Cytoskeleton structure and total methylation of mouse cardiac and lung tissue during space flight.
The purpose of this work was to evaluate the protein and mRNA expression levels of multiple cytoskeletal proteins in the cardiac and lung tissue of mice that were euthanized onboard the United States Orbital Segment of the International Space Station 37 days after the start of the SpaceX-4 mission (...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5955502?pdf=render |
| _version_ | 1819211135195283456 |
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| author | Irina V Ogneva Sergey S Loktev Vladimir N Sychev |
| author_facet | Irina V Ogneva Sergey S Loktev Vladimir N Sychev |
| author_sort | Irina V Ogneva |
| collection | DOAJ |
| description | The purpose of this work was to evaluate the protein and mRNA expression levels of multiple cytoskeletal proteins in the cardiac and lung tissue of mice that were euthanized onboard the United States Orbital Segment of the International Space Station 37 days after the start of the SpaceX-4 mission (September 2014, USA). The results showed no changes in the cytoskeletal protein content in the cardiac and lung tissue of the mice, but there were significant changes in the mRNA expression levels of the associated genes, which may be due to an increase in total genome methylation. The mRNA expression levels of DNA methylases, the cytosine demethylases Tet1 and Tet3, histone acetylase and histone deacetylase did not change, and the mRNA expression level of cytosine demethylase Tet2 was significantly decreased. |
| first_indexed | 2024-12-23T06:22:15Z |
| format | Article |
| id | doaj.art-5485090bad664a7ca250fa33f62bb9db |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T06:22:15Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5485090bad664a7ca250fa33f62bb9db2022-12-21T17:57:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01135e019264310.1371/journal.pone.0192643Cytoskeleton structure and total methylation of mouse cardiac and lung tissue during space flight.Irina V OgnevaSergey S LoktevVladimir N SychevThe purpose of this work was to evaluate the protein and mRNA expression levels of multiple cytoskeletal proteins in the cardiac and lung tissue of mice that were euthanized onboard the United States Orbital Segment of the International Space Station 37 days after the start of the SpaceX-4 mission (September 2014, USA). The results showed no changes in the cytoskeletal protein content in the cardiac and lung tissue of the mice, but there were significant changes in the mRNA expression levels of the associated genes, which may be due to an increase in total genome methylation. The mRNA expression levels of DNA methylases, the cytosine demethylases Tet1 and Tet3, histone acetylase and histone deacetylase did not change, and the mRNA expression level of cytosine demethylase Tet2 was significantly decreased.http://europepmc.org/articles/PMC5955502?pdf=render |
| spellingShingle | Irina V Ogneva Sergey S Loktev Vladimir N Sychev Cytoskeleton structure and total methylation of mouse cardiac and lung tissue during space flight. PLoS ONE |
| title | Cytoskeleton structure and total methylation of mouse cardiac and lung tissue during space flight. |
| title_full | Cytoskeleton structure and total methylation of mouse cardiac and lung tissue during space flight. |
| title_fullStr | Cytoskeleton structure and total methylation of mouse cardiac and lung tissue during space flight. |
| title_full_unstemmed | Cytoskeleton structure and total methylation of mouse cardiac and lung tissue during space flight. |
| title_short | Cytoskeleton structure and total methylation of mouse cardiac and lung tissue during space flight. |
| title_sort | cytoskeleton structure and total methylation of mouse cardiac and lung tissue during space flight |
| url | http://europepmc.org/articles/PMC5955502?pdf=render |
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