Spodoptera frugiperda transcriptional response to infestation by Steinernema carpocapsae
Abstract Steinernema carpocapsae is an entomopathogenic nematode (EPN) used in biological control of agricultural pest insects. It enters the hemocoel of its host via the intestinal tract and releases its symbiotic bacterium Xenorhabdus nematophila. In order to improve our knowledge about the physio...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2019-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-019-49410-8 |
| _version_ | 1818996420346118144 |
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| author | Louise Huot Simon George Pierre-Alain Girard Dany Severac Nicolas Nègre Bernard Duvic |
| author_facet | Louise Huot Simon George Pierre-Alain Girard Dany Severac Nicolas Nègre Bernard Duvic |
| author_sort | Louise Huot |
| collection | DOAJ |
| description | Abstract Steinernema carpocapsae is an entomopathogenic nematode (EPN) used in biological control of agricultural pest insects. It enters the hemocoel of its host via the intestinal tract and releases its symbiotic bacterium Xenorhabdus nematophila. In order to improve our knowledge about the physiological responses of its different hosts, we examined the transcriptional responses to EPN infestation of the fat body, the hemocytes and the midgut in the lepidopteran pest Spodoptera frugiperda. The tissues poorly respond to the infestation at an early time post-infestation of 8 h with only 5 genes differentially expressed in the fat body of the caterpillars. Strong transcriptional responses are observed at a later time point of 15 h post-infestation in all three tissues. Few genes are differentially expressed in the midgut but tissue-specific panels of induced metalloprotease inhibitors, immune receptors and antimicrobial peptides together with several uncharacterized genes are up-regulated in the fat body and the hemocytes. Among the most up-regulated genes, we identified new potential immune effectors, unique to Lepidoptera, which show homology with bacterial genes of unknown function. Altogether, these results pave the way for further functional studies of the responsive genes’ involvement in the interaction with the EPN. |
| first_indexed | 2024-12-20T21:29:27Z |
| format | Article |
| id | doaj.art-548374f4997747c79dafa34d8f89a9ec |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T21:29:27Z |
| publishDate | 2019-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-548374f4997747c79dafa34d8f89a9ec2022-12-21T19:26:04ZengNature PortfolioScientific Reports2045-23222019-09-019111310.1038/s41598-019-49410-8Spodoptera frugiperda transcriptional response to infestation by Steinernema carpocapsaeLouise Huot0Simon George1Pierre-Alain Girard2Dany Severac3Nicolas Nègre4Bernard Duvic5DGIMI, Univ Montpellier, INRADGIMI, Univ Montpellier, INRADGIMI, Univ Montpellier, INRAMGX, Univ Montpellier, CNRS, INSERMDGIMI, Univ Montpellier, INRADGIMI, Univ Montpellier, INRAAbstract Steinernema carpocapsae is an entomopathogenic nematode (EPN) used in biological control of agricultural pest insects. It enters the hemocoel of its host via the intestinal tract and releases its symbiotic bacterium Xenorhabdus nematophila. In order to improve our knowledge about the physiological responses of its different hosts, we examined the transcriptional responses to EPN infestation of the fat body, the hemocytes and the midgut in the lepidopteran pest Spodoptera frugiperda. The tissues poorly respond to the infestation at an early time post-infestation of 8 h with only 5 genes differentially expressed in the fat body of the caterpillars. Strong transcriptional responses are observed at a later time point of 15 h post-infestation in all three tissues. Few genes are differentially expressed in the midgut but tissue-specific panels of induced metalloprotease inhibitors, immune receptors and antimicrobial peptides together with several uncharacterized genes are up-regulated in the fat body and the hemocytes. Among the most up-regulated genes, we identified new potential immune effectors, unique to Lepidoptera, which show homology with bacterial genes of unknown function. Altogether, these results pave the way for further functional studies of the responsive genes’ involvement in the interaction with the EPN.https://doi.org/10.1038/s41598-019-49410-8 |
| spellingShingle | Louise Huot Simon George Pierre-Alain Girard Dany Severac Nicolas Nègre Bernard Duvic Spodoptera frugiperda transcriptional response to infestation by Steinernema carpocapsae Scientific Reports |
| title | Spodoptera frugiperda transcriptional response to infestation by Steinernema carpocapsae |
| title_full | Spodoptera frugiperda transcriptional response to infestation by Steinernema carpocapsae |
| title_fullStr | Spodoptera frugiperda transcriptional response to infestation by Steinernema carpocapsae |
| title_full_unstemmed | Spodoptera frugiperda transcriptional response to infestation by Steinernema carpocapsae |
| title_short | Spodoptera frugiperda transcriptional response to infestation by Steinernema carpocapsae |
| title_sort | spodoptera frugiperda transcriptional response to infestation by steinernema carpocapsae |
| url | https://doi.org/10.1038/s41598-019-49410-8 |
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