Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia
Abstract The global energy crisis and limited supply of petroleum fuels have rekindled the interest in utilizing a sustainable biomass to produce biofuel. Butanol, an advanced biofuel, is a superior renewable resource as it has a high energy content and is less hygroscopic than other candidates. At...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-020-01674-3 |
| _version_ | 1811232474258210816 |
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| author | Shubo Li Li Huang Chengzhu Ke Zongwen Pang Liming Liu |
| author_facet | Shubo Li Li Huang Chengzhu Ke Zongwen Pang Liming Liu |
| author_sort | Shubo Li |
| collection | DOAJ |
| description | Abstract The global energy crisis and limited supply of petroleum fuels have rekindled the interest in utilizing a sustainable biomass to produce biofuel. Butanol, an advanced biofuel, is a superior renewable resource as it has a high energy content and is less hygroscopic than other candidates. At present, the biobutanol route, employing acetone–butanol–ethanol (ABE) fermentation in Clostridium species, is not economically competitive due to the high cost of feedstocks, low butanol titer, and product inhibition. Based on an analysis of the physiological characteristics of solventogenic clostridia, current advances that enhance ABE fermentation from strain improvement to product separation were systematically reviewed, focusing on: (1) elucidating the metabolic pathway and regulation mechanism of butanol synthesis; (2) enhancing cellular performance and robustness through metabolic engineering, and (3) optimizing the process of ABE fermentation. Finally, perspectives on engineering and exploiting clostridia as cell factories to efficiently produce various chemicals and materials are also discussed. |
| first_indexed | 2024-04-12T11:03:23Z |
| format | Article |
| id | doaj.art-549832d0d8b444df84a7c483eecd4db8 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-12T11:03:23Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-549832d0d8b444df84a7c483eecd4db82022-12-22T03:35:51ZengBMCBiotechnology for Biofuels1754-68342020-03-0113112510.1186/s13068-020-01674-3Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridiaShubo Li0Li Huang1Chengzhu Ke2Zongwen Pang3Liming Liu4College of Light Industry and Food Engineering, Guangxi UniversityCollege of Light Industry and Food Engineering, Guangxi UniversityCollege of Light Industry and Food Engineering, Guangxi UniversityCollege of Life Science and Technology, Guangxi UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityAbstract The global energy crisis and limited supply of petroleum fuels have rekindled the interest in utilizing a sustainable biomass to produce biofuel. Butanol, an advanced biofuel, is a superior renewable resource as it has a high energy content and is less hygroscopic than other candidates. At present, the biobutanol route, employing acetone–butanol–ethanol (ABE) fermentation in Clostridium species, is not economically competitive due to the high cost of feedstocks, low butanol titer, and product inhibition. Based on an analysis of the physiological characteristics of solventogenic clostridia, current advances that enhance ABE fermentation from strain improvement to product separation were systematically reviewed, focusing on: (1) elucidating the metabolic pathway and regulation mechanism of butanol synthesis; (2) enhancing cellular performance and robustness through metabolic engineering, and (3) optimizing the process of ABE fermentation. Finally, perspectives on engineering and exploiting clostridia as cell factories to efficiently produce various chemicals and materials are also discussed.http://link.springer.com/article/10.1186/s13068-020-01674-3Acetone–butanol–ethanol fermentationSolventogenic clostridiaMetabolic characteristicsMetabolic engineeringStrain improvementProcess optimization |
| spellingShingle | Shubo Li Li Huang Chengzhu Ke Zongwen Pang Liming Liu Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia Biotechnology for Biofuels Acetone–butanol–ethanol fermentation Solventogenic clostridia Metabolic characteristics Metabolic engineering Strain improvement Process optimization |
| title | Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia |
| title_full | Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia |
| title_fullStr | Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia |
| title_full_unstemmed | Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia |
| title_short | Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia |
| title_sort | pathway dissection regulation engineering and application lessons learned from biobutanol production by solventogenic clostridia |
| topic | Acetone–butanol–ethanol fermentation Solventogenic clostridia Metabolic characteristics Metabolic engineering Strain improvement Process optimization |
| url | http://link.springer.com/article/10.1186/s13068-020-01674-3 |
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