Development and Evaluation of Polyvinylpyrrolidone K90 and Poloxamer 407 Self-Assembled Nanomicelles: Enhanced Topical Ocular Delivery of Artemisinin
Age-related macular degeneration is a multifactorial disease affecting the posterior segment of the eye and is characterized by aberrant nascent blood vessels that leak blood and fluid. It ends with vision loss. In the present study, artemisinin which is poorly water-soluble and has potent anti-angi...
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MDPI AG
2021-09-01
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| Series: | Polymers |
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| author | Chandrasekar Ponnusamy Abimanyu Sugumaran Venkateshwaran Krishnaswami Rajaguru Palanichamy Ravichandiran Velayutham Subramanian Natesan |
| author_facet | Chandrasekar Ponnusamy Abimanyu Sugumaran Venkateshwaran Krishnaswami Rajaguru Palanichamy Ravichandiran Velayutham Subramanian Natesan |
| author_sort | Chandrasekar Ponnusamy |
| collection | DOAJ |
| description | Age-related macular degeneration is a multifactorial disease affecting the posterior segment of the eye and is characterized by aberrant nascent blood vessels that leak blood and fluid. It ends with vision loss. In the present study, artemisinin which is poorly water-soluble and has potent anti-angiogenic and anti-inflammatory properties was formulated into nanomicelles and characterized for its ocular application and anti-angiogenic activity using a CAM assay. Artemisinin-loaded nanomicelles were prepared by varying the concentrations of PVP k90 and poloxamer 407 at different ratios and showed spherical shape particles in the size range of 41–51 nm. The transparency and cloud point of the developed artemisinin-loaded nanomicelles was found to be 99–94% and 68–70 °C, respectively. The in vitro release of artemisinin from the nanomicelles was found to be 96.0–99.0% within 8 h. The trans-corneal permeation studies exhibited a 1.717–2.169 µg permeation of the artemisinin from nanomicelles through the excised rabbit eye cornea for 2 h. Drug-free nanomicelles did not exhibit noticeable DNA damage and showed an acceptable level of hemolytic potential. Artemisinin-loaded nanomicelles exhibited remarkable anti-angiogenic activity compared to artemisinin suspension. Hence, the formulated artemisinin-loaded nanomicelles might have the potential for the treatment of AMD. |
| first_indexed | 2024-03-10T07:17:20Z |
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| id | doaj.art-54976c2317af48e0b95eb59b027edee1 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T07:17:20Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-54976c2317af48e0b95eb59b027edee12023-11-22T14:55:06ZengMDPI AGPolymers2073-43602021-09-011318303810.3390/polym13183038Development and Evaluation of Polyvinylpyrrolidone K90 and Poloxamer 407 Self-Assembled Nanomicelles: Enhanced Topical Ocular Delivery of ArtemisininChandrasekar Ponnusamy0Abimanyu Sugumaran1Venkateshwaran Krishnaswami2Rajaguru Palanichamy3Ravichandiran Velayutham4Subramanian Natesan5Department of Pharmaceutical Technology, University College of Engineering, Bharathidasan Institute of Technology Campus, Anna University, Tiruchirappalli 620024, Tamil Nadu, IndiaDepartment of Pharmaceutics, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur 603203, Tamil Nadu, IndiaDepartment of Pharmaceutical Technology, University College of Engineering, Bharathidasan Institute of Technology Campus, Anna University, Tiruchirappalli 620024, Tamil Nadu, IndiaDepartment of Life Sciences, School of Life Sciences, Central University of Tamil Nadu, Tiruvarur 627007, Tamil Nadu, IndiaDepartment of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER)—Kolkata, Chunilal Bhawan, 168, Maniktala Main Road, Kolkata 700054, West Bengal, IndiaDepartment of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER)—Kolkata, Chunilal Bhawan, 168, Maniktala Main Road, Kolkata 700054, West Bengal, IndiaAge-related macular degeneration is a multifactorial disease affecting the posterior segment of the eye and is characterized by aberrant nascent blood vessels that leak blood and fluid. It ends with vision loss. In the present study, artemisinin which is poorly water-soluble and has potent anti-angiogenic and anti-inflammatory properties was formulated into nanomicelles and characterized for its ocular application and anti-angiogenic activity using a CAM assay. Artemisinin-loaded nanomicelles were prepared by varying the concentrations of PVP k90 and poloxamer 407 at different ratios and showed spherical shape particles in the size range of 41–51 nm. The transparency and cloud point of the developed artemisinin-loaded nanomicelles was found to be 99–94% and 68–70 °C, respectively. The in vitro release of artemisinin from the nanomicelles was found to be 96.0–99.0% within 8 h. The trans-corneal permeation studies exhibited a 1.717–2.169 µg permeation of the artemisinin from nanomicelles through the excised rabbit eye cornea for 2 h. Drug-free nanomicelles did not exhibit noticeable DNA damage and showed an acceptable level of hemolytic potential. Artemisinin-loaded nanomicelles exhibited remarkable anti-angiogenic activity compared to artemisinin suspension. Hence, the formulated artemisinin-loaded nanomicelles might have the potential for the treatment of AMD.https://www.mdpi.com/2073-4360/13/18/3038nanomicellesartemisinincorneatoxicity |
| spellingShingle | Chandrasekar Ponnusamy Abimanyu Sugumaran Venkateshwaran Krishnaswami Rajaguru Palanichamy Ravichandiran Velayutham Subramanian Natesan Development and Evaluation of Polyvinylpyrrolidone K90 and Poloxamer 407 Self-Assembled Nanomicelles: Enhanced Topical Ocular Delivery of Artemisinin Polymers nanomicelles artemisinin cornea toxicity |
| title | Development and Evaluation of Polyvinylpyrrolidone K90 and Poloxamer 407 Self-Assembled Nanomicelles: Enhanced Topical Ocular Delivery of Artemisinin |
| title_full | Development and Evaluation of Polyvinylpyrrolidone K90 and Poloxamer 407 Self-Assembled Nanomicelles: Enhanced Topical Ocular Delivery of Artemisinin |
| title_fullStr | Development and Evaluation of Polyvinylpyrrolidone K90 and Poloxamer 407 Self-Assembled Nanomicelles: Enhanced Topical Ocular Delivery of Artemisinin |
| title_full_unstemmed | Development and Evaluation of Polyvinylpyrrolidone K90 and Poloxamer 407 Self-Assembled Nanomicelles: Enhanced Topical Ocular Delivery of Artemisinin |
| title_short | Development and Evaluation of Polyvinylpyrrolidone K90 and Poloxamer 407 Self-Assembled Nanomicelles: Enhanced Topical Ocular Delivery of Artemisinin |
| title_sort | development and evaluation of polyvinylpyrrolidone k90 and poloxamer 407 self assembled nanomicelles enhanced topical ocular delivery of artemisinin |
| topic | nanomicelles artemisinin cornea toxicity |
| url | https://www.mdpi.com/2073-4360/13/18/3038 |
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