A Monolayer System for the Efficient Generation of Motor Neuron Progenitors and Functional Motor Neurons from Human Pluripotent Stem Cells
Methods for the conversion of human induced pluripotent stem cells (hiPSCs) into motor neurons (MNs) have opened to the generation of patient-derived in vitro systems that can be exploited for MN disease modelling. However, the lack of simplified and consistent protocols and the fact that hiPSC-deri...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/10/5/1127 |
| _version_ | 1797535007563055104 |
|---|---|
| author | Alessandro Cutarelli Vladimir A. Martínez-Rojas Alice Tata Ingrid Battistella Daniela Rossi Daniele Arosio Carlo Musio Luciano Conti |
| author_facet | Alessandro Cutarelli Vladimir A. Martínez-Rojas Alice Tata Ingrid Battistella Daniela Rossi Daniele Arosio Carlo Musio Luciano Conti |
| author_sort | Alessandro Cutarelli |
| collection | DOAJ |
| description | Methods for the conversion of human induced pluripotent stem cells (hiPSCs) into motor neurons (MNs) have opened to the generation of patient-derived in vitro systems that can be exploited for MN disease modelling. However, the lack of simplified and consistent protocols and the fact that hiPSC-derived MNs are often functionally immature yet limit the opportunity to fully take advantage of this technology, especially in research aimed at revealing the disease phenotypes that are manifested in functionally mature cells. In this study, we present a robust, optimized monolayer procedure to rapidly convert hiPSCs into enriched populations of motor neuron progenitor cells (MNPCs) that can be further amplified to produce a large number of cells to cover many experimental needs. These MNPCs can be efficiently differentiated towards mature MNs exhibiting functional electrical and pharmacological neuronal properties. Finally, we report that MN cultures can be long-term maintained, thus offering the opportunity to study degenerative phenomena associated with pathologies involving MNs and their functional, networked activity. These results indicate that our optimized procedure enables the efficient and robust generation of large quantities of MNPCs and functional MNs, providing a valid tool for MNs disease modelling and for drug discovery applications. |
| first_indexed | 2024-03-10T11:38:29Z |
| format | Article |
| id | doaj.art-54a6ad3ff9fc47229432a8c822775005 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T11:38:29Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-54a6ad3ff9fc47229432a8c8227750052023-11-21T18:38:36ZengMDPI AGCells2073-44092021-05-01105112710.3390/cells10051127A Monolayer System for the Efficient Generation of Motor Neuron Progenitors and Functional Motor Neurons from Human Pluripotent Stem CellsAlessandro Cutarelli0Vladimir A. Martínez-Rojas1Alice Tata2Ingrid Battistella3Daniela Rossi4Daniele Arosio5Carlo Musio6Luciano Conti7Laboratory of Stem Cell Biology, Department of Cellular, Computational and Integrative Biology-CIBIO, University of Trento, 38123 Trento, ItalyInstitute of Biophysics (IBF), Trento Unit, National Research Council (CNR) & LabSSAH, Bruno Kessler Foundation (FBK), 38123 Trento, ItalyLaboratory of Stem Cell Biology, Department of Cellular, Computational and Integrative Biology-CIBIO, University of Trento, 38123 Trento, ItalyLaboratory of Stem Cell Biology, Department of Cellular, Computational and Integrative Biology-CIBIO, University of Trento, 38123 Trento, ItalyLaboratory for Research on Neurodegenerative Disorders, Istituti Clinici Scientifici Maugeri IRCCS, 27100 Pavia, ItalyInstitute of Biophysics (IBF), Trento Unit, National Research Council (CNR) & LabSSAH, Bruno Kessler Foundation (FBK), 38123 Trento, ItalyInstitute of Biophysics (IBF), Trento Unit, National Research Council (CNR) & LabSSAH, Bruno Kessler Foundation (FBK), 38123 Trento, ItalyLaboratory of Stem Cell Biology, Department of Cellular, Computational and Integrative Biology-CIBIO, University of Trento, 38123 Trento, ItalyMethods for the conversion of human induced pluripotent stem cells (hiPSCs) into motor neurons (MNs) have opened to the generation of patient-derived in vitro systems that can be exploited for MN disease modelling. However, the lack of simplified and consistent protocols and the fact that hiPSC-derived MNs are often functionally immature yet limit the opportunity to fully take advantage of this technology, especially in research aimed at revealing the disease phenotypes that are manifested in functionally mature cells. In this study, we present a robust, optimized monolayer procedure to rapidly convert hiPSCs into enriched populations of motor neuron progenitor cells (MNPCs) that can be further amplified to produce a large number of cells to cover many experimental needs. These MNPCs can be efficiently differentiated towards mature MNs exhibiting functional electrical and pharmacological neuronal properties. Finally, we report that MN cultures can be long-term maintained, thus offering the opportunity to study degenerative phenomena associated with pathologies involving MNs and their functional, networked activity. These results indicate that our optimized procedure enables the efficient and robust generation of large quantities of MNPCs and functional MNs, providing a valid tool for MNs disease modelling and for drug discovery applications.https://www.mdpi.com/2073-4409/10/5/1127induced pluripotent stem cellshiPSCspinal motor neuronsmotor neuron progenitorscellular modelsspinal muscular atrophy |
| spellingShingle | Alessandro Cutarelli Vladimir A. Martínez-Rojas Alice Tata Ingrid Battistella Daniela Rossi Daniele Arosio Carlo Musio Luciano Conti A Monolayer System for the Efficient Generation of Motor Neuron Progenitors and Functional Motor Neurons from Human Pluripotent Stem Cells Cells induced pluripotent stem cells hiPSC spinal motor neurons motor neuron progenitors cellular models spinal muscular atrophy |
| title | A Monolayer System for the Efficient Generation of Motor Neuron Progenitors and Functional Motor Neurons from Human Pluripotent Stem Cells |
| title_full | A Monolayer System for the Efficient Generation of Motor Neuron Progenitors and Functional Motor Neurons from Human Pluripotent Stem Cells |
| title_fullStr | A Monolayer System for the Efficient Generation of Motor Neuron Progenitors and Functional Motor Neurons from Human Pluripotent Stem Cells |
| title_full_unstemmed | A Monolayer System for the Efficient Generation of Motor Neuron Progenitors and Functional Motor Neurons from Human Pluripotent Stem Cells |
| title_short | A Monolayer System for the Efficient Generation of Motor Neuron Progenitors and Functional Motor Neurons from Human Pluripotent Stem Cells |
| title_sort | monolayer system for the efficient generation of motor neuron progenitors and functional motor neurons from human pluripotent stem cells |
| topic | induced pluripotent stem cells hiPSC spinal motor neurons motor neuron progenitors cellular models spinal muscular atrophy |
| url | https://www.mdpi.com/2073-4409/10/5/1127 |
| work_keys_str_mv | AT alessandrocutarelli amonolayersystemfortheefficientgenerationofmotorneuronprogenitorsandfunctionalmotorneuronsfromhumanpluripotentstemcells AT vladimiramartinezrojas amonolayersystemfortheefficientgenerationofmotorneuronprogenitorsandfunctionalmotorneuronsfromhumanpluripotentstemcells AT alicetata amonolayersystemfortheefficientgenerationofmotorneuronprogenitorsandfunctionalmotorneuronsfromhumanpluripotentstemcells AT ingridbattistella amonolayersystemfortheefficientgenerationofmotorneuronprogenitorsandfunctionalmotorneuronsfromhumanpluripotentstemcells AT danielarossi amonolayersystemfortheefficientgenerationofmotorneuronprogenitorsandfunctionalmotorneuronsfromhumanpluripotentstemcells AT danielearosio amonolayersystemfortheefficientgenerationofmotorneuronprogenitorsandfunctionalmotorneuronsfromhumanpluripotentstemcells AT carlomusio amonolayersystemfortheefficientgenerationofmotorneuronprogenitorsandfunctionalmotorneuronsfromhumanpluripotentstemcells AT lucianoconti amonolayersystemfortheefficientgenerationofmotorneuronprogenitorsandfunctionalmotorneuronsfromhumanpluripotentstemcells AT alessandrocutarelli monolayersystemfortheefficientgenerationofmotorneuronprogenitorsandfunctionalmotorneuronsfromhumanpluripotentstemcells AT vladimiramartinezrojas monolayersystemfortheefficientgenerationofmotorneuronprogenitorsandfunctionalmotorneuronsfromhumanpluripotentstemcells AT alicetata monolayersystemfortheefficientgenerationofmotorneuronprogenitorsandfunctionalmotorneuronsfromhumanpluripotentstemcells AT ingridbattistella monolayersystemfortheefficientgenerationofmotorneuronprogenitorsandfunctionalmotorneuronsfromhumanpluripotentstemcells AT danielarossi monolayersystemfortheefficientgenerationofmotorneuronprogenitorsandfunctionalmotorneuronsfromhumanpluripotentstemcells AT danielearosio monolayersystemfortheefficientgenerationofmotorneuronprogenitorsandfunctionalmotorneuronsfromhumanpluripotentstemcells AT carlomusio monolayersystemfortheefficientgenerationofmotorneuronprogenitorsandfunctionalmotorneuronsfromhumanpluripotentstemcells AT lucianoconti monolayersystemfortheefficientgenerationofmotorneuronprogenitorsandfunctionalmotorneuronsfromhumanpluripotentstemcells |