HCECs. Taken together, our findings led us to the conclusion that EEDK might aid HCECs survival in vitro by increasing the activity and expression of Na<sup>+</sup>/K<sup>+</sup>-ATPase enzyme. Since Na<sup>+</sup>/K<sup>+</sup>-ATPase activity is important to maintain cellular function of HCECs, we suggest that EEDK can be a potential effective agent against corneal edema and related corneal disorders. |
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| spelling | doaj.art-547ec19919a745b1a2e26fa1de0d40ba2023-11-23T15:01:38ZengMDPI AGPharmaceuticals1424-82472022-01-011517210.3390/ph15010072Persimmon Leaves (<i>Diospyros kaki</i>) Extract Enhances the Viability of Human Corneal Endothelial Cells by Improving Na<sup>+</sup>-K<sup>+</sup>-ATPase ActivityRamsha Afzal0Hyung Bin Hwang1Department of Ophthalmology, Incheon St. Mary Hospital, College of Medicine, The Catholic University of Korea, 58 Dongsu-ro, Bupyeong-gu, Incheon 403-720, KoreaDepartment of Ophthalmology, Incheon St. Mary Hospital, College of Medicine, The Catholic University of Korea, 58 Dongsu-ro, Bupyeong-gu, Incheon 403-720, KoreaThe Na<sup>+</sup>/K<sup>+</sup>-ATPase, present in the basolateral membrane of human corneal endothelial cells (HCECs), is known to play an important role for corneal transparency. Na<sup>+</sup>/K<sup>+</sup>-ATPase dysfunction is one of the major causes of corneal decompensation. The ethanol extract of <i>Diospyros kaki</i> (EEDK) has been reported to increase corneal cell viability. Thus, we treated HCECs with EEDK and studied its effects on HCECs survival and Na<sup>+</sup>/K<sup>+</sup>-ATPase against cytotoxic drugs like staurosporine (ST) and ouabain (OU). Firstly, survival assays, (MTT assay and live dead-imaging) showed that decreased HCECs viability by ST and OU was significantly recovered by EEDK co-treatment. Secondly, Na<sup>+</sup>/K<sup>+</sup>-ATPase activity assays revealed that EEDK enhanced Na<sup>+</sup>/K<sup>+</sup>-ATPase enzymatic activity (* <i>p</i> < 0.01) with/without ST and OU. Finally, Na<sup>+</sup>/K<sup>+</sup>-ATPase expression analysis (Western Blot and confocal microscopy) demonstrated that EEDK treatment with/without ST and OU facilitates Na<sup>+</sup>/K<sup>+</sup>-ATPase expression in HCECs. Taken together, our findings led us to the conclusion that EEDK might aid HCECs survival in vitro by increasing the activity and expression of Na<sup>+</sup>/K<sup>+</sup>-ATPase enzyme. Since Na<sup>+</sup>/K<sup>+</sup>-ATPase activity is important to maintain cellular function of HCECs, we suggest that EEDK can be a potential effective agent against corneal edema and related corneal disorders.https://www.mdpi.com/1424-8247/15/1/72Na<sup>+</sup>/K<sup>+</sup>-ATPasehuman corneal endothelial cellsethanol extract of <i>Diospyros kaki</i>corneacell viabilityenzymatic activity |
| spellingShingle | Ramsha Afzal Hyung Bin Hwang Persimmon Leaves (<i>Diospyros kaki</i>) Extract Enhances the Viability of Human Corneal Endothelial Cells by Improving Na<sup>+</sup>-K<sup>+</sup>-ATPase Activity Pharmaceuticals Na<sup>+</sup>/K<sup>+</sup>-ATPase human corneal endothelial cells ethanol extract of <i>Diospyros kaki</i> cornea cell viability enzymatic activity |
| title | Persimmon Leaves (<i>Diospyros kaki</i>) Extract Enhances the Viability of Human Corneal Endothelial Cells by Improving Na<sup>+</sup>-K<sup>+</sup>-ATPase Activity |
| title_full | Persimmon Leaves (<i>Diospyros kaki</i>) Extract Enhances the Viability of Human Corneal Endothelial Cells by Improving Na<sup>+</sup>-K<sup>+</sup>-ATPase Activity |
| title_fullStr | Persimmon Leaves (<i>Diospyros kaki</i>) Extract Enhances the Viability of Human Corneal Endothelial Cells by Improving Na<sup>+</sup>-K<sup>+</sup>-ATPase Activity |
| title_full_unstemmed | Persimmon Leaves (<i>Diospyros kaki</i>) Extract Enhances the Viability of Human Corneal Endothelial Cells by Improving Na<sup>+</sup>-K<sup>+</sup>-ATPase Activity |
| title_short | Persimmon Leaves (<i>Diospyros kaki</i>) Extract Enhances the Viability of Human Corneal Endothelial Cells by Improving Na<sup>+</sup>-K<sup>+</sup>-ATPase Activity |
| title_sort | persimmon leaves i diospyros kaki i extract enhances the viability of human corneal endothelial cells by improving na sup sup k sup sup atpase activity |
| topic | Na<sup>+</sup>/K<sup>+</sup>-ATPase human corneal endothelial cells ethanol extract of <i>Diospyros kaki</i> cornea cell viability enzymatic activity |
| url | https://www.mdpi.com/1424-8247/15/1/72 |
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