A Closed-Loop Optogenetic Platform
Neuromodulation is an established treatment for numerous neurological conditions, but to expand the therapeutic scope there is a need to improve the spatial, temporal and cell-type specificity of stimulation. Optogenetics is a promising area of current research, enabling optical stimulation of genet...
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-09-01
|
| Series: | Frontiers in Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2021.718311/full |
| _version_ | 1818909994514382848 |
|---|---|
| author | Dimitrios Firfilionis Frances Hutchings Reza Tamadoni Darren Walsh Mark Turnbull Enrique Escobedo-Cousin Richard G. Bailey Johannes Gausden Aaliyah Patel Dorian Haci Yan Liu Yan Liu Fiona E. N. LeBeau Andrew Trevelyan Timothy G. Constandinou Timothy G. Constandinou Anthony O'Neill Marcus Kaiser Marcus Kaiser Marcus Kaiser Patrick Degenaar Andrew Jackson |
| author_facet | Dimitrios Firfilionis Frances Hutchings Reza Tamadoni Darren Walsh Mark Turnbull Enrique Escobedo-Cousin Richard G. Bailey Johannes Gausden Aaliyah Patel Dorian Haci Yan Liu Yan Liu Fiona E. N. LeBeau Andrew Trevelyan Timothy G. Constandinou Timothy G. Constandinou Anthony O'Neill Marcus Kaiser Marcus Kaiser Marcus Kaiser Patrick Degenaar Andrew Jackson |
| author_sort | Dimitrios Firfilionis |
| collection | DOAJ |
| description | Neuromodulation is an established treatment for numerous neurological conditions, but to expand the therapeutic scope there is a need to improve the spatial, temporal and cell-type specificity of stimulation. Optogenetics is a promising area of current research, enabling optical stimulation of genetically-defined cell types without interfering with concurrent electrical recording for closed-loop control of neural activity. We are developing an open-source system to provide a platform for closed-loop optogenetic neuromodulation, incorporating custom integrated circuitry for recording and stimulation, real-time closed-loop algorithms running on a microcontroller and experimental control via a PC interface. We include commercial components to validate performance, with the ultimate aim of translating this approach to humans. In the meantime our system is flexible and expandable for use in a variety of preclinical neuroscientific applications. The platform consists of a Controlling Abnormal Network Dynamics using Optogenetics (CANDO) Control System (CS) that interfaces with up to four CANDO headstages responsible for electrical recording and optical stimulation through custom CANDO LED optrodes. Control of the hardware, inbuilt algorithms and data acquisition is enabled via the CANDO GUI (Graphical User Interface). Here we describe the design and implementation of this system, and demonstrate how it can be used to modulate neuronal oscillations in vitro and in vivo. |
| first_indexed | 2024-12-19T22:35:45Z |
| format | Article |
| id | doaj.art-54a1aa74b23b437eb610dea17c681436 |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-12-19T22:35:45Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-54a1aa74b23b437eb610dea17c6814362022-12-21T20:03:12ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2021-09-011510.3389/fnins.2021.718311718311A Closed-Loop Optogenetic PlatformDimitrios Firfilionis0Frances Hutchings1Reza Tamadoni2Darren Walsh3Mark Turnbull4Enrique Escobedo-Cousin5Richard G. Bailey6Johannes Gausden7Aaliyah Patel8Dorian Haci9Yan Liu10Yan Liu11Fiona E. N. LeBeau12Andrew Trevelyan13Timothy G. Constandinou14Timothy G. Constandinou15Anthony O'Neill16Marcus Kaiser17Marcus Kaiser18Marcus Kaiser19Patrick Degenaar20Andrew Jackson21Neuroprosthesis Lab, School of Engineering, Newcastle University, Newcastle upon Tyne, United KingdomDigital Institute, Newcastle University, Newcastle upon Tyne, United KingdomNeuroprosthesis Lab, School of Engineering, Newcastle University, Newcastle upon Tyne, United KingdomBiosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, United KingdomBiosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, United KingdomEmerging Technologies and Materials Group, School of Engineering, Newcastle University, Newcastle upon Tyne, United KingdomEmerging Technologies and Materials Group, School of Engineering, Newcastle University, Newcastle upon Tyne, United KingdomEmerging Technologies and Materials Group, School of Engineering, Newcastle University, Newcastle upon Tyne, United KingdomEmerging Technologies and Materials Group, School of Engineering, Newcastle University, Newcastle upon Tyne, United KingdomDepartment of Electrical and Electronic Engineering, Imperial College London, London, United KingdomDepartment of Electrical and Electronic Engineering, Imperial College London, London, United KingdomDepartment of Micro-Nano Electronics, Shanghai Jiaotong University, Shanghai, ChinaBiosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, United KingdomBiosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, United KingdomDepartment of Electrical and Electronic Engineering, Imperial College London, London, United KingdomCare Research and Technology Centre, UK Dementia Research Institute, London, United KingdomEmerging Technologies and Materials Group, School of Engineering, Newcastle University, Newcastle upon Tyne, United KingdomSchool of Computing, Newcastle University, Newcastle upon Tyne, United KingdomSchool of Medicine, University of Nottingham, Nottingham, United Kingdom0Rui Jin Hospital, Shanghai Jiao Tong University, Shanghai, ChinaNeuroprosthesis Lab, School of Engineering, Newcastle University, Newcastle upon Tyne, United KingdomBiosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, United KingdomNeuromodulation is an established treatment for numerous neurological conditions, but to expand the therapeutic scope there is a need to improve the spatial, temporal and cell-type specificity of stimulation. Optogenetics is a promising area of current research, enabling optical stimulation of genetically-defined cell types without interfering with concurrent electrical recording for closed-loop control of neural activity. We are developing an open-source system to provide a platform for closed-loop optogenetic neuromodulation, incorporating custom integrated circuitry for recording and stimulation, real-time closed-loop algorithms running on a microcontroller and experimental control via a PC interface. We include commercial components to validate performance, with the ultimate aim of translating this approach to humans. In the meantime our system is flexible and expandable for use in a variety of preclinical neuroscientific applications. The platform consists of a Controlling Abnormal Network Dynamics using Optogenetics (CANDO) Control System (CS) that interfaces with up to four CANDO headstages responsible for electrical recording and optical stimulation through custom CANDO LED optrodes. Control of the hardware, inbuilt algorithms and data acquisition is enabled via the CANDO GUI (Graphical User Interface). Here we describe the design and implementation of this system, and demonstrate how it can be used to modulate neuronal oscillations in vitro and in vivo.https://www.frontiersin.org/articles/10.3389/fnins.2021.718311/fulloptogeneticsclosed-loopneuromodulationelectrophysiologyopen-source |
| spellingShingle | Dimitrios Firfilionis Frances Hutchings Reza Tamadoni Darren Walsh Mark Turnbull Enrique Escobedo-Cousin Richard G. Bailey Johannes Gausden Aaliyah Patel Dorian Haci Yan Liu Yan Liu Fiona E. N. LeBeau Andrew Trevelyan Timothy G. Constandinou Timothy G. Constandinou Anthony O'Neill Marcus Kaiser Marcus Kaiser Marcus Kaiser Patrick Degenaar Andrew Jackson A Closed-Loop Optogenetic Platform Frontiers in Neuroscience optogenetics closed-loop neuromodulation electrophysiology open-source |
| title | A Closed-Loop Optogenetic Platform |
| title_full | A Closed-Loop Optogenetic Platform |
| title_fullStr | A Closed-Loop Optogenetic Platform |
| title_full_unstemmed | A Closed-Loop Optogenetic Platform |
| title_short | A Closed-Loop Optogenetic Platform |
| title_sort | closed loop optogenetic platform |
| topic | optogenetics closed-loop neuromodulation electrophysiology open-source |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2021.718311/full |
| work_keys_str_mv | AT dimitriosfirfilionis aclosedloopoptogeneticplatform AT franceshutchings aclosedloopoptogeneticplatform AT rezatamadoni aclosedloopoptogeneticplatform AT darrenwalsh aclosedloopoptogeneticplatform AT markturnbull aclosedloopoptogeneticplatform AT enriqueescobedocousin aclosedloopoptogeneticplatform AT richardgbailey aclosedloopoptogeneticplatform AT johannesgausden aclosedloopoptogeneticplatform AT aaliyahpatel aclosedloopoptogeneticplatform AT dorianhaci aclosedloopoptogeneticplatform AT yanliu aclosedloopoptogeneticplatform AT yanliu aclosedloopoptogeneticplatform AT fionaenlebeau aclosedloopoptogeneticplatform AT andrewtrevelyan aclosedloopoptogeneticplatform AT timothygconstandinou aclosedloopoptogeneticplatform AT timothygconstandinou aclosedloopoptogeneticplatform AT anthonyoneill aclosedloopoptogeneticplatform AT marcuskaiser aclosedloopoptogeneticplatform AT marcuskaiser aclosedloopoptogeneticplatform AT marcuskaiser aclosedloopoptogeneticplatform AT patrickdegenaar aclosedloopoptogeneticplatform AT andrewjackson aclosedloopoptogeneticplatform AT dimitriosfirfilionis closedloopoptogeneticplatform AT franceshutchings closedloopoptogeneticplatform AT rezatamadoni closedloopoptogeneticplatform AT darrenwalsh closedloopoptogeneticplatform AT markturnbull closedloopoptogeneticplatform AT enriqueescobedocousin closedloopoptogeneticplatform AT richardgbailey closedloopoptogeneticplatform AT johannesgausden closedloopoptogeneticplatform AT aaliyahpatel closedloopoptogeneticplatform AT dorianhaci closedloopoptogeneticplatform AT yanliu closedloopoptogeneticplatform AT yanliu closedloopoptogeneticplatform AT fionaenlebeau closedloopoptogeneticplatform AT andrewtrevelyan closedloopoptogeneticplatform AT timothygconstandinou closedloopoptogeneticplatform AT timothygconstandinou closedloopoptogeneticplatform AT anthonyoneill closedloopoptogeneticplatform AT marcuskaiser closedloopoptogeneticplatform AT marcuskaiser closedloopoptogeneticplatform AT marcuskaiser closedloopoptogeneticplatform AT patrickdegenaar closedloopoptogeneticplatform AT andrewjackson closedloopoptogeneticplatform |