Isolation, Identification, and Complete Genome Assembly of an Endophytic Bacillus velezensis YB-130, Potential Biocontrol Agent Against Fusarium graminearum
Wheat scab caused by F. graminearum is a highly destructive disease that leads to yield reduction and mycotoxin contamination of grains. In this study, an endophytic bacterium of strain YB-130 was isolated from surface sterilized wheat spikes with scab symptoms and identified as Bacillus velezensis...
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Frontiers Media S.A.
2020-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2020.598285/full |
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| author | Wen Xu Liyong Zhang Liyong Zhang Paul H. Goodwin Mingcong Xia Jie Zhang Qi Wang Juan Liang Runhong Sun Chao Wu Lirong Yang |
| author_facet | Wen Xu Liyong Zhang Liyong Zhang Paul H. Goodwin Mingcong Xia Jie Zhang Qi Wang Juan Liang Runhong Sun Chao Wu Lirong Yang |
| author_sort | Wen Xu |
| collection | DOAJ |
| description | Wheat scab caused by F. graminearum is a highly destructive disease that leads to yield reduction and mycotoxin contamination of grains. In this study, an endophytic bacterium of strain YB-130 was isolated from surface sterilized wheat spikes with scab symptoms and identified as Bacillus velezensis by whole genome annotation, 16S rRNA gene and average nucleotide identities analysis. The whole-genome sequence of strain YB-130 was obtained by PacBio sequencing. 88 putative Carbohydrate-Active Enzymes and 12 gene clusters encoding for secondary metabolites were identified in the YB-130 genome, including one gene cluster for the synthesis of lanthipeptide only found in strain YB-130 genome. In dual cultures, strain YB-130 significantly inhibited the growth of F. graminearum PH-1 and other eight fungal plant pathogens, indicating a broad antifungal activity. Furthermore, strain YB-130 was able to significantly inhibit spore morphology and hyphal development of F. graminearum PH-1. Strain YB-130 also reduced deoxynivalenol production by F. graminearum PH-1 in dual cultures, possibly due to its ability to suppress the expression of tri5, tri3, and tri8 that are required for deoxynivalenol production in F. graminearum. Overall, B. velezensis YB-130 is a promising biological control agent of both F. graminearum infection and mycotoxin production. |
| first_indexed | 2024-12-17T01:12:49Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-17T01:12:49Z |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-54ba3823fa854cc790f4a3ae6231dd892022-12-21T22:09:06ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-12-011110.3389/fmicb.2020.598285598285Isolation, Identification, and Complete Genome Assembly of an Endophytic Bacillus velezensis YB-130, Potential Biocontrol Agent Against Fusarium graminearumWen Xu0Liyong Zhang1Liyong Zhang2Paul H. Goodwin3Mingcong Xia4Jie Zhang5Qi Wang6Juan Liang7Runhong Sun8Chao Wu9Lirong Yang10Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Biopesticide Engineering Research Center, Henan International Joint Laboratory of Crop Protection, Zhengzhou, ChinaInstitute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Biopesticide Engineering Research Center, Henan International Joint Laboratory of Crop Protection, Zhengzhou, ChinaCollege of Life Sciences, Henan Agricultural University, Zhengzhou, ChinaSchool of Environmental Sciences, University of Guelph, Guelph, ON, CanadaInstitute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Biopesticide Engineering Research Center, Henan International Joint Laboratory of Crop Protection, Zhengzhou, ChinaInstitute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Biopesticide Engineering Research Center, Henan International Joint Laboratory of Crop Protection, Zhengzhou, ChinaCollege of Plant Protection, China Agricultural University, Beijing, ChinaInstitute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Biopesticide Engineering Research Center, Henan International Joint Laboratory of Crop Protection, Zhengzhou, ChinaInstitute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Biopesticide Engineering Research Center, Henan International Joint Laboratory of Crop Protection, Zhengzhou, ChinaInstitute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Biopesticide Engineering Research Center, Henan International Joint Laboratory of Crop Protection, Zhengzhou, ChinaInstitute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Biopesticide Engineering Research Center, Henan International Joint Laboratory of Crop Protection, Zhengzhou, ChinaWheat scab caused by F. graminearum is a highly destructive disease that leads to yield reduction and mycotoxin contamination of grains. In this study, an endophytic bacterium of strain YB-130 was isolated from surface sterilized wheat spikes with scab symptoms and identified as Bacillus velezensis by whole genome annotation, 16S rRNA gene and average nucleotide identities analysis. The whole-genome sequence of strain YB-130 was obtained by PacBio sequencing. 88 putative Carbohydrate-Active Enzymes and 12 gene clusters encoding for secondary metabolites were identified in the YB-130 genome, including one gene cluster for the synthesis of lanthipeptide only found in strain YB-130 genome. In dual cultures, strain YB-130 significantly inhibited the growth of F. graminearum PH-1 and other eight fungal plant pathogens, indicating a broad antifungal activity. Furthermore, strain YB-130 was able to significantly inhibit spore morphology and hyphal development of F. graminearum PH-1. Strain YB-130 also reduced deoxynivalenol production by F. graminearum PH-1 in dual cultures, possibly due to its ability to suppress the expression of tri5, tri3, and tri8 that are required for deoxynivalenol production in F. graminearum. Overall, B. velezensis YB-130 is a promising biological control agent of both F. graminearum infection and mycotoxin production.https://www.frontiersin.org/articles/10.3389/fmicb.2020.598285/fullBacillus velezensisFusarium graminearumDONbiocontrol agentgenome sequencing and assembly |
| spellingShingle | Wen Xu Liyong Zhang Liyong Zhang Paul H. Goodwin Mingcong Xia Jie Zhang Qi Wang Juan Liang Runhong Sun Chao Wu Lirong Yang Isolation, Identification, and Complete Genome Assembly of an Endophytic Bacillus velezensis YB-130, Potential Biocontrol Agent Against Fusarium graminearum Frontiers in Microbiology Bacillus velezensis Fusarium graminearum DON biocontrol agent genome sequencing and assembly |
| title | Isolation, Identification, and Complete Genome Assembly of an Endophytic Bacillus velezensis YB-130, Potential Biocontrol Agent Against Fusarium graminearum |
| title_full | Isolation, Identification, and Complete Genome Assembly of an Endophytic Bacillus velezensis YB-130, Potential Biocontrol Agent Against Fusarium graminearum |
| title_fullStr | Isolation, Identification, and Complete Genome Assembly of an Endophytic Bacillus velezensis YB-130, Potential Biocontrol Agent Against Fusarium graminearum |
| title_full_unstemmed | Isolation, Identification, and Complete Genome Assembly of an Endophytic Bacillus velezensis YB-130, Potential Biocontrol Agent Against Fusarium graminearum |
| title_short | Isolation, Identification, and Complete Genome Assembly of an Endophytic Bacillus velezensis YB-130, Potential Biocontrol Agent Against Fusarium graminearum |
| title_sort | isolation identification and complete genome assembly of an endophytic bacillus velezensis yb 130 potential biocontrol agent against fusarium graminearum |
| topic | Bacillus velezensis Fusarium graminearum DON biocontrol agent genome sequencing and assembly |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2020.598285/full |
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