Comparative efficacy of bio-selenium nanoparticles and sodium selenite on morpho-physiochemical attributes under normal and salt stress conditions, besides selenium detoxification pathways in Brassica napus L.
Abstract Selenium nanoparticles (SeNPs) have attracted considerable attention globally due to their significant potential for alleviating abiotic stresses in plants. Accordingly, further research has been conducted to develop nanoparticles using chemical ways. However, our knowledge about the potent...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-03-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-022-01370-4 |
| _version_ | 1811264200402534400 |
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| author | Ali Mahmoud El-Badri Ahmed M. Hashem Maria Batool Ahmed Sherif Elsayed Nishawy Mohammed Ayaad Hamada M. Hassan Ibrahim M. Elrewainy Jing Wang Jie Kuai Bo Wang Shixue Zheng Guangsheng Zhou |
| author_facet | Ali Mahmoud El-Badri Ahmed M. Hashem Maria Batool Ahmed Sherif Elsayed Nishawy Mohammed Ayaad Hamada M. Hassan Ibrahim M. Elrewainy Jing Wang Jie Kuai Bo Wang Shixue Zheng Guangsheng Zhou |
| author_sort | Ali Mahmoud El-Badri |
| collection | DOAJ |
| description | Abstract Selenium nanoparticles (SeNPs) have attracted considerable attention globally due to their significant potential for alleviating abiotic stresses in plants. Accordingly, further research has been conducted to develop nanoparticles using chemical ways. However, our knowledge about the potential benefit or phytotoxicity of bioSeNPs in rapeseed is still unclear. Herein, we investigated the effect of bioSeNPs on growth and physiochemical attributes, and selenium detoxification pathways compared to sodium selenite (Se (IV)) during the early seedling stage under normal and salt stress conditions. Our findings showed that the range between optimal and toxic levels of bioSeNPs was wider than Se (IV), which increased the plant’s ability to reduce salinity-induced oxidative stress. BioSeNPs improved the phenotypic characteristics of rapeseed seedlings without the sign of toxicity, markedly elevated germination, growth, photosynthetic efficiency and osmolyte accumulation versus Se (IV) under normal and salt stress conditions. In addition to modulation of Na+ and K+ uptake, bioSeNPs minimized the ROS level and MDA content by activating the antioxidant enzymes engaged in ROS detoxification by regulating these enzyme-related genes expression patterns. Importantly, the main effect of bioSeNPs and Se (IV) on plant growth appeared to be correlated with the change in the expression levels of Se-related genes. Our qRT-PCR results revealed that the genes involved in Se detoxification in root tissue were upregulated upon Se (IV) treated seedlings compared to NPs, indicating that bioSeNPs have a slightly toxic effect under higher concentrations. Furthermore, bioSeNPs might improve lateral root production by increasing the expression level of LBD16. Taken together, transamination and selenation were more functional methods of Se detoxification and proposed different degradation pathways that synthesized malformed or deformed selenoproteins, which provided essential mechanisms to increase Se tolerance at higher concentrations in rapeseed seedlings. Current findings could add more knowledge regarding the mechanisms underlying bioSeNPs induced plant growth. Graphical Abstract |
| first_indexed | 2024-04-12T19:58:52Z |
| format | Article |
| id | doaj.art-54b9a25777b2492fa0fda726fac7d377 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-12T19:58:52Z |
| publishDate | 2022-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-54b9a25777b2492fa0fda726fac7d3772022-12-22T03:18:35ZengBMCJournal of Nanobiotechnology1477-31552022-03-0120112310.1186/s12951-022-01370-4Comparative efficacy of bio-selenium nanoparticles and sodium selenite on morpho-physiochemical attributes under normal and salt stress conditions, besides selenium detoxification pathways in Brassica napus L.Ali Mahmoud El-Badri0Ahmed M. Hashem1Maria Batool2Ahmed Sherif3Elsayed Nishawy4Mohammed Ayaad5Hamada M. Hassan6Ibrahim M. Elrewainy7Jing Wang8Jie Kuai9Bo Wang10Shixue Zheng11Guangsheng Zhou12MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural UniversityMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural UniversityMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural UniversityMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural UniversityDesert Research Center, Genetics Resource Department, Egyptian Deserts Gene BankPlant Research Department, Nuclear Research Center, Atomic Energy AuthorityField Crops Research Institute, Agricultural Research Center (ARC)Field Crops Research Institute, Agricultural Research Center (ARC)MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural UniversityMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural UniversityMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural UniversityState Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural UniversityMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural UniversityAbstract Selenium nanoparticles (SeNPs) have attracted considerable attention globally due to their significant potential for alleviating abiotic stresses in plants. Accordingly, further research has been conducted to develop nanoparticles using chemical ways. However, our knowledge about the potential benefit or phytotoxicity of bioSeNPs in rapeseed is still unclear. Herein, we investigated the effect of bioSeNPs on growth and physiochemical attributes, and selenium detoxification pathways compared to sodium selenite (Se (IV)) during the early seedling stage under normal and salt stress conditions. Our findings showed that the range between optimal and toxic levels of bioSeNPs was wider than Se (IV), which increased the plant’s ability to reduce salinity-induced oxidative stress. BioSeNPs improved the phenotypic characteristics of rapeseed seedlings without the sign of toxicity, markedly elevated germination, growth, photosynthetic efficiency and osmolyte accumulation versus Se (IV) under normal and salt stress conditions. In addition to modulation of Na+ and K+ uptake, bioSeNPs minimized the ROS level and MDA content by activating the antioxidant enzymes engaged in ROS detoxification by regulating these enzyme-related genes expression patterns. Importantly, the main effect of bioSeNPs and Se (IV) on plant growth appeared to be correlated with the change in the expression levels of Se-related genes. Our qRT-PCR results revealed that the genes involved in Se detoxification in root tissue were upregulated upon Se (IV) treated seedlings compared to NPs, indicating that bioSeNPs have a slightly toxic effect under higher concentrations. Furthermore, bioSeNPs might improve lateral root production by increasing the expression level of LBD16. Taken together, transamination and selenation were more functional methods of Se detoxification and proposed different degradation pathways that synthesized malformed or deformed selenoproteins, which provided essential mechanisms to increase Se tolerance at higher concentrations in rapeseed seedlings. Current findings could add more knowledge regarding the mechanisms underlying bioSeNPs induced plant growth. Graphical Abstracthttps://doi.org/10.1186/s12951-022-01370-4Bio-selenium nanoparticlesSodium seleniteSelenium detoxificationSalt stressBrassica napus L. |
| spellingShingle | Ali Mahmoud El-Badri Ahmed M. Hashem Maria Batool Ahmed Sherif Elsayed Nishawy Mohammed Ayaad Hamada M. Hassan Ibrahim M. Elrewainy Jing Wang Jie Kuai Bo Wang Shixue Zheng Guangsheng Zhou Comparative efficacy of bio-selenium nanoparticles and sodium selenite on morpho-physiochemical attributes under normal and salt stress conditions, besides selenium detoxification pathways in Brassica napus L. Journal of Nanobiotechnology Bio-selenium nanoparticles Sodium selenite Selenium detoxification Salt stress Brassica napus L. |
| title | Comparative efficacy of bio-selenium nanoparticles and sodium selenite on morpho-physiochemical attributes under normal and salt stress conditions, besides selenium detoxification pathways in Brassica napus L. |
| title_full | Comparative efficacy of bio-selenium nanoparticles and sodium selenite on morpho-physiochemical attributes under normal and salt stress conditions, besides selenium detoxification pathways in Brassica napus L. |
| title_fullStr | Comparative efficacy of bio-selenium nanoparticles and sodium selenite on morpho-physiochemical attributes under normal and salt stress conditions, besides selenium detoxification pathways in Brassica napus L. |
| title_full_unstemmed | Comparative efficacy of bio-selenium nanoparticles and sodium selenite on morpho-physiochemical attributes under normal and salt stress conditions, besides selenium detoxification pathways in Brassica napus L. |
| title_short | Comparative efficacy of bio-selenium nanoparticles and sodium selenite on morpho-physiochemical attributes under normal and salt stress conditions, besides selenium detoxification pathways in Brassica napus L. |
| title_sort | comparative efficacy of bio selenium nanoparticles and sodium selenite on morpho physiochemical attributes under normal and salt stress conditions besides selenium detoxification pathways in brassica napus l |
| topic | Bio-selenium nanoparticles Sodium selenite Selenium detoxification Salt stress Brassica napus L. |
| url | https://doi.org/10.1186/s12951-022-01370-4 |
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