Reliable and Rapid Detection and Quantification of Enrofloxacin Using a Ratiometric SERS Aptasensor
Reliable detection and quantification of antibiotic residues in food using surface-enhanced Raman spectroscopy remain challenging, since the intensities of SERS signals are vulnerable to matrix and experimental factors. In this work, a ratiometric SERS aptasensor using 6-Carboxyl-X-Rhodamine (ROX)-l...
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MDPI AG
2022-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/24/8764 |
| _version_ | 1797456072533868544 |
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| author | Panxue Wang Li Wang Cen Li Xiang Li Guoliang Li |
| author_facet | Panxue Wang Li Wang Cen Li Xiang Li Guoliang Li |
| author_sort | Panxue Wang |
| collection | DOAJ |
| description | Reliable detection and quantification of antibiotic residues in food using surface-enhanced Raman spectroscopy remain challenging, since the intensities of SERS signals are vulnerable to matrix and experimental factors. In this work, a ratiometric SERS aptasensor using 6-Carboxyl-X-Rhodamine (ROX)-labeled aptamers and 4-mercaptobenzonitrile (4-MBN)-functionalized gold nanoparticles (Au NPs) as SERS probes was established for the reliable and rapid detection and quantification of enrofloxacin. In the presence of enrofloxacin, the conformational transform of aptamers took place, and the distance between ROX and Au NP increased, which resulted in a decrease in the SERS signal intensity of ROX. Meanwhile, the intensity of the SERS signal of 4-MBN was used as an internal standard. Reliable determination of enrofloxacin was realized using the ratio of the SERS signal intensities of ROX to 4-MBN. Under optimal conditions, the developed ratiometric SERS aptasensor provided a wide linear range from 5 nM to 1 µM, with a correlation coefficient (R<sup>2</sup>) of 0.98 and a limit of detection (LOD) of 0.12 nM (0.043 ppb). In addition, the developed ratiometric SERS aptasensor was successfully applied for the determination of enrofloxacin in fish and chicken meat, with recovery values of 93.6–112.0%. Therefore, the established ratiometric SERS aptasensor is sensitive, reliable, time-efficient, and has the potential to be applied in the on-site detection of enrofloxacin in complex matrices. |
| first_indexed | 2024-03-09T16:03:09Z |
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| id | doaj.art-548f63d7f8c9461f9eccc204dbe385fa |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T16:03:09Z |
| publishDate | 2022-12-01 |
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| series | Molecules |
| spelling | doaj.art-548f63d7f8c9461f9eccc204dbe385fa2023-11-24T16:57:00ZengMDPI AGMolecules1420-30492022-12-012724876410.3390/molecules27248764Reliable and Rapid Detection and Quantification of Enrofloxacin Using a Ratiometric SERS AptasensorPanxue Wang0Li Wang1Cen Li2Xiang Li3Guoliang Li4School of Food Science and Engineering, Shaanxi University of Science & Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science & Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science & Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science & Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science & Technology, Xi’an 710021, ChinaReliable detection and quantification of antibiotic residues in food using surface-enhanced Raman spectroscopy remain challenging, since the intensities of SERS signals are vulnerable to matrix and experimental factors. In this work, a ratiometric SERS aptasensor using 6-Carboxyl-X-Rhodamine (ROX)-labeled aptamers and 4-mercaptobenzonitrile (4-MBN)-functionalized gold nanoparticles (Au NPs) as SERS probes was established for the reliable and rapid detection and quantification of enrofloxacin. In the presence of enrofloxacin, the conformational transform of aptamers took place, and the distance between ROX and Au NP increased, which resulted in a decrease in the SERS signal intensity of ROX. Meanwhile, the intensity of the SERS signal of 4-MBN was used as an internal standard. Reliable determination of enrofloxacin was realized using the ratio of the SERS signal intensities of ROX to 4-MBN. Under optimal conditions, the developed ratiometric SERS aptasensor provided a wide linear range from 5 nM to 1 µM, with a correlation coefficient (R<sup>2</sup>) of 0.98 and a limit of detection (LOD) of 0.12 nM (0.043 ppb). In addition, the developed ratiometric SERS aptasensor was successfully applied for the determination of enrofloxacin in fish and chicken meat, with recovery values of 93.6–112.0%. Therefore, the established ratiometric SERS aptasensor is sensitive, reliable, time-efficient, and has the potential to be applied in the on-site detection of enrofloxacin in complex matrices.https://www.mdpi.com/1420-3049/27/24/8764enrofloxacindetectionSERS aptasensorratiometricportable Raman spectrometerfood safety |
| spellingShingle | Panxue Wang Li Wang Cen Li Xiang Li Guoliang Li Reliable and Rapid Detection and Quantification of Enrofloxacin Using a Ratiometric SERS Aptasensor Molecules enrofloxacin detection SERS aptasensor ratiometric portable Raman spectrometer food safety |
| title | Reliable and Rapid Detection and Quantification of Enrofloxacin Using a Ratiometric SERS Aptasensor |
| title_full | Reliable and Rapid Detection and Quantification of Enrofloxacin Using a Ratiometric SERS Aptasensor |
| title_fullStr | Reliable and Rapid Detection and Quantification of Enrofloxacin Using a Ratiometric SERS Aptasensor |
| title_full_unstemmed | Reliable and Rapid Detection and Quantification of Enrofloxacin Using a Ratiometric SERS Aptasensor |
| title_short | Reliable and Rapid Detection and Quantification of Enrofloxacin Using a Ratiometric SERS Aptasensor |
| title_sort | reliable and rapid detection and quantification of enrofloxacin using a ratiometric sers aptasensor |
| topic | enrofloxacin detection SERS aptasensor ratiometric portable Raman spectrometer food safety |
| url | https://www.mdpi.com/1420-3049/27/24/8764 |
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