Surface quality and endothelial cell viability after femtosecond laser-assisted donor lenticule preparation for endothelial keratoplasty - An in-vitro study
Purpose: To compare surface quality and endothelial cell viability of descemet stripping automated endothelial keratoplasty (DSAEK) donor lenticules prepared with femtosecond laser (FSL) or microkeratome (MK). Methods: Experimental ex-vivo evaluation of 15 DSAEK donor lenticules prepared from optica...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2020-01-01
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| Series: | Indian Journal of Ophthalmology |
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| Online Access: | http://www.ijo.in/article.asp?issn=0301-4738;year=2020;volume=68;issue=11;spage=2404;epage=2407;aulast=Titiyal |
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| author | Jeewan S Titiyal M J Aravind Manpreet Kaur Tapas C Nag Namrata Sharma Tushar Agarwal Rajesh Sinha |
| author_facet | Jeewan S Titiyal M J Aravind Manpreet Kaur Tapas C Nag Namrata Sharma Tushar Agarwal Rajesh Sinha |
| author_sort | Jeewan S Titiyal |
| collection | DOAJ |
| description | Purpose: To compare surface quality and endothelial cell viability of descemet stripping automated endothelial keratoplasty (DSAEK) donor lenticules prepared with femtosecond laser (FSL) or microkeratome (MK). Methods: Experimental ex-vivo evaluation of 15 DSAEK donor lenticules prepared from optical quality donor corneas using 200 KHz FSL (9 eyes) or MK (6 eyes). Surface quality and smoothness of the cut were assessed using atomic force microscopy and endothelial cell viability was assessed using transmission electron microscopy. Results: Mean lenticule thickness was 121.89 ± 17.13 μm in FSL group and 112.67 ± 5.89 μm in MK group (P = 0.33). Average roughness of stromal surface (RMSavg) [FSL- 30.51 ± 4.55 nm, MK-22.37 ± 1.83 nm; P = 0.02] and root mean square roughness (RMSrough) [FSL-31.39 ± 5.75 nm, MK-23.08 ± 0.40 nm; P = 0.012] was significantly more in FSL group. Increased granular and linear irregularities were observed in the FSL group. Endothelial cell disruption was more in FSL group (FSL- 29.49 ± 6.91% MK-13.28 ± 3.62%; P < 0.001) with decreased mean nucleus length (FSL-5.56 ± 0.17 μm, MK-7.52 ± 0.65 μm; P < 0.001). Conclusion: Automated MKs are still the standard of care for donor lenticule preparation and MK-assisted donor lenticules have smoother surface with less endothelial cell disruption than FSL. Further research is mandatory before FSL platforms can be considered a viable alternative to the MK. |
| first_indexed | 2024-12-12T00:25:12Z |
| format | Article |
| id | doaj.art-54a632c14407465591ea123c22ab84a9 |
| institution | Directory Open Access Journal |
| issn | 0301-4738 1998-3689 |
| language | English |
| last_indexed | 2024-12-12T00:25:12Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Ophthalmology |
| spelling | doaj.art-54a632c14407465591ea123c22ab84a92022-12-22T00:44:38ZengWolters Kluwer Medknow PublicationsIndian Journal of Ophthalmology0301-47381998-36892020-01-0168112404240710.4103/ijo.IJO_99_20Surface quality and endothelial cell viability after femtosecond laser-assisted donor lenticule preparation for endothelial keratoplasty - An in-vitro studyJeewan S TitiyalM J AravindManpreet KaurTapas C NagNamrata SharmaTushar AgarwalRajesh SinhaPurpose: To compare surface quality and endothelial cell viability of descemet stripping automated endothelial keratoplasty (DSAEK) donor lenticules prepared with femtosecond laser (FSL) or microkeratome (MK). Methods: Experimental ex-vivo evaluation of 15 DSAEK donor lenticules prepared from optical quality donor corneas using 200 KHz FSL (9 eyes) or MK (6 eyes). Surface quality and smoothness of the cut were assessed using atomic force microscopy and endothelial cell viability was assessed using transmission electron microscopy. Results: Mean lenticule thickness was 121.89 ± 17.13 μm in FSL group and 112.67 ± 5.89 μm in MK group (P = 0.33). Average roughness of stromal surface (RMSavg) [FSL- 30.51 ± 4.55 nm, MK-22.37 ± 1.83 nm; P = 0.02] and root mean square roughness (RMSrough) [FSL-31.39 ± 5.75 nm, MK-23.08 ± 0.40 nm; P = 0.012] was significantly more in FSL group. Increased granular and linear irregularities were observed in the FSL group. Endothelial cell disruption was more in FSL group (FSL- 29.49 ± 6.91% MK-13.28 ± 3.62%; P < 0.001) with decreased mean nucleus length (FSL-5.56 ± 0.17 μm, MK-7.52 ± 0.65 μm; P < 0.001). Conclusion: Automated MKs are still the standard of care for donor lenticule preparation and MK-assisted donor lenticules have smoother surface with less endothelial cell disruption than FSL. Further research is mandatory before FSL platforms can be considered a viable alternative to the MK.http://www.ijo.in/article.asp?issn=0301-4738;year=2020;volume=68;issue=11;spage=2404;epage=2407;aulast=Titiyaldescemet stripping automated endothelial keratoplastyfemtosecond laser-assisted endothelial keratoplastyfemtosecond lasersmicrokeratome assisted endothelial keratoplasty |
| spellingShingle | Jeewan S Titiyal M J Aravind Manpreet Kaur Tapas C Nag Namrata Sharma Tushar Agarwal Rajesh Sinha Surface quality and endothelial cell viability after femtosecond laser-assisted donor lenticule preparation for endothelial keratoplasty - An in-vitro study Indian Journal of Ophthalmology descemet stripping automated endothelial keratoplasty femtosecond laser-assisted endothelial keratoplasty femtosecond lasers microkeratome assisted endothelial keratoplasty |
| title | Surface quality and endothelial cell viability after femtosecond laser-assisted donor lenticule preparation for endothelial keratoplasty - An in-vitro study |
| title_full | Surface quality and endothelial cell viability after femtosecond laser-assisted donor lenticule preparation for endothelial keratoplasty - An in-vitro study |
| title_fullStr | Surface quality and endothelial cell viability after femtosecond laser-assisted donor lenticule preparation for endothelial keratoplasty - An in-vitro study |
| title_full_unstemmed | Surface quality and endothelial cell viability after femtosecond laser-assisted donor lenticule preparation for endothelial keratoplasty - An in-vitro study |
| title_short | Surface quality and endothelial cell viability after femtosecond laser-assisted donor lenticule preparation for endothelial keratoplasty - An in-vitro study |
| title_sort | surface quality and endothelial cell viability after femtosecond laser assisted donor lenticule preparation for endothelial keratoplasty an in vitro study |
| topic | descemet stripping automated endothelial keratoplasty femtosecond laser-assisted endothelial keratoplasty femtosecond lasers microkeratome assisted endothelial keratoplasty |
| url | http://www.ijo.in/article.asp?issn=0301-4738;year=2020;volume=68;issue=11;spage=2404;epage=2407;aulast=Titiyal |
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