Comparative transcriptomic analyses of chlorogenic acid and luteolosides biosynthesis pathways at different flowering stages of diploid and tetraploid Lonicera japonica
The Flos Lonicerae Japonicae (FLJ), Lonicera japonica Thunb, belonging to the Caprifoliaceae family, is an economically important plant that is highly utilized in traditional Chinese medicine as well as in Japanese medicine. The flowers of these plants are rich in chlorogenic acid (CGA) and luteolos...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2020-03-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/8690.pdf |
| _version_ | 1797418180715479040 |
|---|---|
| author | Hongli Wang Yanqun Li Sibo Wang Dexin Kong Sunil Kumar Sahu Mei Bai Haoyuan Li Linzhou Li Yan Xu Hongping Liang Huan Liu Hong Wu |
| author_facet | Hongli Wang Yanqun Li Sibo Wang Dexin Kong Sunil Kumar Sahu Mei Bai Haoyuan Li Linzhou Li Yan Xu Hongping Liang Huan Liu Hong Wu |
| author_sort | Hongli Wang |
| collection | DOAJ |
| description | The Flos Lonicerae Japonicae (FLJ), Lonicera japonica Thunb, belonging to the Caprifoliaceae family, is an economically important plant that is highly utilized in traditional Chinese medicine as well as in Japanese medicine. The flowers of these plants are rich in chlorogenic acid (CGA) and luteoloside. Our previous study revealed that tetraploid L. japonica has higher fresh/dry weight, phenolic acids and flavonoids contents than those of diploid plants. However, why tetraploid L. japonica can yield higher CGA and luteolosides than that in diploid and what is the difference in the molecular regulatory mechanism of these pathways between diploid and tetraploids remained unclear. Therefore, in the present study, we performed comprehensive transcriptome analyses of different flowering stages of diploid and tetraploid L. japonica. The CGA content of tetraploid was found higher than that of diploid at all the growth stages. While the luteoloside content of diploid was higher than that of tetraploid at S4 and S6 growth stages. We obtained a high-quality transcriptome assembly (N50 = 2,055 bp; Average length = 1,331 bp) compared to earlier studies. Differential expression analysis revealed that several important genes involving in plant hormone signal transduction, carbon metabolism, starch/sucrose metabolism and plant-pathogen interaction were upregulated in tetraploid compared with the diploid L. japonica, reflecting the higher adaptability and resistance of tetraploid species. Furthermore, by associating the phenotypic data and gene expression profiles, we were able to characterize the potential molecular regulatory mechanism of important biosynthetic pathways at different flowering stages. Overall, our work provides a foundation for further research on these important secondary metabolite pathways and their implications in traditional Chinese/Japanese medicine. |
| first_indexed | 2024-03-09T06:28:47Z |
| format | Article |
| id | doaj.art-54a0fabd203d470ebec056e27864fda0 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:28:47Z |
| publishDate | 2020-03-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-54a0fabd203d470ebec056e27864fda02023-12-03T11:10:38ZengPeerJ Inc.PeerJ2167-83592020-03-018e869010.7717/peerj.8690Comparative transcriptomic analyses of chlorogenic acid and luteolosides biosynthesis pathways at different flowering stages of diploid and tetraploid Lonicera japonicaHongli Wang0Yanqun Li1Sibo Wang2Dexin Kong3Sunil Kumar Sahu4Mei Bai5Haoyuan Li6Linzhou Li7Yan Xu8Hongping Liang9Huan Liu10Hong Wu11BGI-Shenzhen, Shenzhen, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, Guangdong, ChinaBGI-Shenzhen, Shenzhen, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, Guangdong, ChinaBGI-Shenzhen, Shenzhen, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, Guangdong, ChinaBGI-Shenzhen, Shenzhen, Guangdong, ChinaBGI-Shenzhen, Shenzhen, Guangdong, ChinaBGI-Shenzhen, Shenzhen, Guangdong, ChinaBGI-Shenzhen, Shenzhen, Guangdong, ChinaBGI-Shenzhen, Shenzhen, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, Guangdong, ChinaThe Flos Lonicerae Japonicae (FLJ), Lonicera japonica Thunb, belonging to the Caprifoliaceae family, is an economically important plant that is highly utilized in traditional Chinese medicine as well as in Japanese medicine. The flowers of these plants are rich in chlorogenic acid (CGA) and luteoloside. Our previous study revealed that tetraploid L. japonica has higher fresh/dry weight, phenolic acids and flavonoids contents than those of diploid plants. However, why tetraploid L. japonica can yield higher CGA and luteolosides than that in diploid and what is the difference in the molecular regulatory mechanism of these pathways between diploid and tetraploids remained unclear. Therefore, in the present study, we performed comprehensive transcriptome analyses of different flowering stages of diploid and tetraploid L. japonica. The CGA content of tetraploid was found higher than that of diploid at all the growth stages. While the luteoloside content of diploid was higher than that of tetraploid at S4 and S6 growth stages. We obtained a high-quality transcriptome assembly (N50 = 2,055 bp; Average length = 1,331 bp) compared to earlier studies. Differential expression analysis revealed that several important genes involving in plant hormone signal transduction, carbon metabolism, starch/sucrose metabolism and plant-pathogen interaction were upregulated in tetraploid compared with the diploid L. japonica, reflecting the higher adaptability and resistance of tetraploid species. Furthermore, by associating the phenotypic data and gene expression profiles, we were able to characterize the potential molecular regulatory mechanism of important biosynthetic pathways at different flowering stages. Overall, our work provides a foundation for further research on these important secondary metabolite pathways and their implications in traditional Chinese/Japanese medicine.https://peerj.com/articles/8690.pdfLonicera japonicaDe novo transcriptome assemblyChlorogenic acidLuteolosidesTraditional Chinese medicineFlower development |
| spellingShingle | Hongli Wang Yanqun Li Sibo Wang Dexin Kong Sunil Kumar Sahu Mei Bai Haoyuan Li Linzhou Li Yan Xu Hongping Liang Huan Liu Hong Wu Comparative transcriptomic analyses of chlorogenic acid and luteolosides biosynthesis pathways at different flowering stages of diploid and tetraploid Lonicera japonica PeerJ Lonicera japonica De novo transcriptome assembly Chlorogenic acid Luteolosides Traditional Chinese medicine Flower development |
| title | Comparative transcriptomic analyses of chlorogenic acid and luteolosides biosynthesis pathways at different flowering stages of diploid and tetraploid Lonicera japonica |
| title_full | Comparative transcriptomic analyses of chlorogenic acid and luteolosides biosynthesis pathways at different flowering stages of diploid and tetraploid Lonicera japonica |
| title_fullStr | Comparative transcriptomic analyses of chlorogenic acid and luteolosides biosynthesis pathways at different flowering stages of diploid and tetraploid Lonicera japonica |
| title_full_unstemmed | Comparative transcriptomic analyses of chlorogenic acid and luteolosides biosynthesis pathways at different flowering stages of diploid and tetraploid Lonicera japonica |
| title_short | Comparative transcriptomic analyses of chlorogenic acid and luteolosides biosynthesis pathways at different flowering stages of diploid and tetraploid Lonicera japonica |
| title_sort | comparative transcriptomic analyses of chlorogenic acid and luteolosides biosynthesis pathways at different flowering stages of diploid and tetraploid lonicera japonica |
| topic | Lonicera japonica De novo transcriptome assembly Chlorogenic acid Luteolosides Traditional Chinese medicine Flower development |
| url | https://peerj.com/articles/8690.pdf |
| work_keys_str_mv | AT hongliwang comparativetranscriptomicanalysesofchlorogenicacidandluteolosidesbiosynthesispathwaysatdifferentfloweringstagesofdiploidandtetraploidlonicerajaponica AT yanqunli comparativetranscriptomicanalysesofchlorogenicacidandluteolosidesbiosynthesispathwaysatdifferentfloweringstagesofdiploidandtetraploidlonicerajaponica AT sibowang comparativetranscriptomicanalysesofchlorogenicacidandluteolosidesbiosynthesispathwaysatdifferentfloweringstagesofdiploidandtetraploidlonicerajaponica AT dexinkong comparativetranscriptomicanalysesofchlorogenicacidandluteolosidesbiosynthesispathwaysatdifferentfloweringstagesofdiploidandtetraploidlonicerajaponica AT sunilkumarsahu comparativetranscriptomicanalysesofchlorogenicacidandluteolosidesbiosynthesispathwaysatdifferentfloweringstagesofdiploidandtetraploidlonicerajaponica AT meibai comparativetranscriptomicanalysesofchlorogenicacidandluteolosidesbiosynthesispathwaysatdifferentfloweringstagesofdiploidandtetraploidlonicerajaponica AT haoyuanli comparativetranscriptomicanalysesofchlorogenicacidandluteolosidesbiosynthesispathwaysatdifferentfloweringstagesofdiploidandtetraploidlonicerajaponica AT linzhouli comparativetranscriptomicanalysesofchlorogenicacidandluteolosidesbiosynthesispathwaysatdifferentfloweringstagesofdiploidandtetraploidlonicerajaponica AT yanxu comparativetranscriptomicanalysesofchlorogenicacidandluteolosidesbiosynthesispathwaysatdifferentfloweringstagesofdiploidandtetraploidlonicerajaponica AT hongpingliang comparativetranscriptomicanalysesofchlorogenicacidandluteolosidesbiosynthesispathwaysatdifferentfloweringstagesofdiploidandtetraploidlonicerajaponica AT huanliu comparativetranscriptomicanalysesofchlorogenicacidandluteolosidesbiosynthesispathwaysatdifferentfloweringstagesofdiploidandtetraploidlonicerajaponica AT hongwu comparativetranscriptomicanalysesofchlorogenicacidandluteolosidesbiosynthesispathwaysatdifferentfloweringstagesofdiploidandtetraploidlonicerajaponica |