Reward‐enhancing effect of methylphenidate is abolished in dopamine transporter knockout mice: A model of attention‐deficit/hyperactivity disorder
Abstract Aim Attention‐deficit/hyperactivity disorder is a heterogeneous neurobiological disorder that is characterized by inattention, impulsivity, and an increase in motor activity. Although methylphenidate has been used as a medication for decades, unknown is whether methylphenidate treatment can...
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Wiley
2018-09-01
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Series: | Neuropsychopharmacology Reports |
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Online Access: | https://doi.org/10.1002/npr2.12020 |
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author | Soichiro Ide Yuiko Ikekubo Jennifer Hua Yukio Takamatsu George R. Uhl Ichiro Sora Kazutaka Ikeda |
author_facet | Soichiro Ide Yuiko Ikekubo Jennifer Hua Yukio Takamatsu George R. Uhl Ichiro Sora Kazutaka Ikeda |
author_sort | Soichiro Ide |
collection | DOAJ |
description | Abstract Aim Attention‐deficit/hyperactivity disorder is a heterogeneous neurobiological disorder that is characterized by inattention, impulsivity, and an increase in motor activity. Although methylphenidate has been used as a medication for decades, unknown is whether methylphenidate treatment can cause drug dependence in patients with attention‐deficit/hyperactivity disorder. This study investigated the reward‐enhancing effects of methylphenidate using intracranial self‐stimulation in an animal model of attention‐deficit/hyperactivity disorder, dopamine transporter knockout mice. Methods For the intracranial self‐stimulation procedures, the mice were trained to nosepoke to receive direct electrical stimulation via an electrode that was implanted in the lateral hypothalamus. After the acquisition of nosepoke responding for intracranial self‐stimulation, the effects of methylphenidate on intracranial self‐stimulation were investigated. Results In the progressive‐ratio procedure, dopamine transporter knockout mice exhibited an increase in intracranial self‐stimulation compared with wild‐type mice. Treatment with 5 and 10 mg/kg methylphenidate increased intracranial self‐stimulation responding in wild‐type mice. Methylphenidate at the same doses did not affect intracranial self‐stimulation responding in dopamine transporter knockout mice. We then investigated the effects of high‐dose methylphenidate (60 mg/kg) in a rate‐frequency procedure. High‐dose methylphenidate significantly decreased intracranial self‐stimulation responding in both wild‐type and dopamine transporter knockout mice. Conclusions These results suggest that low‐dose methylphenidate alters the reward system (ie, increases intracranial self‐stimulation responding) in wild‐type mice via dopamine transporter inhibition, whereas dopamine transporter knockout mice do not exhibit such alterations. High‐dose methylphenidate appears to suppress intracranial self‐stimulation responding not through dopamine transporter inhibition but rather through other mechanisms. These results support the possibility that methylphenidate treatment for attention‐deficit/hyperactivity disorder does not increase the risk of drug dependence, in attention‐deficit/hyperactivity disorder patients with dopamine transporter dysfunction. |
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issn | 2574-173X |
language | English |
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publishDate | 2018-09-01 |
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spelling | doaj.art-549a64fffcf04384a637ec1ef3bb53582022-12-22T03:49:41ZengWileyNeuropsychopharmacology Reports2574-173X2018-09-0138314915310.1002/npr2.12020Reward‐enhancing effect of methylphenidate is abolished in dopamine transporter knockout mice: A model of attention‐deficit/hyperactivity disorderSoichiro Ide0Yuiko Ikekubo1Jennifer Hua2Yukio Takamatsu3George R. Uhl4Ichiro Sora5Kazutaka Ikeda6Addictive Substance Project Tokyo Metropolitan Institute of Medical Science Tokyo JapanAddictive Substance Project Tokyo Metropolitan Institute of Medical Science Tokyo JapanAddictive Substance Project Tokyo Metropolitan Institute of Medical Science Tokyo JapanAddictive Substance Project Tokyo Metropolitan Institute of Medical Science Tokyo JapanBranch of Molecular Neurobiology National Institute on Drug Abuse Baltimore MarylandAddictive Substance Project Tokyo Metropolitan Institute of Medical Science Tokyo JapanAddictive Substance Project Tokyo Metropolitan Institute of Medical Science Tokyo JapanAbstract Aim Attention‐deficit/hyperactivity disorder is a heterogeneous neurobiological disorder that is characterized by inattention, impulsivity, and an increase in motor activity. Although methylphenidate has been used as a medication for decades, unknown is whether methylphenidate treatment can cause drug dependence in patients with attention‐deficit/hyperactivity disorder. This study investigated the reward‐enhancing effects of methylphenidate using intracranial self‐stimulation in an animal model of attention‐deficit/hyperactivity disorder, dopamine transporter knockout mice. Methods For the intracranial self‐stimulation procedures, the mice were trained to nosepoke to receive direct electrical stimulation via an electrode that was implanted in the lateral hypothalamus. After the acquisition of nosepoke responding for intracranial self‐stimulation, the effects of methylphenidate on intracranial self‐stimulation were investigated. Results In the progressive‐ratio procedure, dopamine transporter knockout mice exhibited an increase in intracranial self‐stimulation compared with wild‐type mice. Treatment with 5 and 10 mg/kg methylphenidate increased intracranial self‐stimulation responding in wild‐type mice. Methylphenidate at the same doses did not affect intracranial self‐stimulation responding in dopamine transporter knockout mice. We then investigated the effects of high‐dose methylphenidate (60 mg/kg) in a rate‐frequency procedure. High‐dose methylphenidate significantly decreased intracranial self‐stimulation responding in both wild‐type and dopamine transporter knockout mice. Conclusions These results suggest that low‐dose methylphenidate alters the reward system (ie, increases intracranial self‐stimulation responding) in wild‐type mice via dopamine transporter inhibition, whereas dopamine transporter knockout mice do not exhibit such alterations. High‐dose methylphenidate appears to suppress intracranial self‐stimulation responding not through dopamine transporter inhibition but rather through other mechanisms. These results support the possibility that methylphenidate treatment for attention‐deficit/hyperactivity disorder does not increase the risk of drug dependence, in attention‐deficit/hyperactivity disorder patients with dopamine transporter dysfunction.https://doi.org/10.1002/npr2.12020attention‐deficit/hyperactivity disorderdopamine transporterintracranial self‐stimulationmethylphenidatereward |
spellingShingle | Soichiro Ide Yuiko Ikekubo Jennifer Hua Yukio Takamatsu George R. Uhl Ichiro Sora Kazutaka Ikeda Reward‐enhancing effect of methylphenidate is abolished in dopamine transporter knockout mice: A model of attention‐deficit/hyperactivity disorder Neuropsychopharmacology Reports attention‐deficit/hyperactivity disorder dopamine transporter intracranial self‐stimulation methylphenidate reward |
title | Reward‐enhancing effect of methylphenidate is abolished in dopamine transporter knockout mice: A model of attention‐deficit/hyperactivity disorder |
title_full | Reward‐enhancing effect of methylphenidate is abolished in dopamine transporter knockout mice: A model of attention‐deficit/hyperactivity disorder |
title_fullStr | Reward‐enhancing effect of methylphenidate is abolished in dopamine transporter knockout mice: A model of attention‐deficit/hyperactivity disorder |
title_full_unstemmed | Reward‐enhancing effect of methylphenidate is abolished in dopamine transporter knockout mice: A model of attention‐deficit/hyperactivity disorder |
title_short | Reward‐enhancing effect of methylphenidate is abolished in dopamine transporter knockout mice: A model of attention‐deficit/hyperactivity disorder |
title_sort | reward enhancing effect of methylphenidate is abolished in dopamine transporter knockout mice a model of attention deficit hyperactivity disorder |
topic | attention‐deficit/hyperactivity disorder dopamine transporter intracranial self‐stimulation methylphenidate reward |
url | https://doi.org/10.1002/npr2.12020 |
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