Evaluation of the Conditions for the Cultivation of Callus Cultures of <i>Hyssopus officinalis</i> Regarding the Yield of Polyphenolic Compounds
The cultivation of plants in the form of callus cultures constitutes a renewable source of secondary plant metabolites. The conditions for the cultivation of callus cultures affect the yield of target compounds. Callus cultures of <i>Hyssopus officinalis</i> were chosen for study. Nutrie...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/5/915 |
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| author | Olga Babich Stanislav Sukhikh Artem Pungin Lidiya Astahova Evgeny Chupakhin Daria Belova Alexander Prosekov Svetlana Ivanova |
| author_facet | Olga Babich Stanislav Sukhikh Artem Pungin Lidiya Astahova Evgeny Chupakhin Daria Belova Alexander Prosekov Svetlana Ivanova |
| author_sort | Olga Babich |
| collection | DOAJ |
| description | The cultivation of plants in the form of callus cultures constitutes a renewable source of secondary plant metabolites. The conditions for the cultivation of callus cultures affect the yield of target compounds. Callus cultures of <i>Hyssopus officinalis</i> were chosen for study. Nutrient media of various compositions were used for <i>Hyssopus officinalis</i> callus culture. For each culture, data on the quantitative contents of saponins, flavonoids and polyphenolic compounds, as well as antioxidant activity, were obtained. It was found that Murashige and Skoog medium supplemented with 1-naphthylacetic acid and kinetin led to the highest yield of secondary metabolites. |
| first_indexed | 2024-03-10T11:44:13Z |
| format | Article |
| id | doaj.art-54b692f455c84ffabb0bbe628ebab969 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T11:44:13Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-54b692f455c84ffabb0bbe628ebab9692023-11-21T18:13:11ZengMDPI AGPlants2223-77472021-05-0110591510.3390/plants10050915Evaluation of the Conditions for the Cultivation of Callus Cultures of <i>Hyssopus officinalis</i> Regarding the Yield of Polyphenolic CompoundsOlga Babich0Stanislav Sukhikh1Artem Pungin2Lidiya Astahova3Evgeny Chupakhin4Daria Belova5Alexander Prosekov6Svetlana Ivanova7Institute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, RussiaInstitute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, RussiaInstitute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, RussiaInstitute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, RussiaInstitute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, RussiaInstitute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, RussiaLaboratory of Biocatalysis, Kemerovo State University, Krasnaya Street 6, 650043 Kemerovo, RussiaNatural Nutraceutical Biotesting Laboratory, Kemerovo State University, Krasnaya Street 6, 650043 Kemerovo, RussiaThe cultivation of plants in the form of callus cultures constitutes a renewable source of secondary plant metabolites. The conditions for the cultivation of callus cultures affect the yield of target compounds. Callus cultures of <i>Hyssopus officinalis</i> were chosen for study. Nutrient media of various compositions were used for <i>Hyssopus officinalis</i> callus culture. For each culture, data on the quantitative contents of saponins, flavonoids and polyphenolic compounds, as well as antioxidant activity, were obtained. It was found that Murashige and Skoog medium supplemented with 1-naphthylacetic acid and kinetin led to the highest yield of secondary metabolites.https://www.mdpi.com/2223-7747/10/5/915callus culturesmedicinal hyssop (<i>Hyssopus officinalis</i> L.)medicinal plantsbiologically active substances |
| spellingShingle | Olga Babich Stanislav Sukhikh Artem Pungin Lidiya Astahova Evgeny Chupakhin Daria Belova Alexander Prosekov Svetlana Ivanova Evaluation of the Conditions for the Cultivation of Callus Cultures of <i>Hyssopus officinalis</i> Regarding the Yield of Polyphenolic Compounds Plants callus cultures medicinal hyssop (<i>Hyssopus officinalis</i> L.) medicinal plants biologically active substances |
| title | Evaluation of the Conditions for the Cultivation of Callus Cultures of <i>Hyssopus officinalis</i> Regarding the Yield of Polyphenolic Compounds |
| title_full | Evaluation of the Conditions for the Cultivation of Callus Cultures of <i>Hyssopus officinalis</i> Regarding the Yield of Polyphenolic Compounds |
| title_fullStr | Evaluation of the Conditions for the Cultivation of Callus Cultures of <i>Hyssopus officinalis</i> Regarding the Yield of Polyphenolic Compounds |
| title_full_unstemmed | Evaluation of the Conditions for the Cultivation of Callus Cultures of <i>Hyssopus officinalis</i> Regarding the Yield of Polyphenolic Compounds |
| title_short | Evaluation of the Conditions for the Cultivation of Callus Cultures of <i>Hyssopus officinalis</i> Regarding the Yield of Polyphenolic Compounds |
| title_sort | evaluation of the conditions for the cultivation of callus cultures of i hyssopus officinalis i regarding the yield of polyphenolic compounds |
| topic | callus cultures medicinal hyssop (<i>Hyssopus officinalis</i> L.) medicinal plants biologically active substances |
| url | https://www.mdpi.com/2223-7747/10/5/915 |
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