Expanding the limits of the second genetic code with ribozymes
Flexizymes have been used to expand the scope of chemical substrates for ribosome-directed polymerization in vitro. Here the authors deduce design rules of Flexizyme-mediated tRNA acylation that more effectively predict the incorporation of new monomers into peptides.
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2019-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-12916-w |
| _version_ | 1818428817794924544 |
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| author | Joongoo Lee Kenneth E. Schwieter Andrew M. Watkins Do Soon Kim Hao Yu Kevin J. Schwarz Jongdoo Lim Jaime Coronado Michelle Byrom Eric V. Anslyn Andrew D. Ellington Jeffrey S. Moore Michael C. Jewett |
| author_facet | Joongoo Lee Kenneth E. Schwieter Andrew M. Watkins Do Soon Kim Hao Yu Kevin J. Schwarz Jongdoo Lim Jaime Coronado Michelle Byrom Eric V. Anslyn Andrew D. Ellington Jeffrey S. Moore Michael C. Jewett |
| author_sort | Joongoo Lee |
| collection | DOAJ |
| description | Flexizymes have been used to expand the scope of chemical substrates for ribosome-directed polymerization in vitro. Here the authors deduce design rules of Flexizyme-mediated tRNA acylation that more effectively predict the incorporation of new monomers into peptides. |
| first_indexed | 2024-12-14T15:07:39Z |
| format | Article |
| id | doaj.art-548c054981ad4d0a9ebff922fc32ffbd |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-14T15:07:39Z |
| publishDate | 2019-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-548c054981ad4d0a9ebff922fc32ffbd2022-12-21T22:56:40ZengNature PortfolioNature Communications2041-17232019-11-0110111210.1038/s41467-019-12916-wExpanding the limits of the second genetic code with ribozymesJoongoo Lee0Kenneth E. Schwieter1Andrew M. Watkins2Do Soon Kim3Hao Yu4Kevin J. Schwarz5Jongdoo Lim6Jaime Coronado7Michelle Byrom8Eric V. Anslyn9Andrew D. Ellington10Jeffrey S. Moore11Michael C. Jewett12Department of Chemical and Biological Engineering, Northwestern UniversityDepartment of Chemistry, University of Illinois at Urbana−ChampaignDepartments of Biochemistry and Physics, Stanford UniversityDepartment of Chemical and Biological Engineering, Northwestern UniversityDepartments of Chemical and Biomolecular Engineering, University of Illinois at Urbana−ChampaignDepartment of Chemistry, University of Illinois at Urbana−ChampaignDepartment of Chemistry, University of Texas at AustinDepartment of Chemistry, University of Texas at AustinDepartment of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at AustinDepartment of Chemistry, University of Texas at AustinDepartment of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at AustinDepartment of Chemistry, University of Illinois at Urbana−ChampaignDepartment of Chemical and Biological Engineering, Northwestern UniversityFlexizymes have been used to expand the scope of chemical substrates for ribosome-directed polymerization in vitro. Here the authors deduce design rules of Flexizyme-mediated tRNA acylation that more effectively predict the incorporation of new monomers into peptides.https://doi.org/10.1038/s41467-019-12916-w |
| spellingShingle | Joongoo Lee Kenneth E. Schwieter Andrew M. Watkins Do Soon Kim Hao Yu Kevin J. Schwarz Jongdoo Lim Jaime Coronado Michelle Byrom Eric V. Anslyn Andrew D. Ellington Jeffrey S. Moore Michael C. Jewett Expanding the limits of the second genetic code with ribozymes Nature Communications |
| title | Expanding the limits of the second genetic code with ribozymes |
| title_full | Expanding the limits of the second genetic code with ribozymes |
| title_fullStr | Expanding the limits of the second genetic code with ribozymes |
| title_full_unstemmed | Expanding the limits of the second genetic code with ribozymes |
| title_short | Expanding the limits of the second genetic code with ribozymes |
| title_sort | expanding the limits of the second genetic code with ribozymes |
| url | https://doi.org/10.1038/s41467-019-12916-w |
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