Rotation of Multiple Single-Gene Transgenic Crops Did Not Slow the Evolution of Resistance to Cry1F or Cry1Ie in <i>Ostrinia furnacalis</i>
A common strategy for delaying the evolution of resistance to transgenic crops that produce insecticidal proteins from <i>Bacillus thuringiensis</i> is to ensure that insect pests are exposed to multiple toxins with different mechanisms of action (MoAs). This can take the form of plantin...
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MDPI AG
2023-01-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/1/74 |
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| author | Yueqin Wang Yudong Quan Zhenying Wang Kanglai He |
| author_facet | Yueqin Wang Yudong Quan Zhenying Wang Kanglai He |
| author_sort | Yueqin Wang |
| collection | DOAJ |
| description | A common strategy for delaying the evolution of resistance to transgenic crops that produce insecticidal proteins from <i>Bacillus thuringiensis</i> is to ensure that insect pests are exposed to multiple toxins with different mechanisms of action (MoAs). This can take the form of planting crops in a rotation pattern when different crops expressing single toxins are available on the market. The efficacy of a rotation strategy is reliant on mathematical models based on biological assumptions. Here, we designed laboratory evolution experiments to test whether Bt-based insecticidal proteins with different MoAs used in rotation could delay resistance from developing in Asian corn borer (ACB), <i>Ostrinia furnacalis</i>. We investigated the proteins Cry1Ab, Cry1F, and Cry1Ie, which are widely utilized for commercial insect control. We found that rotation of multiple toxins did not slow the evolution of resistance to Cry1F or Cry1Ie. Furthermore, the evolution of ACB to the Cry1Ab toxin develops faster when Cry1F or Cry1Ie is present, as compared to Cry1Ab exposure only. Our results suggest that toxins used in a rotation fashion do not work as an effective strategy in delaying ACB resistance evolution to Cry toxins over one-toxin exposure. Our result highlights the need to better understand the biological factors leading to insecticidal protein resistance and to develop IRM strategies against target insects. |
| first_indexed | 2024-03-09T12:13:45Z |
| format | Article |
| id | doaj.art-54ae619d70ce41e088ce30ad2f30e521 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T12:13:45Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-54ae619d70ce41e088ce30ad2f30e5212023-11-30T22:48:38ZengMDPI AGInsects2075-44502023-01-011417410.3390/insects14010074Rotation of Multiple Single-Gene Transgenic Crops Did Not Slow the Evolution of Resistance to Cry1F or Cry1Ie in <i>Ostrinia furnacalis</i>Yueqin Wang0Yudong Quan1Zhenying Wang2Kanglai He3State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaA common strategy for delaying the evolution of resistance to transgenic crops that produce insecticidal proteins from <i>Bacillus thuringiensis</i> is to ensure that insect pests are exposed to multiple toxins with different mechanisms of action (MoAs). This can take the form of planting crops in a rotation pattern when different crops expressing single toxins are available on the market. The efficacy of a rotation strategy is reliant on mathematical models based on biological assumptions. Here, we designed laboratory evolution experiments to test whether Bt-based insecticidal proteins with different MoAs used in rotation could delay resistance from developing in Asian corn borer (ACB), <i>Ostrinia furnacalis</i>. We investigated the proteins Cry1Ab, Cry1F, and Cry1Ie, which are widely utilized for commercial insect control. We found that rotation of multiple toxins did not slow the evolution of resistance to Cry1F or Cry1Ie. Furthermore, the evolution of ACB to the Cry1Ab toxin develops faster when Cry1F or Cry1Ie is present, as compared to Cry1Ab exposure only. Our results suggest that toxins used in a rotation fashion do not work as an effective strategy in delaying ACB resistance evolution to Cry toxins over one-toxin exposure. Our result highlights the need to better understand the biological factors leading to insecticidal protein resistance and to develop IRM strategies against target insects.https://www.mdpi.com/2075-4450/14/1/74<i>Bacillus thuringiensis</i>resistancerotation<i>Ostrinia furnacalis</i>resistance management |
| spellingShingle | Yueqin Wang Yudong Quan Zhenying Wang Kanglai He Rotation of Multiple Single-Gene Transgenic Crops Did Not Slow the Evolution of Resistance to Cry1F or Cry1Ie in <i>Ostrinia furnacalis</i> Insects <i>Bacillus thuringiensis</i> resistance rotation <i>Ostrinia furnacalis</i> resistance management |
| title | Rotation of Multiple Single-Gene Transgenic Crops Did Not Slow the Evolution of Resistance to Cry1F or Cry1Ie in <i>Ostrinia furnacalis</i> |
| title_full | Rotation of Multiple Single-Gene Transgenic Crops Did Not Slow the Evolution of Resistance to Cry1F or Cry1Ie in <i>Ostrinia furnacalis</i> |
| title_fullStr | Rotation of Multiple Single-Gene Transgenic Crops Did Not Slow the Evolution of Resistance to Cry1F or Cry1Ie in <i>Ostrinia furnacalis</i> |
| title_full_unstemmed | Rotation of Multiple Single-Gene Transgenic Crops Did Not Slow the Evolution of Resistance to Cry1F or Cry1Ie in <i>Ostrinia furnacalis</i> |
| title_short | Rotation of Multiple Single-Gene Transgenic Crops Did Not Slow the Evolution of Resistance to Cry1F or Cry1Ie in <i>Ostrinia furnacalis</i> |
| title_sort | rotation of multiple single gene transgenic crops did not slow the evolution of resistance to cry1f or cry1ie in i ostrinia furnacalis i |
| topic | <i>Bacillus thuringiensis</i> resistance rotation <i>Ostrinia furnacalis</i> resistance management |
| url | https://www.mdpi.com/2075-4450/14/1/74 |
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