Modified Crosstalk between Phytohormones in Arabidopsis Mutants for PEP-Associated Proteins
Plastid-encoded RNA polymerase (PEP) forms a multisubunit complex in operating chloroplasts, where PEP subunits and a sigma factor are tightly associated with 12 additional nuclear-encoded proteins. Mutants with disrupted genes encoding PEP-associated proteins (PAPs) provide unique tools for deciphe...
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MDPI AG
2024-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/3/1586 |
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| author | Ivan A. Bychkov Aleksandra A. Andreeva Radomira Vankova Jozef Lacek Natalia V. Kudryakova Victor V. Kusnetsov |
| author_facet | Ivan A. Bychkov Aleksandra A. Andreeva Radomira Vankova Jozef Lacek Natalia V. Kudryakova Victor V. Kusnetsov |
| author_sort | Ivan A. Bychkov |
| collection | DOAJ |
| description | Plastid-encoded RNA polymerase (PEP) forms a multisubunit complex in operating chloroplasts, where PEP subunits and a sigma factor are tightly associated with 12 additional nuclear-encoded proteins. Mutants with disrupted genes encoding PEP-associated proteins (PAPs) provide unique tools for deciphering mutual relationships among phytohormones. A block of chloroplast biogenesis in <i>Arabidopsis pap</i> mutants specifying highly altered metabolism in white tissues induced dramatic fluctuations in the content of major phytohormones and their metabolic genes, whereas hormone signaling circuits mostly remained functional. Reprogramming of the expression of biosynthetic and metabolic genes contributed to a greatly increased content of salicylic acid (SA) and a concomitant decrease in 1-aminocyclopropane-1-carboxylic acid (ACC) and oxophytodienoic acid (OPDA), precursors of ethylene and jasmonic acid, respectively, in parallel to reduced levels of abscisic acid (ABA). The lack of differences in the free levels of indole-3-acetic acid (IAA) between the <i>pap</i> mutants and wild-type plants was accompanied by fluctuations in the contents of IAA precursors and conjugated forms as well as multilayered changes in the expression of IAA metabolic genes. Along with cytokinin (CK) overproduction, all of these compensatory changes aim to balance plant growth and defense systems to ensure viability under highly modulated conditions. |
| first_indexed | 2024-03-08T03:55:32Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T03:55:32Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-549392f591da4411b2b2bd57cf22f2922024-02-09T15:13:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-01-01253158610.3390/ijms25031586Modified Crosstalk between Phytohormones in Arabidopsis Mutants for PEP-Associated ProteinsIvan A. Bychkov0Aleksandra A. Andreeva1Radomira Vankova2Jozef Lacek3Natalia V. Kudryakova4Victor V. Kusnetsov5Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, Moscow 127276, RussiaTimiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, Moscow 127276, RussiaInstitute of Experimental Botany, Academy of Sciences CR, Rozvojova 263, 165 02 Prague, Czech RepublicInstitute of Experimental Botany, Academy of Sciences CR, Rozvojova 263, 165 02 Prague, Czech RepublicTimiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, Moscow 127276, RussiaTimiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, Moscow 127276, RussiaPlastid-encoded RNA polymerase (PEP) forms a multisubunit complex in operating chloroplasts, where PEP subunits and a sigma factor are tightly associated with 12 additional nuclear-encoded proteins. Mutants with disrupted genes encoding PEP-associated proteins (PAPs) provide unique tools for deciphering mutual relationships among phytohormones. A block of chloroplast biogenesis in <i>Arabidopsis pap</i> mutants specifying highly altered metabolism in white tissues induced dramatic fluctuations in the content of major phytohormones and their metabolic genes, whereas hormone signaling circuits mostly remained functional. Reprogramming of the expression of biosynthetic and metabolic genes contributed to a greatly increased content of salicylic acid (SA) and a concomitant decrease in 1-aminocyclopropane-1-carboxylic acid (ACC) and oxophytodienoic acid (OPDA), precursors of ethylene and jasmonic acid, respectively, in parallel to reduced levels of abscisic acid (ABA). The lack of differences in the free levels of indole-3-acetic acid (IAA) between the <i>pap</i> mutants and wild-type plants was accompanied by fluctuations in the contents of IAA precursors and conjugated forms as well as multilayered changes in the expression of IAA metabolic genes. Along with cytokinin (CK) overproduction, all of these compensatory changes aim to balance plant growth and defense systems to ensure viability under highly modulated conditions.https://www.mdpi.com/1422-0067/25/3/1586<i>Arabidopsis thaliana</i>phytohormonesgene expressionPEP-associated proteinsmutants |
| spellingShingle | Ivan A. Bychkov Aleksandra A. Andreeva Radomira Vankova Jozef Lacek Natalia V. Kudryakova Victor V. Kusnetsov Modified Crosstalk between Phytohormones in Arabidopsis Mutants for PEP-Associated Proteins International Journal of Molecular Sciences <i>Arabidopsis thaliana</i> phytohormones gene expression PEP-associated proteins mutants |
| title | Modified Crosstalk between Phytohormones in Arabidopsis Mutants for PEP-Associated Proteins |
| title_full | Modified Crosstalk between Phytohormones in Arabidopsis Mutants for PEP-Associated Proteins |
| title_fullStr | Modified Crosstalk between Phytohormones in Arabidopsis Mutants for PEP-Associated Proteins |
| title_full_unstemmed | Modified Crosstalk between Phytohormones in Arabidopsis Mutants for PEP-Associated Proteins |
| title_short | Modified Crosstalk between Phytohormones in Arabidopsis Mutants for PEP-Associated Proteins |
| title_sort | modified crosstalk between phytohormones in arabidopsis mutants for pep associated proteins |
| topic | <i>Arabidopsis thaliana</i> phytohormones gene expression PEP-associated proteins mutants |
| url | https://www.mdpi.com/1422-0067/25/3/1586 |
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