Effect of salinity, nitrogen and phosphorus stresses on growth and photosynthetic activity of the marine microalga Dunaliella parva
The growth of the marine green alga Dunaliella parva was studied and optimized under different salinity levels of NaCl (0.5, 1, 2, 2.5, and 3.5 M). The growth was monitored by cell number pigment content (Chl. a, Chl. b, and carotenoids). The grown alga, under the optimal conditions, was exposed to...
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| Format: | Article |
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AcademicPres
2024-03-01
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| Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
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| Online Access: | https://www.notulaebotanicae.ro/index.php/nbha/article/view/13426 |
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| author | Mostafa EL-SHEEKH Samha DEWIDAR Azza HAMAD Abdelghafar M. ABU-ELSAOUD Maha ALHARBI Amr ELKELISH |
| author_facet | Mostafa EL-SHEEKH Samha DEWIDAR Azza HAMAD Abdelghafar M. ABU-ELSAOUD Maha ALHARBI Amr ELKELISH |
| author_sort | Mostafa EL-SHEEKH |
| collection | DOAJ |
| description |
The growth of the marine green alga Dunaliella parva was studied and optimized under different salinity levels of NaCl (0.5, 1, 2, 2.5, and 3.5 M). The growth was monitored by cell number pigment content (Chl. a, Chl. b, and carotenoids). The grown alga, under the optimal conditions, was exposed to different stresses (nitrogen, phosphorus starvation, and salinity either singly or combined. Under nitrogen and phosphorus starvation, either singly or combined, the growth rate and the metabolic activities were decreased. Under salt stress (2.5 M NaCl) combined with N starvation and heavy metals stress, glycerol production increased, while glycerol synthesis decreased under salt stress of 1 M NaCl and P starvation. Also, free radicals (total antioxidant, reducing power, DPPH, and Lipid peroxidation), pigment content, and activity of antioxidant enzymes were recorded. D. parva grown under salinity level (2.5 M NaCl) combined with nutrient starvation correlated with more efficient enzymatic antioxidant activity accumulation. This study strongly suggested that the induction of antioxidant defense was one component of the tolerance mechanism of D. parva to salinity, as evidenced by its growth behavior.
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| first_indexed | 2024-04-24T15:42:42Z |
| format | Article |
| id | doaj.art-549d8ede4a344764877b45d1d14784f7 |
| institution | Directory Open Access Journal |
| issn | 0255-965X 1842-4309 |
| language | English |
| last_indexed | 2024-04-24T15:42:42Z |
| publishDate | 2024-03-01 |
| publisher | AcademicPres |
| record_format | Article |
| series | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| spelling | doaj.art-549d8ede4a344764877b45d1d14784f72024-04-01T18:49:53ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092024-03-0152110.15835/nbha52113426Effect of salinity, nitrogen and phosphorus stresses on growth and photosynthetic activity of the marine microalga Dunaliella parvaMostafa EL-SHEEKH0Samha DEWIDAR1Azza HAMAD2Abdelghafar M. ABU-ELSAOUD3Maha ALHARBI4Amr ELKELISH5Tanta University, Faculty of Science, Botany Department, Tanta 31527Tanta University, Faculty of Science, Botany Department, Tanta 31527Tanta University, Faculty of Science, Botany Department, Tanta 31527Imam Muhammad bin Saud Islamic University (IMSIU), College of Science, Department of Biology, Riyadh 11623; Suez Canal University, Faculty of Science, Department of Botany and Microbiology, Ismailia, 41522Princess Nourah bint Abdulrahman University, College of Science, Department of Biology, P.O. Box 84428, Riyadh 11671Imam Muhammad bin Saud Islamic University (IMSIU), College of Science, Department of Biology, Riyadh 11623; Suez Canal University, Faculty of Science, Department of Botany and Microbiology, Ismailia, 41522 The growth of the marine green alga Dunaliella parva was studied and optimized under different salinity levels of NaCl (0.5, 1, 2, 2.5, and 3.5 M). The growth was monitored by cell number pigment content (Chl. a, Chl. b, and carotenoids). The grown alga, under the optimal conditions, was exposed to different stresses (nitrogen, phosphorus starvation, and salinity either singly or combined. Under nitrogen and phosphorus starvation, either singly or combined, the growth rate and the metabolic activities were decreased. Under salt stress (2.5 M NaCl) combined with N starvation and heavy metals stress, glycerol production increased, while glycerol synthesis decreased under salt stress of 1 M NaCl and P starvation. Also, free radicals (total antioxidant, reducing power, DPPH, and Lipid peroxidation), pigment content, and activity of antioxidant enzymes were recorded. D. parva grown under salinity level (2.5 M NaCl) combined with nutrient starvation correlated with more efficient enzymatic antioxidant activity accumulation. This study strongly suggested that the induction of antioxidant defense was one component of the tolerance mechanism of D. parva to salinity, as evidenced by its growth behavior. https://www.notulaebotanicae.ro/index.php/nbha/article/view/13426antioxidant enzymesDunaliella parvaphotosynthesissalinity |
| spellingShingle | Mostafa EL-SHEEKH Samha DEWIDAR Azza HAMAD Abdelghafar M. ABU-ELSAOUD Maha ALHARBI Amr ELKELISH Effect of salinity, nitrogen and phosphorus stresses on growth and photosynthetic activity of the marine microalga Dunaliella parva Notulae Botanicae Horti Agrobotanici Cluj-Napoca antioxidant enzymes Dunaliella parva photosynthesis salinity |
| title | Effect of salinity, nitrogen and phosphorus stresses on growth and photosynthetic activity of the marine microalga Dunaliella parva |
| title_full | Effect of salinity, nitrogen and phosphorus stresses on growth and photosynthetic activity of the marine microalga Dunaliella parva |
| title_fullStr | Effect of salinity, nitrogen and phosphorus stresses on growth and photosynthetic activity of the marine microalga Dunaliella parva |
| title_full_unstemmed | Effect of salinity, nitrogen and phosphorus stresses on growth and photosynthetic activity of the marine microalga Dunaliella parva |
| title_short | Effect of salinity, nitrogen and phosphorus stresses on growth and photosynthetic activity of the marine microalga Dunaliella parva |
| title_sort | effect of salinity nitrogen and phosphorus stresses on growth and photosynthetic activity of the marine microalga dunaliella parva |
| topic | antioxidant enzymes Dunaliella parva photosynthesis salinity |
| url | https://www.notulaebotanicae.ro/index.php/nbha/article/view/13426 |
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