Metabolomics Analysis Reveals Metabolites and Metabolic Pathways Involved in the Growth and Quality of Water Dropwort [<i>Oenanthe javanica</i> (Blume) DC.] under Nutrient Solution Culture
Water dropwort (<i>Oenanthe javanica</i> (Blume) DC.) is an important vegetable crop. Nutrient liquid culture has become an important cultivation method in the production of water dropwort. However, the effects of different nutrient solution cultivation methods on the growth and quality...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/12/7/1459 |
| _version_ | 1797607185459445760 |
|---|---|
| author | Kai Feng Xia-Yue Kan Qing Liu Ya-Jie Yan Nan Sun Zhi-Yuan Yang Shu-Ping Zhao Peng Wu Liang-Jun Li |
| author_facet | Kai Feng Xia-Yue Kan Qing Liu Ya-Jie Yan Nan Sun Zhi-Yuan Yang Shu-Ping Zhao Peng Wu Liang-Jun Li |
| author_sort | Kai Feng |
| collection | DOAJ |
| description | Water dropwort (<i>Oenanthe javanica</i> (Blume) DC.) is an important vegetable crop. Nutrient liquid culture has become an important cultivation method in the production of water dropwort. However, the effects of different nutrient solution cultivation methods on the growth and quality of water dropwort remains unclear. In this study, to screen the most suitable nutrient solution formula for the cultivation of water dropwort, the effects of different nutrient solution formulas (Hoagland, Cooper, Dutch greenhouse, Garden-style, Yamasaki and SCAU) on plant physiological and quality characteristics are investigated. The plant height, root length, water content (%), distribution rate of dry matter (%), chlorophyll, VC, flavonoid, total phenolic, DPPH and dietary fiber of water dropwort under different nutrient solutions were determined. According to the analytic hierarchy process (AHP) of the growth index and quality index of water dropwort under different nutrient solutions, the Yamazaki nutrient solution was considered to be the most suitable nutrient solution formula for water dropwort. To further confirm the differences of water dropwort under nutrient solution culture and soil culture, the broadly targeted metabolomics were performed. A total of 485 metabolites were detected in water dropwort under optimal nutrient solution and soil cultivation. Metabolomics analysis showed that flavonoids were the most abundant differential accumulated metabolites, and most flavonoids were up-regulated. A qRT-PCR assay indicated that the structural genes of the flavonoid biosynthesis pathway (<i>PAL, C4H, CHS, CHI, F3H, DFR, UFGT</i>) were significantly higher under the Yamasaki nutrient solution treatment. The current study provided a theoretical basis and technical guidance for the nutrient solution cultivation of water dropwort. Meanwhile, this study provides new insights into the study of flavonoids in water dropwort. |
| first_indexed | 2024-03-11T05:27:39Z |
| format | Article |
| id | doaj.art-54b22f96d4cc476c9867a932399b2428 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T05:27:39Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-54b22f96d4cc476c9867a932399b24282023-11-17T17:22:51ZengMDPI AGPlants2223-77472023-03-01127145910.3390/plants12071459Metabolomics Analysis Reveals Metabolites and Metabolic Pathways Involved in the Growth and Quality of Water Dropwort [<i>Oenanthe javanica</i> (Blume) DC.] under Nutrient Solution CultureKai Feng0Xia-Yue Kan1Qing Liu2Ya-Jie Yan3Nan Sun4Zhi-Yuan Yang5Shu-Ping Zhao6Peng Wu7Liang-Jun Li8College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaWater dropwort (<i>Oenanthe javanica</i> (Blume) DC.) is an important vegetable crop. Nutrient liquid culture has become an important cultivation method in the production of water dropwort. However, the effects of different nutrient solution cultivation methods on the growth and quality of water dropwort remains unclear. In this study, to screen the most suitable nutrient solution formula for the cultivation of water dropwort, the effects of different nutrient solution formulas (Hoagland, Cooper, Dutch greenhouse, Garden-style, Yamasaki and SCAU) on plant physiological and quality characteristics are investigated. The plant height, root length, water content (%), distribution rate of dry matter (%), chlorophyll, VC, flavonoid, total phenolic, DPPH and dietary fiber of water dropwort under different nutrient solutions were determined. According to the analytic hierarchy process (AHP) of the growth index and quality index of water dropwort under different nutrient solutions, the Yamazaki nutrient solution was considered to be the most suitable nutrient solution formula for water dropwort. To further confirm the differences of water dropwort under nutrient solution culture and soil culture, the broadly targeted metabolomics were performed. A total of 485 metabolites were detected in water dropwort under optimal nutrient solution and soil cultivation. Metabolomics analysis showed that flavonoids were the most abundant differential accumulated metabolites, and most flavonoids were up-regulated. A qRT-PCR assay indicated that the structural genes of the flavonoid biosynthesis pathway (<i>PAL, C4H, CHS, CHI, F3H, DFR, UFGT</i>) were significantly higher under the Yamasaki nutrient solution treatment. The current study provided a theoretical basis and technical guidance for the nutrient solution cultivation of water dropwort. Meanwhile, this study provides new insights into the study of flavonoids in water dropwort.https://www.mdpi.com/2223-7747/12/7/1459<i>Oenanthe javanica</i> (Blume) DC.gene expressionnutrient solution culturemetabolites |
| spellingShingle | Kai Feng Xia-Yue Kan Qing Liu Ya-Jie Yan Nan Sun Zhi-Yuan Yang Shu-Ping Zhao Peng Wu Liang-Jun Li Metabolomics Analysis Reveals Metabolites and Metabolic Pathways Involved in the Growth and Quality of Water Dropwort [<i>Oenanthe javanica</i> (Blume) DC.] under Nutrient Solution Culture Plants <i>Oenanthe javanica</i> (Blume) DC. gene expression nutrient solution culture metabolites |
| title | Metabolomics Analysis Reveals Metabolites and Metabolic Pathways Involved in the Growth and Quality of Water Dropwort [<i>Oenanthe javanica</i> (Blume) DC.] under Nutrient Solution Culture |
| title_full | Metabolomics Analysis Reveals Metabolites and Metabolic Pathways Involved in the Growth and Quality of Water Dropwort [<i>Oenanthe javanica</i> (Blume) DC.] under Nutrient Solution Culture |
| title_fullStr | Metabolomics Analysis Reveals Metabolites and Metabolic Pathways Involved in the Growth and Quality of Water Dropwort [<i>Oenanthe javanica</i> (Blume) DC.] under Nutrient Solution Culture |
| title_full_unstemmed | Metabolomics Analysis Reveals Metabolites and Metabolic Pathways Involved in the Growth and Quality of Water Dropwort [<i>Oenanthe javanica</i> (Blume) DC.] under Nutrient Solution Culture |
| title_short | Metabolomics Analysis Reveals Metabolites and Metabolic Pathways Involved in the Growth and Quality of Water Dropwort [<i>Oenanthe javanica</i> (Blume) DC.] under Nutrient Solution Culture |
| title_sort | metabolomics analysis reveals metabolites and metabolic pathways involved in the growth and quality of water dropwort i oenanthe javanica i blume dc under nutrient solution culture |
| topic | <i>Oenanthe javanica</i> (Blume) DC. gene expression nutrient solution culture metabolites |
| url | https://www.mdpi.com/2223-7747/12/7/1459 |
| work_keys_str_mv | AT kaifeng metabolomicsanalysisrevealsmetabolitesandmetabolicpathwaysinvolvedinthegrowthandqualityofwaterdropwortioenanthejavanicaiblumedcundernutrientsolutionculture AT xiayuekan metabolomicsanalysisrevealsmetabolitesandmetabolicpathwaysinvolvedinthegrowthandqualityofwaterdropwortioenanthejavanicaiblumedcundernutrientsolutionculture AT qingliu metabolomicsanalysisrevealsmetabolitesandmetabolicpathwaysinvolvedinthegrowthandqualityofwaterdropwortioenanthejavanicaiblumedcundernutrientsolutionculture AT yajieyan metabolomicsanalysisrevealsmetabolitesandmetabolicpathwaysinvolvedinthegrowthandqualityofwaterdropwortioenanthejavanicaiblumedcundernutrientsolutionculture AT nansun metabolomicsanalysisrevealsmetabolitesandmetabolicpathwaysinvolvedinthegrowthandqualityofwaterdropwortioenanthejavanicaiblumedcundernutrientsolutionculture AT zhiyuanyang metabolomicsanalysisrevealsmetabolitesandmetabolicpathwaysinvolvedinthegrowthandqualityofwaterdropwortioenanthejavanicaiblumedcundernutrientsolutionculture AT shupingzhao metabolomicsanalysisrevealsmetabolitesandmetabolicpathwaysinvolvedinthegrowthandqualityofwaterdropwortioenanthejavanicaiblumedcundernutrientsolutionculture AT pengwu metabolomicsanalysisrevealsmetabolitesandmetabolicpathwaysinvolvedinthegrowthandqualityofwaterdropwortioenanthejavanicaiblumedcundernutrientsolutionculture AT liangjunli metabolomicsanalysisrevealsmetabolitesandmetabolicpathwaysinvolvedinthegrowthandqualityofwaterdropwortioenanthejavanicaiblumedcundernutrientsolutionculture |