In vitro assessment of antimicrobial, anti-inflammatory, and schistolarvicidal activity of macroalgae-based gold nanoparticles
There is a growing need to improve facile, eco-friendly, and cheap approaches for nanoparticle (NP) synthesis. Green protocols have been investigated for the fabrication of NPs using several natural sources as plants, algae, fungi, and bacteria. Thus, the present study proposed a rapid, convenient,...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.1075832/full |
| _version_ | 1811185332192804864 |
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| author | Marwa Kamal Neveen Abdel-Raouf Neveen Abdel-Raouf Hana Sonbol Heba Abdel-Tawab Mohamed Sayed Abdelhameed Ola Hammouda Khaled N. M. Elsayed |
| author_facet | Marwa Kamal Neveen Abdel-Raouf Neveen Abdel-Raouf Hana Sonbol Heba Abdel-Tawab Mohamed Sayed Abdelhameed Ola Hammouda Khaled N. M. Elsayed |
| author_sort | Marwa Kamal |
| collection | DOAJ |
| description | There is a growing need to improve facile, eco-friendly, and cheap approaches for nanoparticle (NP) synthesis. Green protocols have been investigated for the fabrication of NPs using several natural sources as plants, algae, fungi, and bacteria. Thus, the present study proposed a rapid, convenient, and efficient biosynthesis of gold NPs (Au-NPs) using the ethanolic extracts of three macroalgae, i.e., Cystoseira myrica, C. trinodis, and Caulerpa prolifera. The reduction of Au ions and the fabrication of Au-NPs were validated using ultraviolet-visible (UV–Vis) spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), and zeta potential analysis. The produced Au-NPs were tested for their antibacterial, antifungal, anti-inflammatory, and schistolarvicidal activity. Results revealed the formation of Au-NPs with an average size of 12.6–15.5 nm and different shapes that are mainly spherical with pure crystalline nature. The strong antibacterial activities of C. trinodis– and C. myrica–based Au-NPs against E. coli (inhibition zones of 22 and 19 mm) and against Staphylococcus aureus (inhibition zones of 18 and 20.5 and mm) were recorded, respectively. On the other hand, the high antifungal activity of C. trinodis Au-NPs against Aspergillus niger and Alternaria alternate showed the inhibition zones of 18 and 17 mm, respectively. The high antifungal activity of C. trinodis Au-NPs against Candida albicans (inhibition zone 16 mm) was also recorded. Regarding anti-inflammatory and schistolarvicidal activity, Au-NPs fabricated using C. myrica showed 64.2% of the inhibitory effect on protein denaturation and recorded the highest schistolarvicidal activity against Schistosoma mansoni cercariae that sank and died after 7 min. Overall, these findings proved that macroalgal ethanolic extracts can be effectively used for the biosynthesis of Au-NPs. These Au-NPs offer a significant alternative antimicrobial, anti-inflammatory, and schistolarvicidal agents. for biomedical uses. |
| first_indexed | 2024-04-11T13:27:42Z |
| format | Article |
| id | doaj.art-548bdda206784caaa12c45ffe15d34ac |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-11T13:27:42Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-548bdda206784caaa12c45ffe15d34ac2022-12-22T04:21:58ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-12-01910.3389/fmars.2022.10758321075832In vitro assessment of antimicrobial, anti-inflammatory, and schistolarvicidal activity of macroalgae-based gold nanoparticlesMarwa Kamal0Neveen Abdel-Raouf1Neveen Abdel-Raouf2Hana Sonbol3Heba Abdel-Tawab4Mohamed Sayed Abdelhameed5Ola Hammouda6Khaled N. M. Elsayed7Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, EgyptBotany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, EgyptDepartment of Biology, College of Science and Humanities in Al-Kharj, Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi ArabiaDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi ArabiaZoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, EgyptBotany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, EgyptBotany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, EgyptBotany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, EgyptThere is a growing need to improve facile, eco-friendly, and cheap approaches for nanoparticle (NP) synthesis. Green protocols have been investigated for the fabrication of NPs using several natural sources as plants, algae, fungi, and bacteria. Thus, the present study proposed a rapid, convenient, and efficient biosynthesis of gold NPs (Au-NPs) using the ethanolic extracts of three macroalgae, i.e., Cystoseira myrica, C. trinodis, and Caulerpa prolifera. The reduction of Au ions and the fabrication of Au-NPs were validated using ultraviolet-visible (UV–Vis) spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), and zeta potential analysis. The produced Au-NPs were tested for their antibacterial, antifungal, anti-inflammatory, and schistolarvicidal activity. Results revealed the formation of Au-NPs with an average size of 12.6–15.5 nm and different shapes that are mainly spherical with pure crystalline nature. The strong antibacterial activities of C. trinodis– and C. myrica–based Au-NPs against E. coli (inhibition zones of 22 and 19 mm) and against Staphylococcus aureus (inhibition zones of 18 and 20.5 and mm) were recorded, respectively. On the other hand, the high antifungal activity of C. trinodis Au-NPs against Aspergillus niger and Alternaria alternate showed the inhibition zones of 18 and 17 mm, respectively. The high antifungal activity of C. trinodis Au-NPs against Candida albicans (inhibition zone 16 mm) was also recorded. Regarding anti-inflammatory and schistolarvicidal activity, Au-NPs fabricated using C. myrica showed 64.2% of the inhibitory effect on protein denaturation and recorded the highest schistolarvicidal activity against Schistosoma mansoni cercariae that sank and died after 7 min. Overall, these findings proved that macroalgal ethanolic extracts can be effectively used for the biosynthesis of Au-NPs. These Au-NPs offer a significant alternative antimicrobial, anti-inflammatory, and schistolarvicidal agents. for biomedical uses.https://www.frontiersin.org/articles/10.3389/fmars.2022.1075832/fullbiosynthesized gold nanoparticlesmacroalgaecystosiera myricacystosiera trinodiscaulerpa proliferaantimicrobial |
| spellingShingle | Marwa Kamal Neveen Abdel-Raouf Neveen Abdel-Raouf Hana Sonbol Heba Abdel-Tawab Mohamed Sayed Abdelhameed Ola Hammouda Khaled N. M. Elsayed In vitro assessment of antimicrobial, anti-inflammatory, and schistolarvicidal activity of macroalgae-based gold nanoparticles Frontiers in Marine Science biosynthesized gold nanoparticles macroalgae cystosiera myrica cystosiera trinodis caulerpa prolifera antimicrobial |
| title | In vitro assessment of antimicrobial, anti-inflammatory, and schistolarvicidal activity of macroalgae-based gold nanoparticles |
| title_full | In vitro assessment of antimicrobial, anti-inflammatory, and schistolarvicidal activity of macroalgae-based gold nanoparticles |
| title_fullStr | In vitro assessment of antimicrobial, anti-inflammatory, and schistolarvicidal activity of macroalgae-based gold nanoparticles |
| title_full_unstemmed | In vitro assessment of antimicrobial, anti-inflammatory, and schistolarvicidal activity of macroalgae-based gold nanoparticles |
| title_short | In vitro assessment of antimicrobial, anti-inflammatory, and schistolarvicidal activity of macroalgae-based gold nanoparticles |
| title_sort | in vitro assessment of antimicrobial anti inflammatory and schistolarvicidal activity of macroalgae based gold nanoparticles |
| topic | biosynthesized gold nanoparticles macroalgae cystosiera myrica cystosiera trinodis caulerpa prolifera antimicrobial |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.1075832/full |
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