Autologous G-CSF-Mobilized Peripheral Blood CD34 Cell Therapy for Diabetic Patients with Chronic Nonhealing Ulcer
Recently, animal studies have demonstrated the efficacy of endothelial progenitor cell (EPC) therapy for diabetic wound healing. Based on these preclinical studies, we performed a prospective clinical trial phase I/IIa study of autologous G-CSF-mobilized peripheral blood (PB) CD34 + cell transplanta...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2014-03-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368912X658007 |
| _version_ | 1811328075935252480 |
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| author | Rica Tanaka M.D., Ph.D. Haruchika Masuda Shunichi Kato Kotaro Imagawa Kazuo Kanabuchi Chie Nakashioya Fumiaki Yoshiba Tsuyoshi Fukui Rie Ito Michiru Kobori Mika Wada Takayuki Asahara Muneo Miyasaka |
| author_facet | Rica Tanaka M.D., Ph.D. Haruchika Masuda Shunichi Kato Kotaro Imagawa Kazuo Kanabuchi Chie Nakashioya Fumiaki Yoshiba Tsuyoshi Fukui Rie Ito Michiru Kobori Mika Wada Takayuki Asahara Muneo Miyasaka |
| author_sort | Rica Tanaka M.D., Ph.D. |
| collection | DOAJ |
| description | Recently, animal studies have demonstrated the efficacy of endothelial progenitor cell (EPC) therapy for diabetic wound healing. Based on these preclinical studies, we performed a prospective clinical trial phase I/IIa study of autologous G-CSF-mobilized peripheral blood (PB) CD34 + cell transplantation for nonhealing diabetic foot patients. Diabetic patients with nonhealing foot ulcers were treated with 2 × 10 7 cells of G-CSF-mobilized PB CD34 + cells as EPC-enriched population. Safety and efficacy (wound closure and vascular perfusion) were evaluated 12 weeks posttherapy and further followed for complete wound closure and recurrence. A total of five patients were enrolled. Although minor amputation and recurrence were seen in three out of five patients, no death, other serious adverse events, or major amputation was seen following transplantation. Complete wound closure was observed at an average of 18 weeks with increased vascular perfusion in all patients. The outcomes of this prospective clinical study indicate the safety and feasibility of CD34 + cell therapy in patients with diabetic nonhealing wounds. |
| first_indexed | 2024-04-13T15:18:47Z |
| format | Article |
| id | doaj.art-54834e497cee40b9a357c2ee50f44c9f |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-04-13T15:18:47Z |
| publishDate | 2014-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-54834e497cee40b9a357c2ee50f44c9f2022-12-22T02:41:44ZengSAGE PublishingCell Transplantation0963-68971555-38922014-03-012310.3727/096368912X658007Autologous G-CSF-Mobilized Peripheral Blood CD34 Cell Therapy for Diabetic Patients with Chronic Nonhealing UlcerRica Tanaka M.D., Ph.D.0Haruchika Masuda1Shunichi Kato2Kotaro Imagawa3Kazuo Kanabuchi4Chie Nakashioya5Fumiaki Yoshiba6Tsuyoshi Fukui7Rie Ito8Michiru Kobori9Mika Wada10Takayuki Asahara11Muneo Miyasaka12Department of Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, JapanDepartment of Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, JapanDepartment of Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, JapanDepartment of Plastic and Reconstructive Surgery, Tokai University School of Medicine, Isehara, Kanagawa, JapanDepartment of Cardiovascular Surgery, Tokai University School of Medicine, Isehara, Kanagawa, JapanDepartment of Blood Transfusion Service, Cell Transplantation and Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, JapanDepartment of Blood Transfusion Service, Cell Transplantation and Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, JapanDepartment of Plastic and Reconstructive Surgery, Tokai University School of Medicine, Isehara, Kanagawa, JapanDepartment of Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, JapanDepartment of Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, JapanDepartment of Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, JapanDepartment of Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, JapanDepartment of Plastic and Reconstructive Surgery, Tokai University School of Medicine, Isehara, Kanagawa, JapanRecently, animal studies have demonstrated the efficacy of endothelial progenitor cell (EPC) therapy for diabetic wound healing. Based on these preclinical studies, we performed a prospective clinical trial phase I/IIa study of autologous G-CSF-mobilized peripheral blood (PB) CD34 + cell transplantation for nonhealing diabetic foot patients. Diabetic patients with nonhealing foot ulcers were treated with 2 × 10 7 cells of G-CSF-mobilized PB CD34 + cells as EPC-enriched population. Safety and efficacy (wound closure and vascular perfusion) were evaluated 12 weeks posttherapy and further followed for complete wound closure and recurrence. A total of five patients were enrolled. Although minor amputation and recurrence were seen in three out of five patients, no death, other serious adverse events, or major amputation was seen following transplantation. Complete wound closure was observed at an average of 18 weeks with increased vascular perfusion in all patients. The outcomes of this prospective clinical study indicate the safety and feasibility of CD34 + cell therapy in patients with diabetic nonhealing wounds.https://doi.org/10.3727/096368912X658007 |
| spellingShingle | Rica Tanaka M.D., Ph.D. Haruchika Masuda Shunichi Kato Kotaro Imagawa Kazuo Kanabuchi Chie Nakashioya Fumiaki Yoshiba Tsuyoshi Fukui Rie Ito Michiru Kobori Mika Wada Takayuki Asahara Muneo Miyasaka Autologous G-CSF-Mobilized Peripheral Blood CD34 Cell Therapy for Diabetic Patients with Chronic Nonhealing Ulcer Cell Transplantation |
| title | Autologous G-CSF-Mobilized Peripheral Blood CD34 Cell Therapy for Diabetic Patients with Chronic Nonhealing Ulcer |
| title_full | Autologous G-CSF-Mobilized Peripheral Blood CD34 Cell Therapy for Diabetic Patients with Chronic Nonhealing Ulcer |
| title_fullStr | Autologous G-CSF-Mobilized Peripheral Blood CD34 Cell Therapy for Diabetic Patients with Chronic Nonhealing Ulcer |
| title_full_unstemmed | Autologous G-CSF-Mobilized Peripheral Blood CD34 Cell Therapy for Diabetic Patients with Chronic Nonhealing Ulcer |
| title_short | Autologous G-CSF-Mobilized Peripheral Blood CD34 Cell Therapy for Diabetic Patients with Chronic Nonhealing Ulcer |
| title_sort | autologous g csf mobilized peripheral blood cd34 cell therapy for diabetic patients with chronic nonhealing ulcer |
| url | https://doi.org/10.3727/096368912X658007 |
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