Family and case–control genetic study of MSX1 polymorphisms in peg-shaped teeth Jordanian population
Abstract Background This study aimed to investigate the genetic association of specific Single Nucleotide Polymorphisms (SNPs) within the muscle segment homeobox gene 1 (MSX1) with susceptibility to the peg-shaped teeth in 36 Jordanian Arab families and case–control samples in the Jordanian Arab pop...
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| Format: | Article |
| Language: | English |
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BMC
2022-01-01
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| Series: | BMC Oral Health |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12903-022-02051-2 |
| _version_ | 1818954689966768128 |
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| author | Rami Alkhatib Razan Hawamdeh Laith Al-Eitan Nour Abdo Fadi Obeidat Mohamed Al-Bataineh Hatem Aman |
| author_facet | Rami Alkhatib Razan Hawamdeh Laith Al-Eitan Nour Abdo Fadi Obeidat Mohamed Al-Bataineh Hatem Aman |
| author_sort | Rami Alkhatib |
| collection | DOAJ |
| description | Abstract Background This study aimed to investigate the genetic association of specific Single Nucleotide Polymorphisms (SNPs) within the muscle segment homeobox gene 1 (MSX1) with susceptibility to the peg-shaped teeth in 36 Jordanian Arab families and case–control samples in the Jordanian Arab population. Methods This cohort involved 108 individuals (36 trios families), which were used for family-based genetic study. Additionally, 56 patients and 57 controls were used for case–control study. Genomic DNA samples from both families and case–control were extracted according to distinguished processes. Then, polymerase chain reaction technique (PCR) was conducted using specific primers for the axons of the MSX1. Moreover, DNA sequencing genotyping method analysis of SNPs was used to detect specified SNPs in the MSX1 linked with peg-shaped teeth. Hardy–Weinberg Equilibrium and Chi-square were used to evaluate the data quality and the presence of any genotypic error. In addition, Transmission Disequilibrium Test (TDT) was used identify family-based association in which trios of parents and proband are used. Results The results of this study showed fourteen polymorphic sites in this gene, eight of them (rs121913129, rs104893852, rs104893853, rs121913130, rs104893850, rs1095, rs3775261, and rs1042484) were none-polymorphic. Meanwhile, the minor allele frequencies of the rest of the SNPs were polymorphic (rs8670, rs12532, rs3821949, rs4464513, rs1907998, and rs6446693). However, none of these SNPs were associated with peg-shaped teeth. Moreover, the haplotype genetic analysis revealed that there was no genetic association with peg-shaped teeth disorder susceptibility (P > 0.05) in the Jordanian families of Arab descent. Conclusions The present findings can be used in estimation of prevalence of peg-shaped teeth in the Jordanian population. However, our findings revealed that there is no evidence that the MSX1 polymorphisms had a crucial role in the peg-shaped teeth phenomenon, emphasizing that other genes might have this role. These findings are beneficial for clinicians to comprehensively understand the molecular aspects of teeth abnormalities. |
| first_indexed | 2024-12-20T10:26:10Z |
| format | Article |
| id | doaj.art-54ac7605387b489b92df312b57b2b44c |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-12-20T10:26:10Z |
| publishDate | 2022-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-54ac7605387b489b92df312b57b2b44c2022-12-21T19:43:49ZengBMCBMC Oral Health1472-68312022-01-012211810.1186/s12903-022-02051-2Family and case–control genetic study of MSX1 polymorphisms in peg-shaped teeth Jordanian populationRami Alkhatib0Razan Hawamdeh1Laith Al-Eitan2Nour Abdo3Fadi Obeidat4Mohamed Al-Bataineh5Hatem Aman6Department of Applied Biological Sciences, Jordan University of Science and TechnologyDepartment of Applied Biological Sciences, Jordan University of Science and TechnologyDepartment of Applied Biological Sciences, Jordan University of Science and TechnologyDepartment of Public Health, Faculty of Medicine, Jordan University of Science and TechnologyDepartment of Dentistry, Jordanian Royal Medical ServicesDepartment of Dentistry, Jordanian Royal Medical ServicesDepartment of Biotechnology and Genetic Engineering, Jordan University of Science and TechnologyAbstract Background This study aimed to investigate the genetic association of specific Single Nucleotide Polymorphisms (SNPs) within the muscle segment homeobox gene 1 (MSX1) with susceptibility to the peg-shaped teeth in 36 Jordanian Arab families and case–control samples in the Jordanian Arab population. Methods This cohort involved 108 individuals (36 trios families), which were used for family-based genetic study. Additionally, 56 patients and 57 controls were used for case–control study. Genomic DNA samples from both families and case–control were extracted according to distinguished processes. Then, polymerase chain reaction technique (PCR) was conducted using specific primers for the axons of the MSX1. Moreover, DNA sequencing genotyping method analysis of SNPs was used to detect specified SNPs in the MSX1 linked with peg-shaped teeth. Hardy–Weinberg Equilibrium and Chi-square were used to evaluate the data quality and the presence of any genotypic error. In addition, Transmission Disequilibrium Test (TDT) was used identify family-based association in which trios of parents and proband are used. Results The results of this study showed fourteen polymorphic sites in this gene, eight of them (rs121913129, rs104893852, rs104893853, rs121913130, rs104893850, rs1095, rs3775261, and rs1042484) were none-polymorphic. Meanwhile, the minor allele frequencies of the rest of the SNPs were polymorphic (rs8670, rs12532, rs3821949, rs4464513, rs1907998, and rs6446693). However, none of these SNPs were associated with peg-shaped teeth. Moreover, the haplotype genetic analysis revealed that there was no genetic association with peg-shaped teeth disorder susceptibility (P > 0.05) in the Jordanian families of Arab descent. Conclusions The present findings can be used in estimation of prevalence of peg-shaped teeth in the Jordanian population. However, our findings revealed that there is no evidence that the MSX1 polymorphisms had a crucial role in the peg-shaped teeth phenomenon, emphasizing that other genes might have this role. These findings are beneficial for clinicians to comprehensively understand the molecular aspects of teeth abnormalities.https://doi.org/10.1186/s12903-022-02051-2MSX1Allele frequencySNPsExonsPrimers |
| spellingShingle | Rami Alkhatib Razan Hawamdeh Laith Al-Eitan Nour Abdo Fadi Obeidat Mohamed Al-Bataineh Hatem Aman Family and case–control genetic study of MSX1 polymorphisms in peg-shaped teeth Jordanian population BMC Oral Health MSX1 Allele frequency SNPs Exons Primers |
| title | Family and case–control genetic study of MSX1 polymorphisms in peg-shaped teeth Jordanian population |
| title_full | Family and case–control genetic study of MSX1 polymorphisms in peg-shaped teeth Jordanian population |
| title_fullStr | Family and case–control genetic study of MSX1 polymorphisms in peg-shaped teeth Jordanian population |
| title_full_unstemmed | Family and case–control genetic study of MSX1 polymorphisms in peg-shaped teeth Jordanian population |
| title_short | Family and case–control genetic study of MSX1 polymorphisms in peg-shaped teeth Jordanian population |
| title_sort | family and case control genetic study of msx1 polymorphisms in peg shaped teeth jordanian population |
| topic | MSX1 Allele frequency SNPs Exons Primers |
| url | https://doi.org/10.1186/s12903-022-02051-2 |
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