Association of Lactoferrin and Toll-like Receptor 2 Genotypes with Mastitis and Milk Components in Vietnamese Holstein Cattle
Mastitis is one of the most widespread diseases in dairy cows and causes huge losses for the dairy industry. Molecular markers can be used for the quick diagnosis of mastitis infection, consequently reducing the loss caused by this disease. Lactoferrin (LTF) and Toll-like receptor 2 (TLR2) have been...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/8/379 |
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| author | Lan Doan Pham Nguyen Van Ba Le Quang Nam Phong Vuong Tuan Duy Ngoc Do |
| author_facet | Lan Doan Pham Nguyen Van Ba Le Quang Nam Phong Vuong Tuan Duy Ngoc Do |
| author_sort | Lan Doan Pham |
| collection | DOAJ |
| description | Mastitis is one of the most widespread diseases in dairy cows and causes huge losses for the dairy industry. Molecular markers can be used for the quick diagnosis of mastitis infection, consequently reducing the loss caused by this disease. Lactoferrin (LTF) and Toll-like receptor 2 (TLR2) have been suggested as candidate genes for mastitis; however, their associations with the mastitis incidence and milk components have not been reported in Vietnamese Holstein cows. This study examined the association of TLR2 and LTF polymorphisms with subclinical mastitis and milk components in the Holstein breed raised in Vietnam. Among 192 samples, we identified 44 mastitis-positive samples (22.92%). The mastitis significantly reduced the fat and lactose components in milk (<i>p</i> < 0.001) but increased the protein concentration in milk. A total of 94 (49%) and 98 (51%) cows had AA and AB genotypes for the LTF gene, respectively. No significant association was found between the LTF genotypes and the milk component traits or mastitis incidence (<i>p</i> > 0.05). The interaction between LTF and mastitis incidence was significantly associated with the protein percentage (<i>p</i> = 0.01). A total of 78, 76, and 38 cows had genotypes GG, GT, and TT for the TLR2 gene, respectively. TLR2 genotypes were not significantly associated with mastitis incidence (<i>p</i> > 0.05) but were significantly associated with pH value (<i>p</i> = 0.03). The interaction between TLR2 and mastitis incidence was significantly associated with the fat (<i>p</i> = 0.02) and protein percentage (<i>p</i> = 0.04). Further studies are required to confirm the roles of LTF and TFL2 in mastitis in the Holstein breed in Vietnam. |
| first_indexed | 2024-03-09T03:42:24Z |
| format | Article |
| id | doaj.art-547f2af92d4141babfb89eefed3f818f |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T03:42:24Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-547f2af92d4141babfb89eefed3f818f2023-12-03T14:38:04ZengMDPI AGVeterinary Sciences2306-73812022-07-019837910.3390/vetsci9080379Association of Lactoferrin and Toll-like Receptor 2 Genotypes with Mastitis and Milk Components in Vietnamese Holstein CattleLan Doan Pham0Nguyen Van Ba1Le Quang Nam2Phong Vuong Tuan3Duy Ngoc Do4Key Laboratory of Animal Cell Technology, National Institute of Animal Sciences, Thuyphuong, Tuliem, Hanoi 100000, VietnamKey Laboratory of Animal Cell Technology, National Institute of Animal Sciences, Thuyphuong, Tuliem, Hanoi 100000, VietnamKey Laboratory of Animal Cell Technology, National Institute of Animal Sciences, Thuyphuong, Tuliem, Hanoi 100000, VietnamBiodiversity Conservation and Tropical Diseases Research Institute, Hanoi 100000, VietnamDepartment of Animal Science and Aquaculture, Dalhousie University, Truro, NS B2N 5E3, CanadaMastitis is one of the most widespread diseases in dairy cows and causes huge losses for the dairy industry. Molecular markers can be used for the quick diagnosis of mastitis infection, consequently reducing the loss caused by this disease. Lactoferrin (LTF) and Toll-like receptor 2 (TLR2) have been suggested as candidate genes for mastitis; however, their associations with the mastitis incidence and milk components have not been reported in Vietnamese Holstein cows. This study examined the association of TLR2 and LTF polymorphisms with subclinical mastitis and milk components in the Holstein breed raised in Vietnam. Among 192 samples, we identified 44 mastitis-positive samples (22.92%). The mastitis significantly reduced the fat and lactose components in milk (<i>p</i> < 0.001) but increased the protein concentration in milk. A total of 94 (49%) and 98 (51%) cows had AA and AB genotypes for the LTF gene, respectively. No significant association was found between the LTF genotypes and the milk component traits or mastitis incidence (<i>p</i> > 0.05). The interaction between LTF and mastitis incidence was significantly associated with the protein percentage (<i>p</i> = 0.01). A total of 78, 76, and 38 cows had genotypes GG, GT, and TT for the TLR2 gene, respectively. TLR2 genotypes were not significantly associated with mastitis incidence (<i>p</i> > 0.05) but were significantly associated with pH value (<i>p</i> = 0.03). The interaction between TLR2 and mastitis incidence was significantly associated with the fat (<i>p</i> = 0.02) and protein percentage (<i>p</i> = 0.04). Further studies are required to confirm the roles of LTF and TFL2 in mastitis in the Holstein breed in Vietnam.https://www.mdpi.com/2306-7381/9/8/379mastitisTLR2LTFmilk compositionHolstein |
| spellingShingle | Lan Doan Pham Nguyen Van Ba Le Quang Nam Phong Vuong Tuan Duy Ngoc Do Association of Lactoferrin and Toll-like Receptor 2 Genotypes with Mastitis and Milk Components in Vietnamese Holstein Cattle Veterinary Sciences mastitis TLR2 LTF milk composition Holstein |
| title | Association of Lactoferrin and Toll-like Receptor 2 Genotypes with Mastitis and Milk Components in Vietnamese Holstein Cattle |
| title_full | Association of Lactoferrin and Toll-like Receptor 2 Genotypes with Mastitis and Milk Components in Vietnamese Holstein Cattle |
| title_fullStr | Association of Lactoferrin and Toll-like Receptor 2 Genotypes with Mastitis and Milk Components in Vietnamese Holstein Cattle |
| title_full_unstemmed | Association of Lactoferrin and Toll-like Receptor 2 Genotypes with Mastitis and Milk Components in Vietnamese Holstein Cattle |
| title_short | Association of Lactoferrin and Toll-like Receptor 2 Genotypes with Mastitis and Milk Components in Vietnamese Holstein Cattle |
| title_sort | association of lactoferrin and toll like receptor 2 genotypes with mastitis and milk components in vietnamese holstein cattle |
| topic | mastitis TLR2 LTF milk composition Holstein |
| url | https://www.mdpi.com/2306-7381/9/8/379 |
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