Proposed protective mechanism of the pancreas in the rat
<p>Abstract</p> <p>Background</p> <p>Heparan sulphate is known to have various functions in the animal body, including surveillance of tissue integrity. Administered intraperitoneally, it induces a systemic inflammatory response syndrome and when given locally in the pa...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-05-01
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| Series: | Journal of Inflammation |
| Online Access: | http://www.journal-inflammation.com/content/7/1/24 |
| _version_ | 1811246111629770752 |
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| author | Malmström Anders Said Katarzyna Akbarshahi Hamid Axelsson Jakob BF Andersson Roland |
| author_facet | Malmström Anders Said Katarzyna Akbarshahi Hamid Axelsson Jakob BF Andersson Roland |
| author_sort | Malmström Anders |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Heparan sulphate is known to have various functions in the animal body, including surveillance of tissue integrity. Administered intraperitoneally, it induces a systemic inflammatory response syndrome and when given locally in the pancreas it initiates a protective inflammatory response. The aim of the present study was to investigate the underlying mechanisms behind cell recruitment following intra-ductal infusion of heparan sulphate.</p> <p>Methods</p> <p>Rats were subjected to intraductal-infusion of heparan sulphate, lipopolysaccharide and phosphate buffered saline into the pancreas. Pancreatic tissue was harvested 1, 3, 6, 9 or 48 hours after infusion and stained immunohistochemically for myeloperoxidase, ED-1, CINC-1 and MCP-1, as well as using eosin hematoxylin staining. Furthermore, MPO activity and MCP-1 and CINC-1 concentrations of tissue homogenates were measured. All differences were analyzed statistically using the Mann-Whitney U-test.</p> <p>Results</p> <p>During HS infusion, a rapid influx of macrophages/monocytes, as visualized as ED-1 positive cells, was seen reaching a maximum at 6 hours. After 48 hours, the same levels of ED-1 positive cells were noted in the pancreatic tissue, but with different location and morphology. Increased neutrophil numbers of heparan sulphate treated animals compared to control could be detected only 9 hours after infusion. The number of neutrophils was lower than the number of ED-1 positive cells. On the contrary, LPS infusion caused increased neutrophil numbers to a larger extent than heparan sulphate. Furthermore, this accumulation of neutrophils preceded the infiltration of ED-1 positive cells. Chemokine expression correlates very well to the cell infiltrate. MCP-1 was evident in the ductal cells of both groups early on. MCP-1 preceded monocyte infiltration in both groups, while the CINC-1 increase was only noticeable in the LPS group.</p> <p>Conclusions</p> <p>Our data suggest that heparan and LPS both induce host defense reactions, though by using different mechanisms of cell-recruitment. This implies that the etiology of pancreatic inflammation may influence how the subsequent events will develop.</p> |
| first_indexed | 2024-04-12T14:48:55Z |
| format | Article |
| id | doaj.art-54a3157680f74684823280b4b20679d7 |
| institution | Directory Open Access Journal |
| issn | 1476-9255 |
| language | English |
| last_indexed | 2024-04-12T14:48:55Z |
| publishDate | 2010-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Inflammation |
| spelling | doaj.art-54a3157680f74684823280b4b20679d72022-12-22T03:28:33ZengBMCJournal of Inflammation1476-92552010-05-01712410.1186/1476-9255-7-24Proposed protective mechanism of the pancreas in the ratMalmström AndersSaid KatarzynaAkbarshahi HamidAxelsson Jakob BFAndersson Roland<p>Abstract</p> <p>Background</p> <p>Heparan sulphate is known to have various functions in the animal body, including surveillance of tissue integrity. Administered intraperitoneally, it induces a systemic inflammatory response syndrome and when given locally in the pancreas it initiates a protective inflammatory response. The aim of the present study was to investigate the underlying mechanisms behind cell recruitment following intra-ductal infusion of heparan sulphate.</p> <p>Methods</p> <p>Rats were subjected to intraductal-infusion of heparan sulphate, lipopolysaccharide and phosphate buffered saline into the pancreas. Pancreatic tissue was harvested 1, 3, 6, 9 or 48 hours after infusion and stained immunohistochemically for myeloperoxidase, ED-1, CINC-1 and MCP-1, as well as using eosin hematoxylin staining. Furthermore, MPO activity and MCP-1 and CINC-1 concentrations of tissue homogenates were measured. All differences were analyzed statistically using the Mann-Whitney U-test.</p> <p>Results</p> <p>During HS infusion, a rapid influx of macrophages/monocytes, as visualized as ED-1 positive cells, was seen reaching a maximum at 6 hours. After 48 hours, the same levels of ED-1 positive cells were noted in the pancreatic tissue, but with different location and morphology. Increased neutrophil numbers of heparan sulphate treated animals compared to control could be detected only 9 hours after infusion. The number of neutrophils was lower than the number of ED-1 positive cells. On the contrary, LPS infusion caused increased neutrophil numbers to a larger extent than heparan sulphate. Furthermore, this accumulation of neutrophils preceded the infiltration of ED-1 positive cells. Chemokine expression correlates very well to the cell infiltrate. MCP-1 was evident in the ductal cells of both groups early on. MCP-1 preceded monocyte infiltration in both groups, while the CINC-1 increase was only noticeable in the LPS group.</p> <p>Conclusions</p> <p>Our data suggest that heparan and LPS both induce host defense reactions, though by using different mechanisms of cell-recruitment. This implies that the etiology of pancreatic inflammation may influence how the subsequent events will develop.</p>http://www.journal-inflammation.com/content/7/1/24 |
| spellingShingle | Malmström Anders Said Katarzyna Akbarshahi Hamid Axelsson Jakob BF Andersson Roland Proposed protective mechanism of the pancreas in the rat Journal of Inflammation |
| title | Proposed protective mechanism of the pancreas in the rat |
| title_full | Proposed protective mechanism of the pancreas in the rat |
| title_fullStr | Proposed protective mechanism of the pancreas in the rat |
| title_full_unstemmed | Proposed protective mechanism of the pancreas in the rat |
| title_short | Proposed protective mechanism of the pancreas in the rat |
| title_sort | proposed protective mechanism of the pancreas in the rat |
| url | http://www.journal-inflammation.com/content/7/1/24 |
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