The genetic diversity and relationships of cauliflower (Brassica oleracea var. botrytis) inbred lines assessed by using SSR markers.
Inbred lines are important germplasm in cauliflower breeding programs. To understand the genetic diversity and relationships of cauliflower inbred lines, the use of simple sequence repeat (SSR) markers will be of great value for parental line selection and breeding strategy design. In this study, th...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0208551 |
| _version_ | 1818403725765509120 |
|---|---|
| author | Shiyang Zhu Xiaoling Zhang Qing Liu Tiankuan Luo Zheng Tang Yuanchang Zhou |
| author_facet | Shiyang Zhu Xiaoling Zhang Qing Liu Tiankuan Luo Zheng Tang Yuanchang Zhou |
| author_sort | Shiyang Zhu |
| collection | DOAJ |
| description | Inbred lines are important germplasm in cauliflower breeding programs. To understand the genetic diversity and relationships of cauliflower inbred lines, the use of simple sequence repeat (SSR) markers will be of great value for parental line selection and breeding strategy design. In this study, the genetic diversity and relationships of 165 cauliflower inbred lines primarily derived from southeast China were assessed using SSR markers. Forty-three SSR markers were polymorphic across these inbred lines and generated a total of 111 alleles. The mean values of the number of alleles (Na), effective number of alleles (Ne), Shannon's Information index (I), and polymorphism information content (PIC) per locus were 2.581, 1.599, 0.517 and 0.316, respectively. Genetic distance values among all pairs of the inbred lines varied from 0 to 0.67 with an average of 0.30. On the basis of genetic distance data estimated with the SSR markers, the 165 cauliflower inbred lines were classified into four main clusters (from group Ⅰ to group Ⅳ) by cluster analysis and four subpopulations (from POP 1 to POP 4) by structure analysis. The classification patterns of most cauliflower inbred lines were not consistent with their curd maturity, curd solidity or geographic origins. These results based on estimates by the SSR markers, suggested the genetic diversity of the 165 cauliflower inbred lines was relatively narrow. Therefore, pyramiding the valuable genes among different types of the cauliflower inbred lines is important to increase the genetic diversity to obtain desirable hybridization combinations. The information generated in this report will be useful for assessing germplasm and breeding in cauliflower. |
| first_indexed | 2024-12-14T08:28:50Z |
| format | Article |
| id | doaj.art-5494376ccb534660932807b0e6ed0cbe |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T08:28:50Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5494376ccb534660932807b0e6ed0cbe2022-12-21T23:09:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-011312e020855110.1371/journal.pone.0208551The genetic diversity and relationships of cauliflower (Brassica oleracea var. botrytis) inbred lines assessed by using SSR markers.Shiyang ZhuXiaoling ZhangQing LiuTiankuan LuoZheng TangYuanchang ZhouInbred lines are important germplasm in cauliflower breeding programs. To understand the genetic diversity and relationships of cauliflower inbred lines, the use of simple sequence repeat (SSR) markers will be of great value for parental line selection and breeding strategy design. In this study, the genetic diversity and relationships of 165 cauliflower inbred lines primarily derived from southeast China were assessed using SSR markers. Forty-three SSR markers were polymorphic across these inbred lines and generated a total of 111 alleles. The mean values of the number of alleles (Na), effective number of alleles (Ne), Shannon's Information index (I), and polymorphism information content (PIC) per locus were 2.581, 1.599, 0.517 and 0.316, respectively. Genetic distance values among all pairs of the inbred lines varied from 0 to 0.67 with an average of 0.30. On the basis of genetic distance data estimated with the SSR markers, the 165 cauliflower inbred lines were classified into four main clusters (from group Ⅰ to group Ⅳ) by cluster analysis and four subpopulations (from POP 1 to POP 4) by structure analysis. The classification patterns of most cauliflower inbred lines were not consistent with their curd maturity, curd solidity or geographic origins. These results based on estimates by the SSR markers, suggested the genetic diversity of the 165 cauliflower inbred lines was relatively narrow. Therefore, pyramiding the valuable genes among different types of the cauliflower inbred lines is important to increase the genetic diversity to obtain desirable hybridization combinations. The information generated in this report will be useful for assessing germplasm and breeding in cauliflower.https://doi.org/10.1371/journal.pone.0208551 |
| spellingShingle | Shiyang Zhu Xiaoling Zhang Qing Liu Tiankuan Luo Zheng Tang Yuanchang Zhou The genetic diversity and relationships of cauliflower (Brassica oleracea var. botrytis) inbred lines assessed by using SSR markers. PLoS ONE |
| title | The genetic diversity and relationships of cauliflower (Brassica oleracea var. botrytis) inbred lines assessed by using SSR markers. |
| title_full | The genetic diversity and relationships of cauliflower (Brassica oleracea var. botrytis) inbred lines assessed by using SSR markers. |
| title_fullStr | The genetic diversity and relationships of cauliflower (Brassica oleracea var. botrytis) inbred lines assessed by using SSR markers. |
| title_full_unstemmed | The genetic diversity and relationships of cauliflower (Brassica oleracea var. botrytis) inbred lines assessed by using SSR markers. |
| title_short | The genetic diversity and relationships of cauliflower (Brassica oleracea var. botrytis) inbred lines assessed by using SSR markers. |
| title_sort | genetic diversity and relationships of cauliflower brassica oleracea var botrytis inbred lines assessed by using ssr markers |
| url | https://doi.org/10.1371/journal.pone.0208551 |
| work_keys_str_mv | AT shiyangzhu thegeneticdiversityandrelationshipsofcauliflowerbrassicaoleraceavarbotrytisinbredlinesassessedbyusingssrmarkers AT xiaolingzhang thegeneticdiversityandrelationshipsofcauliflowerbrassicaoleraceavarbotrytisinbredlinesassessedbyusingssrmarkers AT qingliu thegeneticdiversityandrelationshipsofcauliflowerbrassicaoleraceavarbotrytisinbredlinesassessedbyusingssrmarkers AT tiankuanluo thegeneticdiversityandrelationshipsofcauliflowerbrassicaoleraceavarbotrytisinbredlinesassessedbyusingssrmarkers AT zhengtang thegeneticdiversityandrelationshipsofcauliflowerbrassicaoleraceavarbotrytisinbredlinesassessedbyusingssrmarkers AT yuanchangzhou thegeneticdiversityandrelationshipsofcauliflowerbrassicaoleraceavarbotrytisinbredlinesassessedbyusingssrmarkers AT shiyangzhu geneticdiversityandrelationshipsofcauliflowerbrassicaoleraceavarbotrytisinbredlinesassessedbyusingssrmarkers AT xiaolingzhang geneticdiversityandrelationshipsofcauliflowerbrassicaoleraceavarbotrytisinbredlinesassessedbyusingssrmarkers AT qingliu geneticdiversityandrelationshipsofcauliflowerbrassicaoleraceavarbotrytisinbredlinesassessedbyusingssrmarkers AT tiankuanluo geneticdiversityandrelationshipsofcauliflowerbrassicaoleraceavarbotrytisinbredlinesassessedbyusingssrmarkers AT zhengtang geneticdiversityandrelationshipsofcauliflowerbrassicaoleraceavarbotrytisinbredlinesassessedbyusingssrmarkers AT yuanchangzhou geneticdiversityandrelationshipsofcauliflowerbrassicaoleraceavarbotrytisinbredlinesassessedbyusingssrmarkers |