Phenotypic and Functional Characterizations of Mesenchymal Stem/Stromal Cells Isolated From Human Cranial Bone Marrow
Mesenchymal stem/stromal cells (MSCs) are adult stem cells that were originally isolated from bone marrow. In contrast to long bone-derived MSCs that have been extensively characterized, our knowledge regarding to MSCs isolated from flat bones (e.g., cranial bones) remain less clear. In this study,...
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| Format: | Article |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2022.909256/full |
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| author | Kaichuang Yang Kaichuang Yang Ruijie Lu Jianan Lu Shucai Fan Qiang Zhang Qiang Zhang Zijian Lou Yuyuan Ma Gang Lu Ruolang Pan Ruolang Pan Jianmin Zhang |
| author_facet | Kaichuang Yang Kaichuang Yang Ruijie Lu Jianan Lu Shucai Fan Qiang Zhang Qiang Zhang Zijian Lou Yuyuan Ma Gang Lu Ruolang Pan Ruolang Pan Jianmin Zhang |
| author_sort | Kaichuang Yang |
| collection | DOAJ |
| description | Mesenchymal stem/stromal cells (MSCs) are adult stem cells that were originally isolated from bone marrow. In contrast to long bone-derived MSCs that have been extensively characterized, our knowledge regarding to MSCs isolated from flat bones (e.g., cranial bones) remain less clear. In this study, MSCs were purified from human cranial bone marrow (CB-MSCs) and their transdifferentiation capacity and immunomodulatory functions were further characterized. Phenotypic analysis of CB-MSCs demonstrated high expression of CD73, CD90, and CD105 while negative for CD14, CD34, and HLA-DR. Further in vitro differentiation assay shown that CB-MSCs capable of differentiating into cell types of mesenchymal origin (i.e., adipocytes, osetoblasts, and chondrocytes) and collectively, these results indicated that cells isolated from cranial bone marrow in this study are bona fide MSCs according to the minimal criteria proposed by the International Society for Cellular Therapy. Following in vitro expansion, single colony-derived CB-MSCs (scCB-MSCs) were obtained and confocal microscopy analysis further revealed functional heterogeneity within primary CB-MSCs. Specifically, obtained scCB-MSCs exhibited GABA progenitor features, as determined by olig2 and nestin. As expect, scCB-MSCs were readily induced to differentiate into GABAergic neuron-like cells. Furthermore, immunomodulatory roles of scCB-MSCs were evaluated following co-culture with human peripheral blood lymphocytes and results shown that co-culturing with scCB-MSCs significantly suppressed lymphocyte proliferation and promoted differentiation of lymphocytes into regulatory T cells but not Th1/Th17 phenotype. Overall, our results indicated that CB-MSCs exhibited clonal heterogeneity with marked propensity to differentiate into neural-like cells and this might represent promising candidates for the treatment of neurodegenerative diseases. |
| first_indexed | 2024-04-13T21:19:25Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-04-13T21:19:25Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-54a85c68b02a4993ae550843116b4bf52022-12-22T02:29:34ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2022-06-011610.3389/fnins.2022.909256909256Phenotypic and Functional Characterizations of Mesenchymal Stem/Stromal Cells Isolated From Human Cranial Bone MarrowKaichuang Yang0Kaichuang Yang1Ruijie Lu2Jianan Lu3Shucai Fan4Qiang Zhang5Qiang Zhang6Zijian Lou7Yuyuan Ma8Gang Lu9Ruolang Pan10Ruolang Pan11Jianmin Zhang12Department of Neurosurgery, Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, ChinaCenter for Rehabilitation Medicine, Department of Neurosurgery, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, ChinaThe Second Clinical Medical College, Wenzhou Medical University, Wenzhou, ChinaDepartment of Neurosurgery, Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, ChinaCenter for Rehabilitation Medicine, Department of Neurosurgery, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, ChinaKey Laboratory of Cell-Based Drug and Applied Technology Development in Zhejiang Province, Hangzhou, ChinaInstitute for Cell-Based Drug Development of Zhejiang Province, S-Evans Biosciences, Hangzhou, ChinaThe Second Clinical Medical College, Wenzhou Medical University, Wenzhou, ChinaCenter for Rehabilitation Medicine, Department of Neurosurgery, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, ChinaCenter for Rehabilitation Medicine, Department of Neurosurgery, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, ChinaKey Laboratory of Cell-Based Drug and Applied Technology Development in Zhejiang Province, Hangzhou, ChinaInstitute for Cell-Based Drug Development of Zhejiang Province, S-Evans Biosciences, Hangzhou, ChinaDepartment of Neurosurgery, Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, ChinaMesenchymal stem/stromal cells (MSCs) are adult stem cells that were originally isolated from bone marrow. In contrast to long bone-derived MSCs that have been extensively characterized, our knowledge regarding to MSCs isolated from flat bones (e.g., cranial bones) remain less clear. In this study, MSCs were purified from human cranial bone marrow (CB-MSCs) and their transdifferentiation capacity and immunomodulatory functions were further characterized. Phenotypic analysis of CB-MSCs demonstrated high expression of CD73, CD90, and CD105 while negative for CD14, CD34, and HLA-DR. Further in vitro differentiation assay shown that CB-MSCs capable of differentiating into cell types of mesenchymal origin (i.e., adipocytes, osetoblasts, and chondrocytes) and collectively, these results indicated that cells isolated from cranial bone marrow in this study are bona fide MSCs according to the minimal criteria proposed by the International Society for Cellular Therapy. Following in vitro expansion, single colony-derived CB-MSCs (scCB-MSCs) were obtained and confocal microscopy analysis further revealed functional heterogeneity within primary CB-MSCs. Specifically, obtained scCB-MSCs exhibited GABA progenitor features, as determined by olig2 and nestin. As expect, scCB-MSCs were readily induced to differentiate into GABAergic neuron-like cells. Furthermore, immunomodulatory roles of scCB-MSCs were evaluated following co-culture with human peripheral blood lymphocytes and results shown that co-culturing with scCB-MSCs significantly suppressed lymphocyte proliferation and promoted differentiation of lymphocytes into regulatory T cells but not Th1/Th17 phenotype. Overall, our results indicated that CB-MSCs exhibited clonal heterogeneity with marked propensity to differentiate into neural-like cells and this might represent promising candidates for the treatment of neurodegenerative diseases.https://www.frontiersin.org/articles/10.3389/fnins.2022.909256/fullcranial bonesmesenchymal stem/stromal cellstransdifferentiationimmunomodulationneural-like cells |
| spellingShingle | Kaichuang Yang Kaichuang Yang Ruijie Lu Jianan Lu Shucai Fan Qiang Zhang Qiang Zhang Zijian Lou Yuyuan Ma Gang Lu Ruolang Pan Ruolang Pan Jianmin Zhang Phenotypic and Functional Characterizations of Mesenchymal Stem/Stromal Cells Isolated From Human Cranial Bone Marrow Frontiers in Neuroscience cranial bones mesenchymal stem/stromal cells transdifferentiation immunomodulation neural-like cells |
| title | Phenotypic and Functional Characterizations of Mesenchymal Stem/Stromal Cells Isolated From Human Cranial Bone Marrow |
| title_full | Phenotypic and Functional Characterizations of Mesenchymal Stem/Stromal Cells Isolated From Human Cranial Bone Marrow |
| title_fullStr | Phenotypic and Functional Characterizations of Mesenchymal Stem/Stromal Cells Isolated From Human Cranial Bone Marrow |
| title_full_unstemmed | Phenotypic and Functional Characterizations of Mesenchymal Stem/Stromal Cells Isolated From Human Cranial Bone Marrow |
| title_short | Phenotypic and Functional Characterizations of Mesenchymal Stem/Stromal Cells Isolated From Human Cranial Bone Marrow |
| title_sort | phenotypic and functional characterizations of mesenchymal stem stromal cells isolated from human cranial bone marrow |
| topic | cranial bones mesenchymal stem/stromal cells transdifferentiation immunomodulation neural-like cells |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2022.909256/full |
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