Isolation and molecular characterization of the Salmonella Typhimurium orphan phage Arash
Abstract The current threat of multidrug resistant strains necessitates development of alternatives to antibiotics such as bacteriophages. This study describes the isolation and characterization of a novel Salmonella Typhimurium phage ‘Arash’ from hospital wastewater in Leuven, Belgium. Arash has a...
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-03056-9 |
| _version_ | 1797453689418416128 |
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| author | Mohammad Hashem Yousefi Jeroen Wagemans Seyed Shahram Shekarforoush Marta Vallino Nadiia Pozhydaieva Katharina Höfer Rob Lavigne Saeid Hosseinzadeh |
| author_facet | Mohammad Hashem Yousefi Jeroen Wagemans Seyed Shahram Shekarforoush Marta Vallino Nadiia Pozhydaieva Katharina Höfer Rob Lavigne Saeid Hosseinzadeh |
| author_sort | Mohammad Hashem Yousefi |
| collection | DOAJ |
| description | Abstract The current threat of multidrug resistant strains necessitates development of alternatives to antibiotics such as bacteriophages. This study describes the isolation and characterization of a novel Salmonella Typhimurium phage ‘Arash’ from hospital wastewater in Leuven, Belgium. Arash has a myovirus morphology with a 95 nm capsid and a 140 nm tail. The host range of Arash is restricted to its isolation host. Approximately 86% of the phage particles are adsorbed to a host cell within 10 min. Arash has latent period of 65 min and burst size of 425 PFU/cell. Arash has a dsDNA genome of 180,819 bp with GC content of 53.02% with no similarities to any characterized phages, suggesting Arash as a novel species in the novel ‘Arashvirus’ genus. Arash carries no apparent lysogeny-, antibiotic resistance- nor virulence-related genes. Proteome analysis revealed 116 proteins as part of the mature phage particles of which 27 could be assigned a function. Therefore, the present findings shed light on the morphological, microbiological and genomic characteristics of Arash and suggest its potential application as therapeutic and/or biocontrol agent. |
| first_indexed | 2024-03-09T15:26:14Z |
| format | Article |
| id | doaj.art-549e87b1506a41a5a8a433bb13c4db7c |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-09T15:26:14Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-549e87b1506a41a5a8a433bb13c4db7c2023-11-26T12:30:21ZengBMCBMC Microbiology1471-21802023-10-0123111210.1186/s12866-023-03056-9Isolation and molecular characterization of the Salmonella Typhimurium orphan phage ArashMohammad Hashem Yousefi0Jeroen Wagemans1Seyed Shahram Shekarforoush2Marta Vallino3Nadiia Pozhydaieva4Katharina Höfer5Rob Lavigne6Saeid Hosseinzadeh7Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz UniversityDepartment of Biosystems, KU LeuvenDepartment of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz UniversityInstitute of Sustainable Plant Protection, National Research Council of ItalyMax Planck Institute for Terrestrial Microbiology, SYNMIKROMax Planck Institute for Terrestrial Microbiology, SYNMIKRODepartment of Biosystems, KU LeuvenDepartment of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz UniversityAbstract The current threat of multidrug resistant strains necessitates development of alternatives to antibiotics such as bacteriophages. This study describes the isolation and characterization of a novel Salmonella Typhimurium phage ‘Arash’ from hospital wastewater in Leuven, Belgium. Arash has a myovirus morphology with a 95 nm capsid and a 140 nm tail. The host range of Arash is restricted to its isolation host. Approximately 86% of the phage particles are adsorbed to a host cell within 10 min. Arash has latent period of 65 min and burst size of 425 PFU/cell. Arash has a dsDNA genome of 180,819 bp with GC content of 53.02% with no similarities to any characterized phages, suggesting Arash as a novel species in the novel ‘Arashvirus’ genus. Arash carries no apparent lysogeny-, antibiotic resistance- nor virulence-related genes. Proteome analysis revealed 116 proteins as part of the mature phage particles of which 27 could be assigned a function. Therefore, the present findings shed light on the morphological, microbiological and genomic characteristics of Arash and suggest its potential application as therapeutic and/or biocontrol agent.https://doi.org/10.1186/s12866-023-03056-9BacteriophageSalmonella TyphimuriumNovel phage genus‘Arashvirus’ |
| spellingShingle | Mohammad Hashem Yousefi Jeroen Wagemans Seyed Shahram Shekarforoush Marta Vallino Nadiia Pozhydaieva Katharina Höfer Rob Lavigne Saeid Hosseinzadeh Isolation and molecular characterization of the Salmonella Typhimurium orphan phage Arash BMC Microbiology Bacteriophage Salmonella Typhimurium Novel phage genus ‘Arashvirus’ |
| title | Isolation and molecular characterization of the Salmonella Typhimurium orphan phage Arash |
| title_full | Isolation and molecular characterization of the Salmonella Typhimurium orphan phage Arash |
| title_fullStr | Isolation and molecular characterization of the Salmonella Typhimurium orphan phage Arash |
| title_full_unstemmed | Isolation and molecular characterization of the Salmonella Typhimurium orphan phage Arash |
| title_short | Isolation and molecular characterization of the Salmonella Typhimurium orphan phage Arash |
| title_sort | isolation and molecular characterization of the salmonella typhimurium orphan phage arash |
| topic | Bacteriophage Salmonella Typhimurium Novel phage genus ‘Arashvirus’ |
| url | https://doi.org/10.1186/s12866-023-03056-9 |
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