Emergence of uniform linearly-arranged micro-droplets entrapping DNA and living cells through water/water phase-separation
Abstract Living cells maintain their lives through self-organization in an environment crowded with a rich variety of biological species. Recently, it was found that micro-droplets containing biomacromolecules, which vary widely in size, are generated accompanied by water/water phase-separation by s...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-02990-w |
| _version_ | 1818388619357847552 |
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| author | Mayu Shono Ritsuki Ito Fumika Fujita Hiroki Sakuta Kenichi Yoshikawa |
| author_facet | Mayu Shono Ritsuki Ito Fumika Fujita Hiroki Sakuta Kenichi Yoshikawa |
| author_sort | Mayu Shono |
| collection | DOAJ |
| description | Abstract Living cells maintain their lives through self-organization in an environment crowded with a rich variety of biological species. Recently, it was found that micro-droplets containing biomacromolecules, which vary widely in size, are generated accompanied by water/water phase-separation by simple mechanical mixing of an aqueous solution with binary polymers. Here, we report that cell-sized droplets of nearly the same size are generated as a linear array within a glass capillary upon the introduction of a binary polymer solution of polyethylene glycol (PEG) and dextran (DEX). Interestingly, when DNA molecules are added to the polymer solution, stable droplets entrapping DNA molecules are obtained. Similarly, living cells are entrapped spontaneously for the linearly-arranged cell-sized droplets. This simple method for generating micro-droplets entrapping DNA and also living cells is expected to stimulate further study on the self-construction of protocells and micro organoids. |
| first_indexed | 2024-12-14T04:28:43Z |
| format | Article |
| id | doaj.art-54832692201a47d9806fa942c5b3c852 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T04:28:43Z |
| publishDate | 2021-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-54832692201a47d9806fa942c5b3c8522022-12-21T23:17:08ZengNature PortfolioScientific Reports2045-23222021-12-011111910.1038/s41598-021-02990-wEmergence of uniform linearly-arranged micro-droplets entrapping DNA and living cells through water/water phase-separationMayu Shono0Ritsuki Ito1Fumika Fujita2Hiroki Sakuta3Kenichi Yoshikawa4Department of Chemical Engineering and Materials Science, Doshisha UniversityFaculty of Life and Medical Sciences, Doshisha UniversityFaculty of Life and Medical Sciences, Doshisha UniversityFaculty of Life and Medical Sciences, Doshisha UniversityFaculty of Life and Medical Sciences, Doshisha UniversityAbstract Living cells maintain their lives through self-organization in an environment crowded with a rich variety of biological species. Recently, it was found that micro-droplets containing biomacromolecules, which vary widely in size, are generated accompanied by water/water phase-separation by simple mechanical mixing of an aqueous solution with binary polymers. Here, we report that cell-sized droplets of nearly the same size are generated as a linear array within a glass capillary upon the introduction of a binary polymer solution of polyethylene glycol (PEG) and dextran (DEX). Interestingly, when DNA molecules are added to the polymer solution, stable droplets entrapping DNA molecules are obtained. Similarly, living cells are entrapped spontaneously for the linearly-arranged cell-sized droplets. This simple method for generating micro-droplets entrapping DNA and also living cells is expected to stimulate further study on the self-construction of protocells and micro organoids.https://doi.org/10.1038/s41598-021-02990-w |
| spellingShingle | Mayu Shono Ritsuki Ito Fumika Fujita Hiroki Sakuta Kenichi Yoshikawa Emergence of uniform linearly-arranged micro-droplets entrapping DNA and living cells through water/water phase-separation Scientific Reports |
| title | Emergence of uniform linearly-arranged micro-droplets entrapping DNA and living cells through water/water phase-separation |
| title_full | Emergence of uniform linearly-arranged micro-droplets entrapping DNA and living cells through water/water phase-separation |
| title_fullStr | Emergence of uniform linearly-arranged micro-droplets entrapping DNA and living cells through water/water phase-separation |
| title_full_unstemmed | Emergence of uniform linearly-arranged micro-droplets entrapping DNA and living cells through water/water phase-separation |
| title_short | Emergence of uniform linearly-arranged micro-droplets entrapping DNA and living cells through water/water phase-separation |
| title_sort | emergence of uniform linearly arranged micro droplets entrapping dna and living cells through water water phase separation |
| url | https://doi.org/10.1038/s41598-021-02990-w |
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