Periostin Contributes to the Pathogenesis of Atopic Dermatitis by Inducing TSLP Production from Keratinocytes
Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease where Th2-type immune responses are dominant. Keratinocytes persistently secrete proinflammatory cytokines and chemokines, amplifying Th2-type responses in AD. We have recently reported that periostin, an extracellular matrix...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
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Elsevier
2012-01-01
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| Series: | Allergology International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1323893015302574 |
| _version_ | 1818020699771502592 |
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| author | Hiroshi Shiraishi Miho Masuoka Shoichiro Ohta Shoichi Suzuki Kazuhiko Arima Kazuto Taniguchi Shigehisa Aoki Shuji Toda Tomohiro Yoshimoto Naoki Inagaki Simon J Conway Yutaka Narisawa Kenji Izuhara |
| author_facet | Hiroshi Shiraishi Miho Masuoka Shoichiro Ohta Shoichi Suzuki Kazuhiko Arima Kazuto Taniguchi Shigehisa Aoki Shuji Toda Tomohiro Yoshimoto Naoki Inagaki Simon J Conway Yutaka Narisawa Kenji Izuhara |
| author_sort | Hiroshi Shiraishi |
| collection | DOAJ |
| description | Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease where Th2-type immune responses are dominant. Keratinocytes persistently secrete proinflammatory cytokines and chemokines, amplifying Th2-type responses in AD. We have recently reported that periostin, an extracellular matrix protein induced by Th2 cytokines, plays a critical role in AD. In the present study, we have further investigated the characteristics of our allergen-induced AD model mice and the role of periostin in the pathogenesis of AD.
Methods: The ears of C57BL/6 mice, BALB/c mice, and Rag-2−/− γc−/− mice (BALB/c background) were epicutaneously sensitized repeatedly with HDM. Mice were analyzed after the final sensitization. To examine the direct role of periostin, we reconstituted skin in vitro by coculture of keratinocytes with wild-type or periostin-deficient fibroblasts.
Results: Epicutaneous sensitization with HDM induced AD-like phenotypes and accumulation of periostin in dermis in C57BL/6 mice but not in Rag-2−/− γc−/− mice. In vitro organotypic coculture systems revealed that periostin promoted survival and proliferation of keratinocytes and directly induced production of thymic stromal lymphopoietin (TSLP).
Conclusions: Our results suggest that periostin exacerbates the pathogenesis of AD through TSLP production from keratinocytes. |
| first_indexed | 2024-04-14T08:08:59Z |
| format | Article |
| id | doaj.art-54a307a5f6954527a6ce92516717a7ba |
| institution | Directory Open Access Journal |
| issn | 1323-8930 |
| language | English |
| last_indexed | 2024-04-14T08:08:59Z |
| publishDate | 2012-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Allergology International |
| spelling | doaj.art-54a307a5f6954527a6ce92516717a7ba2022-12-22T02:04:38ZengElsevierAllergology International1323-89302012-01-0161456357210.2332/allergolint.10-OA-0297Periostin Contributes to the Pathogenesis of Atopic Dermatitis by Inducing TSLP Production from KeratinocytesHiroshi Shiraishi0Miho Masuoka1Shoichiro Ohta2Shoichi Suzuki3Kazuhiko Arima4Kazuto Taniguchi5Shigehisa Aoki6Shuji Toda7Tomohiro Yoshimoto8Naoki Inagaki9Simon J Conway10Yutaka Narisawa11Kenji Izuhara12Division of Medical Biochemistry, Department of Biomolecular Sciences, JapanDivision of Medical Biochemistry, Department of Biomolecular Sciences, JapanDepartment of Laboratory Medicine, JapanDivision of Medical Biochemistry, Department of Biomolecular Sciences, JapanDivision of Medical Biochemistry, Department of Biomolecular Sciences, JapanDivision of Medical Biochemistry, Department of Biomolecular Sciences, JapanDepartment of Pathology & Biodefence, JapanDepartment of Pathology & Biodefence, JapanLaboratory of Allergic Diseases, Institute for Advanced Medical Sciences, Hyogo College of Medicine, Hyogo, JapanDepartment of Pharmacology, Gifu Pharmaceutical University, Gifu, JapanDevelopmental Biology and Neonatal Medicine Program, HB Wells Center for Pediatric Research; Indiana University School of Medicine, Indianapolis, IN, USA.Department of Dermatology, Saga Medical School, Saga, JapanDivision of Medical Biochemistry, Department of Biomolecular Sciences, JapanBackground: Atopic dermatitis (AD) is a chronic inflammatory skin disease where Th2-type immune responses are dominant. Keratinocytes persistently secrete proinflammatory cytokines and chemokines, amplifying Th2-type responses in AD. We have recently reported that periostin, an extracellular matrix protein induced by Th2 cytokines, plays a critical role in AD. In the present study, we have further investigated the characteristics of our allergen-induced AD model mice and the role of periostin in the pathogenesis of AD. Methods: The ears of C57BL/6 mice, BALB/c mice, and Rag-2−/− γc−/− mice (BALB/c background) were epicutaneously sensitized repeatedly with HDM. Mice were analyzed after the final sensitization. To examine the direct role of periostin, we reconstituted skin in vitro by coculture of keratinocytes with wild-type or periostin-deficient fibroblasts. Results: Epicutaneous sensitization with HDM induced AD-like phenotypes and accumulation of periostin in dermis in C57BL/6 mice but not in Rag-2−/− γc−/− mice. In vitro organotypic coculture systems revealed that periostin promoted survival and proliferation of keratinocytes and directly induced production of thymic stromal lymphopoietin (TSLP). Conclusions: Our results suggest that periostin exacerbates the pathogenesis of AD through TSLP production from keratinocytes.http://www.sciencedirect.com/science/article/pii/S1323893015302574atopic dermatitishouse dust miteperiostinTh2 cellsTSLP |
| spellingShingle | Hiroshi Shiraishi Miho Masuoka Shoichiro Ohta Shoichi Suzuki Kazuhiko Arima Kazuto Taniguchi Shigehisa Aoki Shuji Toda Tomohiro Yoshimoto Naoki Inagaki Simon J Conway Yutaka Narisawa Kenji Izuhara Periostin Contributes to the Pathogenesis of Atopic Dermatitis by Inducing TSLP Production from Keratinocytes Allergology International atopic dermatitis house dust mite periostin Th2 cells TSLP |
| title | Periostin Contributes to the Pathogenesis of Atopic Dermatitis by Inducing TSLP Production from Keratinocytes |
| title_full | Periostin Contributes to the Pathogenesis of Atopic Dermatitis by Inducing TSLP Production from Keratinocytes |
| title_fullStr | Periostin Contributes to the Pathogenesis of Atopic Dermatitis by Inducing TSLP Production from Keratinocytes |
| title_full_unstemmed | Periostin Contributes to the Pathogenesis of Atopic Dermatitis by Inducing TSLP Production from Keratinocytes |
| title_short | Periostin Contributes to the Pathogenesis of Atopic Dermatitis by Inducing TSLP Production from Keratinocytes |
| title_sort | periostin contributes to the pathogenesis of atopic dermatitis by inducing tslp production from keratinocytes |
| topic | atopic dermatitis house dust mite periostin Th2 cells TSLP |
| url | http://www.sciencedirect.com/science/article/pii/S1323893015302574 |
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