Correlation between auto/mitophagic processes and magnetic resonance imaging activity in multiple sclerosis patients
Abstract Background An alteration of autophagy and mitophagy, two highly conserved lysosome-dependent degradation pathways involved in the maintenance of cellular homeostasis, has been associated with multiple sclerosis (MS). Objective To search the level of autophagy-related 5 (ATG5) and Parkin pro...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-06-01
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| Series: | Journal of Neuroinflammation |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12974-019-1526-0 |
| _version_ | 1819048075524571136 |
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| author | Massimiliano Castellazzi Simone Patergnani Mariapina Donadio Carlotta Giorgi Massimo Bonora Enrico Fainardi Ilaria Casetta Enrico Granieri Maura Pugliatti Paolo Pinton |
| author_facet | Massimiliano Castellazzi Simone Patergnani Mariapina Donadio Carlotta Giorgi Massimo Bonora Enrico Fainardi Ilaria Casetta Enrico Granieri Maura Pugliatti Paolo Pinton |
| author_sort | Massimiliano Castellazzi |
| collection | DOAJ |
| description | Abstract Background An alteration of autophagy and mitophagy, two highly conserved lysosome-dependent degradation pathways involved in the maintenance of cellular homeostasis, has been associated with multiple sclerosis (MS). Objective To search the level of autophagy-related 5 (ATG5) and Parkin proteins, as markers of autophagy and mitophagy respectively, and lactate in a cohort of MS patients. Methods Cerebrospinal fluid (CSF) and serum samples from 60 MS patients were analyzed: 30 with magnetic resonance imaging (MRI) evidence of disease activity, gadolinium (Gd)-based contrast agent positive (Gd+), and 30 without MRI evidence of disease activity (Gd−). ATG5, Parkin, and lactate were measured using commercially available products. Results and conclusions Serum levels of ATG5, Parkin, and lactate were more elevated in Gd+ than in Gd− MS patients (p < 0.0001), and CSF concentrations of ATG5 and Parkin were greater in Gd+ than in Gd− MS (p < 0.0001). Our results demonstrated that molecular markers of autophagy and mitophagy are increased in CSF of MS patients during the active phases of the disease and that these catabolic markers, together with lactate, are also remarkably augmented in blood suggesting a role of these processes in MS pathogenesis and the possible use of these molecules as biomarkers of disease activity. |
| first_indexed | 2024-12-21T11:10:29Z |
| format | Article |
| id | doaj.art-54a083977a944484b4169f01e7824503 |
| institution | Directory Open Access Journal |
| issn | 1742-2094 |
| language | English |
| last_indexed | 2024-12-21T11:10:29Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Neuroinflammation |
| spelling | doaj.art-54a083977a944484b4169f01e78245032022-12-21T19:06:06ZengBMCJournal of Neuroinflammation1742-20942019-06-011611810.1186/s12974-019-1526-0Correlation between auto/mitophagic processes and magnetic resonance imaging activity in multiple sclerosis patientsMassimiliano Castellazzi0Simone Patergnani1Mariapina Donadio2Carlotta Giorgi3Massimo Bonora4Enrico Fainardi5Ilaria Casetta6Enrico Granieri7Maura Pugliatti8Paolo Pinton9Department of Biomedical and Specialist Surgical Sciences, Section of Neurological, Psychiatric and Psychological Sciences, University of FerraraDepartment of Morphology, Surgery and Experimental Medicine, Section of Pathology, Oncology and Experimental Biology, Laboratory for Technologies of Advanced Therapies (LTTA), University of FerraraDepartment of Biomedical and Specialist Surgical Sciences, Section of Neurological, Psychiatric and Psychological Sciences, University of FerraraDepartment of Morphology, Surgery and Experimental Medicine, Section of Pathology, Oncology and Experimental Biology, Laboratory for Technologies of Advanced Therapies (LTTA), University of FerraraDepartment of Morphology, Surgery and Experimental Medicine, Section of Pathology, Oncology and Experimental Biology, Laboratory for Technologies of Advanced Therapies (LTTA), University of FerraraInterdepartmental Research Center for the Study of Multiple Sclerosis and Inflammatory and Degenerative Diseases of the Nervous System, University of FerraraDepartment of Biomedical and Specialist Surgical Sciences, Section of Neurological, Psychiatric and Psychological Sciences, University of FerraraDepartment of Biomedical and Specialist Surgical Sciences, Section of Neurological, Psychiatric and Psychological Sciences, University of FerraraDepartment of Biomedical and Specialist Surgical Sciences, Section of Neurological, Psychiatric and Psychological Sciences, University of FerraraDepartment of Morphology, Surgery and Experimental Medicine, Section of Pathology, Oncology and Experimental Biology, Laboratory for Technologies of Advanced Therapies (LTTA), University of FerraraAbstract Background An alteration of autophagy and mitophagy, two highly conserved lysosome-dependent degradation pathways involved in the maintenance of cellular homeostasis, has been associated with multiple sclerosis (MS). Objective To search the level of autophagy-related 5 (ATG5) and Parkin proteins, as markers of autophagy and mitophagy respectively, and lactate in a cohort of MS patients. Methods Cerebrospinal fluid (CSF) and serum samples from 60 MS patients were analyzed: 30 with magnetic resonance imaging (MRI) evidence of disease activity, gadolinium (Gd)-based contrast agent positive (Gd+), and 30 without MRI evidence of disease activity (Gd−). ATG5, Parkin, and lactate were measured using commercially available products. Results and conclusions Serum levels of ATG5, Parkin, and lactate were more elevated in Gd+ than in Gd− MS patients (p < 0.0001), and CSF concentrations of ATG5 and Parkin were greater in Gd+ than in Gd− MS (p < 0.0001). Our results demonstrated that molecular markers of autophagy and mitophagy are increased in CSF of MS patients during the active phases of the disease and that these catabolic markers, together with lactate, are also remarkably augmented in blood suggesting a role of these processes in MS pathogenesis and the possible use of these molecules as biomarkers of disease activity.http://link.springer.com/article/10.1186/s12974-019-1526-0Multiple sclerosisAutophagyMitophagyBiomarkersMagnetic resonance imaging |
| spellingShingle | Massimiliano Castellazzi Simone Patergnani Mariapina Donadio Carlotta Giorgi Massimo Bonora Enrico Fainardi Ilaria Casetta Enrico Granieri Maura Pugliatti Paolo Pinton Correlation between auto/mitophagic processes and magnetic resonance imaging activity in multiple sclerosis patients Journal of Neuroinflammation Multiple sclerosis Autophagy Mitophagy Biomarkers Magnetic resonance imaging |
| title | Correlation between auto/mitophagic processes and magnetic resonance imaging activity in multiple sclerosis patients |
| title_full | Correlation between auto/mitophagic processes and magnetic resonance imaging activity in multiple sclerosis patients |
| title_fullStr | Correlation between auto/mitophagic processes and magnetic resonance imaging activity in multiple sclerosis patients |
| title_full_unstemmed | Correlation between auto/mitophagic processes and magnetic resonance imaging activity in multiple sclerosis patients |
| title_short | Correlation between auto/mitophagic processes and magnetic resonance imaging activity in multiple sclerosis patients |
| title_sort | correlation between auto mitophagic processes and magnetic resonance imaging activity in multiple sclerosis patients |
| topic | Multiple sclerosis Autophagy Mitophagy Biomarkers Magnetic resonance imaging |
| url | http://link.springer.com/article/10.1186/s12974-019-1526-0 |
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