A bacteriophage cocktail delivered in feed significantly reduced Salmonella colonization in challenged broiler chickens
ABSTRACTNontyphoidal Salmonella spp. are a leading cause of human gastrointestinal infections and are commonly transmitted via the consumption of contaminated meat. To limit the spread of Salmonella and other food-borne pathogens in the food chain, bacteriophage (phage) therapy could be used during...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2023.2217947 |
| _version_ | 1797652795942240256 |
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| author | Anisha M. Thanki Steven Hooton Natasha Whenham Michael G. Salter Mike R. Bedford Helen V. Masey O'Neill Martha R. J. Clokie |
| author_facet | Anisha M. Thanki Steven Hooton Natasha Whenham Michael G. Salter Mike R. Bedford Helen V. Masey O'Neill Martha R. J. Clokie |
| author_sort | Anisha M. Thanki |
| collection | DOAJ |
| description | ABSTRACTNontyphoidal Salmonella spp. are a leading cause of human gastrointestinal infections and are commonly transmitted via the consumption of contaminated meat. To limit the spread of Salmonella and other food-borne pathogens in the food chain, bacteriophage (phage) therapy could be used during rearing or pre-harvest stages of animal production. This study was conducted to determine if a phage cocktail delivered in feed is capable of reducing Salmonella colonization in experimentally challenged chickens and to determine the optimal phage dose. A total of 672 broilers were divided into six treatment groups T1 (no phage diet and unchallenged); T2 (phage diet 106 PFU/day); T3 (challenged group); T4 (phage diet 105 PFU/day and challenged); T5 (phage diet 106 PFU/day and challenged); and T6 (phage diet 107 PFU/day and challenged). The liquid phage cocktail was added to mash diet with ad libitum access available throughout the study. By day 42 (the concluding day of the study), no Salmonella was detected in faecal samples collected from group T4. Salmonella was isolated from a small number of pens in groups T5 (3/16) and T6 (2/16) at ∼4 × 102 CFU/g. In comparison, Salmonella was isolated from 7/16 pens in T3 at ∼3 × 104 CFU/g. Phage treatment at all three doses had a positive impact on growth performance in challenged birds with increased weight gains in comparison to challenged birds with no phage diet. We showed delivering phages via feed was effective at reducing Salmonella colonization in chickens and our study highlights phages offer a promising tool to target bacterial infections in poultry. |
| first_indexed | 2024-03-11T16:35:09Z |
| format | Article |
| id | doaj.art-54829f28f03f450d84d1ada5473d814d |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-03-11T16:35:09Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-54829f28f03f450d84d1ada5473d814d2023-10-23T17:36:54ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512023-12-0112110.1080/22221751.2023.2217947A bacteriophage cocktail delivered in feed significantly reduced Salmonella colonization in challenged broiler chickensAnisha M. Thanki0Steven Hooton1Natasha Whenham2Michael G. Salter3Mike R. Bedford4Helen V. Masey O'Neill5Martha R. J. Clokie6Department of Genetics and Genome Biology, University of Leicester, Leicester, UKDepartment of Genetics and Genome Biology, University of Leicester, Leicester, UKAb Agri, Innovation Way, Peterborough Business Park, Peterborough, UKAb Agri, Innovation Way, Peterborough Business Park, Peterborough, UKAb Vista, Woodstock Court, Marlborough, UKAb Agri, Innovation Way, Peterborough Business Park, Peterborough, UKDepartment of Genetics and Genome Biology, University of Leicester, Leicester, UKABSTRACTNontyphoidal Salmonella spp. are a leading cause of human gastrointestinal infections and are commonly transmitted via the consumption of contaminated meat. To limit the spread of Salmonella and other food-borne pathogens in the food chain, bacteriophage (phage) therapy could be used during rearing or pre-harvest stages of animal production. This study was conducted to determine if a phage cocktail delivered in feed is capable of reducing Salmonella colonization in experimentally challenged chickens and to determine the optimal phage dose. A total of 672 broilers were divided into six treatment groups T1 (no phage diet and unchallenged); T2 (phage diet 106 PFU/day); T3 (challenged group); T4 (phage diet 105 PFU/day and challenged); T5 (phage diet 106 PFU/day and challenged); and T6 (phage diet 107 PFU/day and challenged). The liquid phage cocktail was added to mash diet with ad libitum access available throughout the study. By day 42 (the concluding day of the study), no Salmonella was detected in faecal samples collected from group T4. Salmonella was isolated from a small number of pens in groups T5 (3/16) and T6 (2/16) at ∼4 × 102 CFU/g. In comparison, Salmonella was isolated from 7/16 pens in T3 at ∼3 × 104 CFU/g. Phage treatment at all three doses had a positive impact on growth performance in challenged birds with increased weight gains in comparison to challenged birds with no phage diet. We showed delivering phages via feed was effective at reducing Salmonella colonization in chickens and our study highlights phages offer a promising tool to target bacterial infections in poultry.https://www.tandfonline.com/doi/10.1080/22221751.2023.2217947Salmonellaphagesphage therapyantimicrobial resistancepoultry |
| spellingShingle | Anisha M. Thanki Steven Hooton Natasha Whenham Michael G. Salter Mike R. Bedford Helen V. Masey O'Neill Martha R. J. Clokie A bacteriophage cocktail delivered in feed significantly reduced Salmonella colonization in challenged broiler chickens Emerging Microbes and Infections Salmonella phages phage therapy antimicrobial resistance poultry |
| title | A bacteriophage cocktail delivered in feed significantly reduced Salmonella colonization in challenged broiler chickens |
| title_full | A bacteriophage cocktail delivered in feed significantly reduced Salmonella colonization in challenged broiler chickens |
| title_fullStr | A bacteriophage cocktail delivered in feed significantly reduced Salmonella colonization in challenged broiler chickens |
| title_full_unstemmed | A bacteriophage cocktail delivered in feed significantly reduced Salmonella colonization in challenged broiler chickens |
| title_short | A bacteriophage cocktail delivered in feed significantly reduced Salmonella colonization in challenged broiler chickens |
| title_sort | bacteriophage cocktail delivered in feed significantly reduced salmonella colonization in challenged broiler chickens |
| topic | Salmonella phages phage therapy antimicrobial resistance poultry |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2023.2217947 |
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