Proanthocyanidins Alleviates AflatoxinB1-Induced Oxidative Stress and Apoptosis through Mitochondrial Pathway in the Bursa of Fabricius of Broilers
Aflatoxin B1 (AFB1) is a serious threat to the poultry industry. Proanthocyanidins (PCs) demonstrates a broad range of biological, pharmacological, therapeutic, and chemoprotective properties. The aim of this study was to investigate the ameliorative effects of PCs against AFB1-induced histopatholog...
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MDPI AG
2019-03-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/11/3/157 |
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| author | Shahid Ali Rajput Cong Zhang Yue Feng Xiao Tian Wei Mahmoud Mohamed Khalil Imran Rashid Rajput Dost Muhammad Baloch Aftab Shaukat Nasir Rajput Hammad Qamar Mubashar Hassan Desheng Qi |
| author_facet | Shahid Ali Rajput Cong Zhang Yue Feng Xiao Tian Wei Mahmoud Mohamed Khalil Imran Rashid Rajput Dost Muhammad Baloch Aftab Shaukat Nasir Rajput Hammad Qamar Mubashar Hassan Desheng Qi |
| author_sort | Shahid Ali Rajput |
| collection | DOAJ |
| description | Aflatoxin B1 (AFB1) is a serious threat to the poultry industry. Proanthocyanidins (PCs) demonstrates a broad range of biological, pharmacological, therapeutic, and chemoprotective properties. The aim of this study was to investigate the ameliorative effects of PCs against AFB1-induced histopathology, oxidative stress, and apoptosis via the mitochondrial pathway in the bursa of Fabricius (BF) of broilers. One hundred forty-four one-day old Cobb chicks were randomly assigned into four treatment groups of six replicates (6 birds each replicate) for 28 days. Groups were fed on the following four diets; (1) Basal diet without addition of PCs or AFB1 (Control); (2) basal diet supplemented with 1 mg/kg AFB1 from contaminated corn (AFB1); (3) basal diet supplemented with 250 mg/kg PCs (PCs); and (4) basal diet supplemented with 1 mg/kg AFB1 + 250 mg/kg PCs (AFB1+ PCs). The present study results showed that antioxidant enzymes activities of total superoxide dismutase (T-SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and glutathione S-transferase (GST) in AFB1 treated group were (p < 0.05) decreased, whereas malondialdehyde (MDA) contents were significantly increased in comparison with the control group. Furthermore, we found that dietary PCs treatment ameliorated AFB1-induced oxidative stress in the BF through inhibiting the accumulation of MDA content and enhancing the antioxidant enzymes activities (T-SOD, CAT, GSH-Px, and GST). Similarly, PCs markedly enhanced messenger RNA (mRNA) expression of antioxidant genes (SOD, CAT, GPx1, and GST) in comparison with AFB1 group. Moreover, histological results showed that PCs alleviated AFB1-induced apoptotic cells in the BF of broilers. In addition, both mRNA and protein expression results manifested that mitochondrial-apoptosis-associated genes (Bax, caspase-9, caspase-3, and p53 and cytochrome c) showed up-regulation, while (Bcl-2) showed down-regulation in AFB1 fed group. The supplementation of PCs to AFB1 diet significantly reversed the mRNA and protein expression of these apoptosis-associated genes, as compared to the AFB1 group. Our results demonstrated that PCs ameliorated AFB1-induced oxidative stress by modulating the antioxidant defense system and apoptosis in the BF through mitochondrial pathway in broilers. |
| first_indexed | 2024-04-11T13:27:42Z |
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| last_indexed | 2024-04-11T13:27:42Z |
| publishDate | 2019-03-01 |
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| spelling | doaj.art-54a641a3be954ca1a0582e5a954ef97f2022-12-22T04:21:59ZengMDPI AGToxins2072-66512019-03-0111315710.3390/toxins11030157toxins11030157Proanthocyanidins Alleviates AflatoxinB1-Induced Oxidative Stress and Apoptosis through Mitochondrial Pathway in the Bursa of Fabricius of BroilersShahid Ali Rajput0Cong Zhang1Yue Feng2Xiao Tian Wei3Mahmoud Mohamed Khalil4Imran Rashid Rajput5Dost Muhammad Baloch6Aftab Shaukat7Nasir Rajput8Hammad Qamar9Mubashar Hassan10Desheng Qi11Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaAnimal Production Department, Faculty of Agriculture, Benha University, 13736 Banha, EgyptFaculty of Veterinary and Animal Science, Department of Biotechnology, Lasbela Univesity of Agriculture Water and Marine Science, 89250 Uthal, Balochistan, PakistanFaculty of Veterinary and Animal Science, Department of Biotechnology, Lasbela Univesity of Agriculture Water and Marine Science, 89250 Uthal, Balochistan, PakistanCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Poultry Husbandry, Faculty of Animal Husbandry and Veterinary Science, Sindh Agriculture University, 70060 Tandojam, PakistanResearch Center of Animal Nutrition and Metabolic Diseases, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaAflatoxin B1 (AFB1) is a serious threat to the poultry industry. Proanthocyanidins (PCs) demonstrates a broad range of biological, pharmacological, therapeutic, and chemoprotective properties. The aim of this study was to investigate the ameliorative effects of PCs against AFB1-induced histopathology, oxidative stress, and apoptosis via the mitochondrial pathway in the bursa of Fabricius (BF) of broilers. One hundred forty-four one-day old Cobb chicks were randomly assigned into four treatment groups of six replicates (6 birds each replicate) for 28 days. Groups were fed on the following four diets; (1) Basal diet without addition of PCs or AFB1 (Control); (2) basal diet supplemented with 1 mg/kg AFB1 from contaminated corn (AFB1); (3) basal diet supplemented with 250 mg/kg PCs (PCs); and (4) basal diet supplemented with 1 mg/kg AFB1 + 250 mg/kg PCs (AFB1+ PCs). The present study results showed that antioxidant enzymes activities of total superoxide dismutase (T-SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and glutathione S-transferase (GST) in AFB1 treated group were (p < 0.05) decreased, whereas malondialdehyde (MDA) contents were significantly increased in comparison with the control group. Furthermore, we found that dietary PCs treatment ameliorated AFB1-induced oxidative stress in the BF through inhibiting the accumulation of MDA content and enhancing the antioxidant enzymes activities (T-SOD, CAT, GSH-Px, and GST). Similarly, PCs markedly enhanced messenger RNA (mRNA) expression of antioxidant genes (SOD, CAT, GPx1, and GST) in comparison with AFB1 group. Moreover, histological results showed that PCs alleviated AFB1-induced apoptotic cells in the BF of broilers. In addition, both mRNA and protein expression results manifested that mitochondrial-apoptosis-associated genes (Bax, caspase-9, caspase-3, and p53 and cytochrome c) showed up-regulation, while (Bcl-2) showed down-regulation in AFB1 fed group. The supplementation of PCs to AFB1 diet significantly reversed the mRNA and protein expression of these apoptosis-associated genes, as compared to the AFB1 group. Our results demonstrated that PCs ameliorated AFB1-induced oxidative stress by modulating the antioxidant defense system and apoptosis in the BF through mitochondrial pathway in broilers.http://www.mdpi.com/2072-6651/11/3/157aflatoxin B1proanthocyanidinsoxidative stressapoptosisbursa of Fabriciusbroiler |
| spellingShingle | Shahid Ali Rajput Cong Zhang Yue Feng Xiao Tian Wei Mahmoud Mohamed Khalil Imran Rashid Rajput Dost Muhammad Baloch Aftab Shaukat Nasir Rajput Hammad Qamar Mubashar Hassan Desheng Qi Proanthocyanidins Alleviates AflatoxinB1-Induced Oxidative Stress and Apoptosis through Mitochondrial Pathway in the Bursa of Fabricius of Broilers Toxins aflatoxin B1 proanthocyanidins oxidative stress apoptosis bursa of Fabricius broiler |
| title | Proanthocyanidins Alleviates AflatoxinB1-Induced Oxidative Stress and Apoptosis through Mitochondrial Pathway in the Bursa of Fabricius of Broilers |
| title_full | Proanthocyanidins Alleviates AflatoxinB1-Induced Oxidative Stress and Apoptosis through Mitochondrial Pathway in the Bursa of Fabricius of Broilers |
| title_fullStr | Proanthocyanidins Alleviates AflatoxinB1-Induced Oxidative Stress and Apoptosis through Mitochondrial Pathway in the Bursa of Fabricius of Broilers |
| title_full_unstemmed | Proanthocyanidins Alleviates AflatoxinB1-Induced Oxidative Stress and Apoptosis through Mitochondrial Pathway in the Bursa of Fabricius of Broilers |
| title_short | Proanthocyanidins Alleviates AflatoxinB1-Induced Oxidative Stress and Apoptosis through Mitochondrial Pathway in the Bursa of Fabricius of Broilers |
| title_sort | proanthocyanidins alleviates aflatoxinb1 induced oxidative stress and apoptosis through mitochondrial pathway in the bursa of fabricius of broilers |
| topic | aflatoxin B1 proanthocyanidins oxidative stress apoptosis bursa of Fabricius broiler |
| url | http://www.mdpi.com/2072-6651/11/3/157 |
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