Development and evaluation of a prototype non-woven fabric filter for purification of malaria-infected blood
<p>Abstract</p> <p>Background</p> <p>Many malaria-related studies depend on infected red blood cells (iRBCs) as fundamental material; however, infected blood samples from human or animal models include leukocytes (white blood cells or WBCs), especially difficult to sepa...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-08-01
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| Series: | Malaria Journal |
| Online Access: | http://www.malariajournal.com/content/10/1/251 |
| _version_ | 1818790989263798272 |
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| author | Tao Zhi-Yong Xia Hui Cao Jun Gao Qi |
| author_facet | Tao Zhi-Yong Xia Hui Cao Jun Gao Qi |
| author_sort | Tao Zhi-Yong |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Many malaria-related studies depend on infected red blood cells (iRBCs) as fundamental material; however, infected blood samples from human or animal models include leukocytes (white blood cells or WBCs), especially difficult to separate from iRBCs in cases involving <it>Plasmodium vivax</it>. These host WBCs are a source of contamination in biology, immunology and molecular biology studies, requiring their removal. Non-woven fabric (NWF) has the ability to adsorb leukocytes and is already used as filtration material to deplete WBCs for blood transfusion and surgery. The present study describes the development and evaluation of a prototype NWF filter designed for purifying iRBCs from malaria-infected blood.</p> <p>Methods</p> <p>Blood samples of <it>P. vivax </it>patients were processed separately by NWF filter and CF11 column methods. WBCs and RBCs were counted, parasite density, morphology and developing stage was checked by microscopy, and compared before and after treatment. The viability of filtrated <it>P. vivax </it>parasites was examined by <it>in vitro </it>short-term cultivation.</p> <p>Results</p> <p>A total of 15 <it>P. vivax</it>-infected blood samples were treated by both NWF filter and CF11 methods. The WBC removal rate of the NWF filter method was 99.03%, significantly higher than the CF11 methods (98.41%, <it>P </it>< 0.01). The RBC recovery rate of the NWF filter method was 95.48%, also significantly higher than the CF11 method (87.05%, <it>P </it>< 0.01). Fourteen <it>in vitro </it>short-term culture results showed that after filter treatment, <it>P. vivax </it>parasite could develop as normal as CF11 method, and no obvious density, developing stage difference were fund between two methods.</p> <p>Conclusions</p> <p>NWF filter filtration removed most leukocytes from malaria-infected blood, and the recovery rate of RBCs was higher than with CF11 column method. Filtrated <it>P. vivax </it>parasites were morphologically normal, viable, and suitable for short-term <it>in vitro </it>culture. NWF filter filtration is simple, fast and robust, and is ideal for purification of malaria-infected blood.</p> |
| first_indexed | 2024-12-18T15:04:13Z |
| format | Article |
| id | doaj.art-5496d51d6a1c4b54a5cbdf15fd6c4ff8 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-18T15:04:13Z |
| publishDate | 2011-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-5496d51d6a1c4b54a5cbdf15fd6c4ff82022-12-21T21:03:48ZengBMCMalaria Journal1475-28752011-08-0110125110.1186/1475-2875-10-251Development and evaluation of a prototype non-woven fabric filter for purification of malaria-infected bloodTao Zhi-YongXia HuiCao JunGao Qi<p>Abstract</p> <p>Background</p> <p>Many malaria-related studies depend on infected red blood cells (iRBCs) as fundamental material; however, infected blood samples from human or animal models include leukocytes (white blood cells or WBCs), especially difficult to separate from iRBCs in cases involving <it>Plasmodium vivax</it>. These host WBCs are a source of contamination in biology, immunology and molecular biology studies, requiring their removal. Non-woven fabric (NWF) has the ability to adsorb leukocytes and is already used as filtration material to deplete WBCs for blood transfusion and surgery. The present study describes the development and evaluation of a prototype NWF filter designed for purifying iRBCs from malaria-infected blood.</p> <p>Methods</p> <p>Blood samples of <it>P. vivax </it>patients were processed separately by NWF filter and CF11 column methods. WBCs and RBCs were counted, parasite density, morphology and developing stage was checked by microscopy, and compared before and after treatment. The viability of filtrated <it>P. vivax </it>parasites was examined by <it>in vitro </it>short-term cultivation.</p> <p>Results</p> <p>A total of 15 <it>P. vivax</it>-infected blood samples were treated by both NWF filter and CF11 methods. The WBC removal rate of the NWF filter method was 99.03%, significantly higher than the CF11 methods (98.41%, <it>P </it>< 0.01). The RBC recovery rate of the NWF filter method was 95.48%, also significantly higher than the CF11 method (87.05%, <it>P </it>< 0.01). Fourteen <it>in vitro </it>short-term culture results showed that after filter treatment, <it>P. vivax </it>parasite could develop as normal as CF11 method, and no obvious density, developing stage difference were fund between two methods.</p> <p>Conclusions</p> <p>NWF filter filtration removed most leukocytes from malaria-infected blood, and the recovery rate of RBCs was higher than with CF11 column method. Filtrated <it>P. vivax </it>parasites were morphologically normal, viable, and suitable for short-term <it>in vitro </it>culture. NWF filter filtration is simple, fast and robust, and is ideal for purification of malaria-infected blood.</p>http://www.malariajournal.com/content/10/1/251 |
| spellingShingle | Tao Zhi-Yong Xia Hui Cao Jun Gao Qi Development and evaluation of a prototype non-woven fabric filter for purification of malaria-infected blood Malaria Journal |
| title | Development and evaluation of a prototype non-woven fabric filter for purification of malaria-infected blood |
| title_full | Development and evaluation of a prototype non-woven fabric filter for purification of malaria-infected blood |
| title_fullStr | Development and evaluation of a prototype non-woven fabric filter for purification of malaria-infected blood |
| title_full_unstemmed | Development and evaluation of a prototype non-woven fabric filter for purification of malaria-infected blood |
| title_short | Development and evaluation of a prototype non-woven fabric filter for purification of malaria-infected blood |
| title_sort | development and evaluation of a prototype non woven fabric filter for purification of malaria infected blood |
| url | http://www.malariajournal.com/content/10/1/251 |
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