Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection
Nanoparticles derived from the elongated flexuous capsids of <i>Turnip mosaic virus</i> (TuMV) have been shown to be efficient tools for antibody sensing with a very high sensitivity if adequately functionalized with the corresponding epitopes. Taking advantage of this possibility, TuMV...
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| Format: | Article |
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MDPI AG
2019-10-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/9/10/1438 |
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| author | Carmen Yuste-Calvo Mercedes López-Santalla Lucía Zurita César F. Cruz-Fernández Flora Sánchez Marina I. Garín Fernando Ponz |
| author_facet | Carmen Yuste-Calvo Mercedes López-Santalla Lucía Zurita César F. Cruz-Fernández Flora Sánchez Marina I. Garín Fernando Ponz |
| author_sort | Carmen Yuste-Calvo |
| collection | DOAJ |
| description | Nanoparticles derived from the elongated flexuous capsids of <i>Turnip mosaic virus</i> (TuMV) have been shown to be efficient tools for antibody sensing with a very high sensitivity if adequately functionalized with the corresponding epitopes. Taking advantage of this possibility, TuMV virus-like particles (VLPs) have been genetically derivatized with a peptide from the chaperonin Hsp60, a protein described to be involved in inflammation processes and autoimmune diseases. Antibodies against the peptide have been previously shown to have a diagnostic value in at least one autoimmune disease, multiple sclerosis. The functionalized Hsp60-VLPs showed their significant increase in sensing potency when compared to monoclonal antibody detection of the peptide in a conventional immunoassay. Additionally, the developed Hsp60-VLPs allowed the detection of autoantibodies against the Hsp60 peptide in an in vivo mouse model of dextran sodium sulfate (DSS)-induced colitis. The detection of minute amounts of the autoantibodies allowed us to perform the analysis of their evolution during the progression of the disease. The anti-Hsp60 autoantibody levels in the sera of the inflamed mice went down during the induction phase of the disease. Increased levels of the anti-HSP60 autoantibodies were detected during the resolution phase of the disease. An extension of a previously proposed model for the involvement of Hsp60 in inflammatory processes is considered, incorporating a role for Hsp60 autoantibodies. This, and related models, can now be experimentally tested thanks to the autoantibody detection hypersensitivity provided by the functionalized VLPs. |
| first_indexed | 2024-12-23T19:48:10Z |
| format | Article |
| id | doaj.art-548aef35fd54488dbd97202df096a17d |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-23T19:48:10Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-548aef35fd54488dbd97202df096a17d2022-12-21T17:33:27ZengMDPI AGNanomaterials2079-49912019-10-01910143810.3390/nano9101438nano9101438Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody DetectionCarmen Yuste-Calvo0Mercedes López-Santalla1Lucía Zurita2César F. Cruz-Fernández3Flora Sánchez4Marina I. Garín5Fernando Ponz6Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (CBGP, UPM-INIA), Campus Montegancedo, Autopista M-40, km 38. Pozuelo de Alarcón, 28223 Madrid, SpainDivision of Hematopoietic Innovative Therapies, Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT) and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBER-ER), 28040 Madrid, SpainCentro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (CBGP, UPM-INIA), Campus Montegancedo, Autopista M-40, km 38. Pozuelo de Alarcón, 28223 Madrid, SpainCentro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (CBGP, UPM-INIA), Campus Montegancedo, Autopista M-40, km 38. Pozuelo de Alarcón, 28223 Madrid, SpainCentro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (CBGP, UPM-INIA), Campus Montegancedo, Autopista M-40, km 38. Pozuelo de Alarcón, 28223 Madrid, SpainDivision of Hematopoietic Innovative Therapies, Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT) and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBER-ER), 28040 Madrid, SpainCentro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (CBGP, UPM-INIA), Campus Montegancedo, Autopista M-40, km 38. Pozuelo de Alarcón, 28223 Madrid, SpainNanoparticles derived from the elongated flexuous capsids of <i>Turnip mosaic virus</i> (TuMV) have been shown to be efficient tools for antibody sensing with a very high sensitivity if adequately functionalized with the corresponding epitopes. Taking advantage of this possibility, TuMV virus-like particles (VLPs) have been genetically derivatized with a peptide from the chaperonin Hsp60, a protein described to be involved in inflammation processes and autoimmune diseases. Antibodies against the peptide have been previously shown to have a diagnostic value in at least one autoimmune disease, multiple sclerosis. The functionalized Hsp60-VLPs showed their significant increase in sensing potency when compared to monoclonal antibody detection of the peptide in a conventional immunoassay. Additionally, the developed Hsp60-VLPs allowed the detection of autoantibodies against the Hsp60 peptide in an in vivo mouse model of dextran sodium sulfate (DSS)-induced colitis. The detection of minute amounts of the autoantibodies allowed us to perform the analysis of their evolution during the progression of the disease. The anti-Hsp60 autoantibody levels in the sera of the inflamed mice went down during the induction phase of the disease. Increased levels of the anti-HSP60 autoantibodies were detected during the resolution phase of the disease. An extension of a previously proposed model for the involvement of Hsp60 in inflammatory processes is considered, incorporating a role for Hsp60 autoantibodies. This, and related models, can now be experimentally tested thanks to the autoantibody detection hypersensitivity provided by the functionalized VLPs.https://www.mdpi.com/2079-4991/9/10/1438vnpshsp60ibdautoantibodyinflammationdiagnosis |
| spellingShingle | Carmen Yuste-Calvo Mercedes López-Santalla Lucía Zurita César F. Cruz-Fernández Flora Sánchez Marina I. Garín Fernando Ponz Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection Nanomaterials vnps hsp60 ibd autoantibody inflammation diagnosis |
| title | Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection |
| title_full | Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection |
| title_fullStr | Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection |
| title_full_unstemmed | Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection |
| title_short | Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection |
| title_sort | elongated flexuous plant virus derived nanoparticles functionalized for autoantibody detection |
| topic | vnps hsp60 ibd autoantibody inflammation diagnosis |
| url | https://www.mdpi.com/2079-4991/9/10/1438 |
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