Chromatin Accessibility and Transcriptomic Alterations in Murine Ovarian Granulosa Cells upon Deoxynivalenol Exposure
Deoxynivalenol (DON) is a common environmental toxin that is secreted by fusarium fungi that frequently contaminates feedstuff and food. While the detrimental effects of DON on human and animal reproductive systems have been well recognized, the underlying mechanism remains poorly understood. Ovaria...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/10/11/2818 |
| _version_ | 1797510858694197248 |
|---|---|
| author | Hairui Fan Zhanshi Ren Chao Xu Haifei Wang Zhengchang Wu Zia ur Rehman Shenglong Wu Ming-an Sun Wenbin Bao |
| author_facet | Hairui Fan Zhanshi Ren Chao Xu Haifei Wang Zhengchang Wu Zia ur Rehman Shenglong Wu Ming-an Sun Wenbin Bao |
| author_sort | Hairui Fan |
| collection | DOAJ |
| description | Deoxynivalenol (DON) is a common environmental toxin that is secreted by fusarium fungi that frequently contaminates feedstuff and food. While the detrimental effects of DON on human and animal reproductive systems have been well recognized, the underlying mechanism remains poorly understood. Ovarian granulosa cells (GCs), which surround oocytes, are crucial for regulating oocyte development, mainly through the secretion of hormones such as estrogen and progesterone. Using an in vitro model of murine GCs, we characterized the cytotoxic effects of DON and profiled genome-wide chromatin accessibility and transcriptomic alterations after DON exposure. Our results suggest that DON can induce decreased viability and growth, increased apoptosis rate, and disrupted hormone secretion. In total, 2533 differentially accessible loci and 2675 differentially expressed genes were identified that were associated with Hippo, Wnt, steroid biosynthesis, sulfur metabolism, and inflammation-related pathways. DON-induced genes usually have a concurrently increased occupancy of active histone modifications H3K4me3 and H3K27ac in their promoters. Integrative analyses identified 35 putative directly affected genes including Adrb2 and Fshr, which are key regulators of follicular growth, and revealed that regions with increased chromatin accessibility are enriched with the binding motifs for NR5A1 and NR5A2, which are important for GCs. Moreover, DON-induced inflammatory response is due to the activation of the NF-κB and MAPK signaling pathways. Overall, our results provide novel insights into the regulatory elements, genes, and key pathways underlying the response of ovarian GCs to DON cytotoxicity. |
| first_indexed | 2024-03-10T05:37:17Z |
| format | Article |
| id | doaj.art-549d3028d7c4412681059a4d8b72b4bd |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T05:37:17Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-549d3028d7c4412681059a4d8b72b4bd2023-11-22T22:47:11ZengMDPI AGCells2073-44092021-10-011011281810.3390/cells10112818Chromatin Accessibility and Transcriptomic Alterations in Murine Ovarian Granulosa Cells upon Deoxynivalenol ExposureHairui Fan0Zhanshi Ren1Chao Xu2Haifei Wang3Zhengchang Wu4Zia ur Rehman5Shenglong Wu6Ming-an Sun7Wenbin Bao8Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaFaculty of Animal Husbandry and Veterinary Sciences, College of Veterinary Sciences, The University of Agriculture Peshawar, Peshawar 25000, PakistanKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaInstitute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaDeoxynivalenol (DON) is a common environmental toxin that is secreted by fusarium fungi that frequently contaminates feedstuff and food. While the detrimental effects of DON on human and animal reproductive systems have been well recognized, the underlying mechanism remains poorly understood. Ovarian granulosa cells (GCs), which surround oocytes, are crucial for regulating oocyte development, mainly through the secretion of hormones such as estrogen and progesterone. Using an in vitro model of murine GCs, we characterized the cytotoxic effects of DON and profiled genome-wide chromatin accessibility and transcriptomic alterations after DON exposure. Our results suggest that DON can induce decreased viability and growth, increased apoptosis rate, and disrupted hormone secretion. In total, 2533 differentially accessible loci and 2675 differentially expressed genes were identified that were associated with Hippo, Wnt, steroid biosynthesis, sulfur metabolism, and inflammation-related pathways. DON-induced genes usually have a concurrently increased occupancy of active histone modifications H3K4me3 and H3K27ac in their promoters. Integrative analyses identified 35 putative directly affected genes including Adrb2 and Fshr, which are key regulators of follicular growth, and revealed that regions with increased chromatin accessibility are enriched with the binding motifs for NR5A1 and NR5A2, which are important for GCs. Moreover, DON-induced inflammatory response is due to the activation of the NF-κB and MAPK signaling pathways. Overall, our results provide novel insights into the regulatory elements, genes, and key pathways underlying the response of ovarian GCs to DON cytotoxicity.https://www.mdpi.com/2073-4409/10/11/2818ovarian granulosa cellsmycotoxinchromatin accessibilitygene expressioncytotoxicity |
| spellingShingle | Hairui Fan Zhanshi Ren Chao Xu Haifei Wang Zhengchang Wu Zia ur Rehman Shenglong Wu Ming-an Sun Wenbin Bao Chromatin Accessibility and Transcriptomic Alterations in Murine Ovarian Granulosa Cells upon Deoxynivalenol Exposure Cells ovarian granulosa cells mycotoxin chromatin accessibility gene expression cytotoxicity |
| title | Chromatin Accessibility and Transcriptomic Alterations in Murine Ovarian Granulosa Cells upon Deoxynivalenol Exposure |
| title_full | Chromatin Accessibility and Transcriptomic Alterations in Murine Ovarian Granulosa Cells upon Deoxynivalenol Exposure |
| title_fullStr | Chromatin Accessibility and Transcriptomic Alterations in Murine Ovarian Granulosa Cells upon Deoxynivalenol Exposure |
| title_full_unstemmed | Chromatin Accessibility and Transcriptomic Alterations in Murine Ovarian Granulosa Cells upon Deoxynivalenol Exposure |
| title_short | Chromatin Accessibility and Transcriptomic Alterations in Murine Ovarian Granulosa Cells upon Deoxynivalenol Exposure |
| title_sort | chromatin accessibility and transcriptomic alterations in murine ovarian granulosa cells upon deoxynivalenol exposure |
| topic | ovarian granulosa cells mycotoxin chromatin accessibility gene expression cytotoxicity |
| url | https://www.mdpi.com/2073-4409/10/11/2818 |
| work_keys_str_mv | AT hairuifan chromatinaccessibilityandtranscriptomicalterationsinmurineovariangranulosacellsupondeoxynivalenolexposure AT zhanshiren chromatinaccessibilityandtranscriptomicalterationsinmurineovariangranulosacellsupondeoxynivalenolexposure AT chaoxu chromatinaccessibilityandtranscriptomicalterationsinmurineovariangranulosacellsupondeoxynivalenolexposure AT haifeiwang chromatinaccessibilityandtranscriptomicalterationsinmurineovariangranulosacellsupondeoxynivalenolexposure AT zhengchangwu chromatinaccessibilityandtranscriptomicalterationsinmurineovariangranulosacellsupondeoxynivalenolexposure AT ziaurrehman chromatinaccessibilityandtranscriptomicalterationsinmurineovariangranulosacellsupondeoxynivalenolexposure AT shenglongwu chromatinaccessibilityandtranscriptomicalterationsinmurineovariangranulosacellsupondeoxynivalenolexposure AT mingansun chromatinaccessibilityandtranscriptomicalterationsinmurineovariangranulosacellsupondeoxynivalenolexposure AT wenbinbao chromatinaccessibilityandtranscriptomicalterationsinmurineovariangranulosacellsupondeoxynivalenolexposure |