Expression of <i>ZjPSY</i>, a Phytoene Synthase Gene from <i>Zoysia japonica</i> Affects Plant Height and Photosynthetic Pigment Contents
Phytoene synthase (PSY) is a key limiting enzyme in the carotenoid biosynthesis pathway for regulating phytoene synthesis. In this study, <i>ZjPSY</i> was isolated and identified from <i>Zoysia japonica</i>, an important lawn grass species. <i>ZjPSY</i> cDNA was 1...
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MDPI AG
2022-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/3/395 |
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| author | Di Dong Yuhong Zhao Ke Teng Penghui Tan Zhuocheng Liu Zhuoxiong Yang Liebao Han Yuehui Chao |
| author_facet | Di Dong Yuhong Zhao Ke Teng Penghui Tan Zhuocheng Liu Zhuoxiong Yang Liebao Han Yuehui Chao |
| author_sort | Di Dong |
| collection | DOAJ |
| description | Phytoene synthase (PSY) is a key limiting enzyme in the carotenoid biosynthesis pathway for regulating phytoene synthesis. In this study, <i>ZjPSY</i> was isolated and identified from <i>Zoysia japonica</i>, an important lawn grass species. <i>ZjPSY</i> cDNA was 1230 bp in length, corresponding to 409 amino acids. <i>ZjPSY</i> showed higher expression in young leaves and was downregulated after GA<sub>3</sub>, ABA, SA, and MeJA treatments, exhibiting a sensitivity to plant hormones. Regulatory elements of light and plant hormone were found in the upstream of <i>ZjPSY</i> CDS. Expression of <i>ZjPSY</i> in <i>Arabidopsis thaliana</i> protein led to carotenoid accumulation and altered expression of genes involved in the carotenoid pathway. Under no-treatment condition, salt treatment, and drought treatment, transgenic plants exhibited yellowing, dwarfing phenotypes. The carotenoid content of transgenic plants was significantly higher than that of wild-type under salt stress and no-treatment condition. Yeast two-hybrid screening identified a novel interacting partner <i>ZjJ2</i> (<i>DNAJ homologue 2</i>), which encodes heat-shock protein 40 (HSP40). Taken together, this study suggested that ZjPSY may affect plant height and play an important role in carotenoid synthesis. These results broadened the understanding of carotenoid synthesis pathways and laid a foundation for the exploration and utilization of the <i>PSY</i> gene. |
| first_indexed | 2024-03-09T23:18:28Z |
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| last_indexed | 2024-03-09T23:18:28Z |
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| spelling | doaj.art-54836fc928e34b9aac53a085ace2085f2023-11-23T17:31:17ZengMDPI AGPlants2223-77472022-01-0111339510.3390/plants11030395Expression of <i>ZjPSY</i>, a Phytoene Synthase Gene from <i>Zoysia japonica</i> Affects Plant Height and Photosynthetic Pigment ContentsDi Dong0Yuhong Zhao1Ke Teng2Penghui Tan3Zhuocheng Liu4Zhuoxiong Yang5Liebao Han6Yuehui Chao7School of Grassland Science, Beijing Forestry University, Beijing 100083, ChinaAnimal Science College, Tibet Agriculture & Animal Husbandry University, Nyingchi 860000, ChinaSchool of Grassland Science, Beijing Forestry University, Beijing 100083, ChinaBeijing Chaoyang Foreign Language School, Beijing 100101, ChinaSchool of Grassland Science, Beijing Forestry University, Beijing 100083, ChinaSchool of Grassland Science, Beijing Forestry University, Beijing 100083, ChinaSchool of Grassland Science, Beijing Forestry University, Beijing 100083, ChinaSchool of Grassland Science, Beijing Forestry University, Beijing 100083, ChinaPhytoene synthase (PSY) is a key limiting enzyme in the carotenoid biosynthesis pathway for regulating phytoene synthesis. In this study, <i>ZjPSY</i> was isolated and identified from <i>Zoysia japonica</i>, an important lawn grass species. <i>ZjPSY</i> cDNA was 1230 bp in length, corresponding to 409 amino acids. <i>ZjPSY</i> showed higher expression in young leaves and was downregulated after GA<sub>3</sub>, ABA, SA, and MeJA treatments, exhibiting a sensitivity to plant hormones. Regulatory elements of light and plant hormone were found in the upstream of <i>ZjPSY</i> CDS. Expression of <i>ZjPSY</i> in <i>Arabidopsis thaliana</i> protein led to carotenoid accumulation and altered expression of genes involved in the carotenoid pathway. Under no-treatment condition, salt treatment, and drought treatment, transgenic plants exhibited yellowing, dwarfing phenotypes. The carotenoid content of transgenic plants was significantly higher than that of wild-type under salt stress and no-treatment condition. Yeast two-hybrid screening identified a novel interacting partner <i>ZjJ2</i> (<i>DNAJ homologue 2</i>), which encodes heat-shock protein 40 (HSP40). Taken together, this study suggested that ZjPSY may affect plant height and play an important role in carotenoid synthesis. These results broadened the understanding of carotenoid synthesis pathways and laid a foundation for the exploration and utilization of the <i>PSY</i> gene.https://www.mdpi.com/2223-7747/11/3/395carotenoidsdwarfing<i>Zoysia japonica</i> |
| spellingShingle | Di Dong Yuhong Zhao Ke Teng Penghui Tan Zhuocheng Liu Zhuoxiong Yang Liebao Han Yuehui Chao Expression of <i>ZjPSY</i>, a Phytoene Synthase Gene from <i>Zoysia japonica</i> Affects Plant Height and Photosynthetic Pigment Contents Plants carotenoids dwarfing <i>Zoysia japonica</i> |
| title | Expression of <i>ZjPSY</i>, a Phytoene Synthase Gene from <i>Zoysia japonica</i> Affects Plant Height and Photosynthetic Pigment Contents |
| title_full | Expression of <i>ZjPSY</i>, a Phytoene Synthase Gene from <i>Zoysia japonica</i> Affects Plant Height and Photosynthetic Pigment Contents |
| title_fullStr | Expression of <i>ZjPSY</i>, a Phytoene Synthase Gene from <i>Zoysia japonica</i> Affects Plant Height and Photosynthetic Pigment Contents |
| title_full_unstemmed | Expression of <i>ZjPSY</i>, a Phytoene Synthase Gene from <i>Zoysia japonica</i> Affects Plant Height and Photosynthetic Pigment Contents |
| title_short | Expression of <i>ZjPSY</i>, a Phytoene Synthase Gene from <i>Zoysia japonica</i> Affects Plant Height and Photosynthetic Pigment Contents |
| title_sort | expression of i zjpsy i a phytoene synthase gene from i zoysia japonica i affects plant height and photosynthetic pigment contents |
| topic | carotenoids dwarfing <i>Zoysia japonica</i> |
| url | https://www.mdpi.com/2223-7747/11/3/395 |
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