Comparison of Umbilical Cord Mesenchymal Stem Cells and Fibroblasts as Donor Nuclei for Handmade Cloning in Sheep Using a Single-Cell Transcriptome
Oocytes are efficient at reprogramming terminally differentiated cells to a totipotent state. Nuclear transfer techniques can exploit this property to produce cloned animals. However, the overall efficiency is low. The use of umbilical cord mesenchymal stem cells (UC-MSCs) as donor nuclei may increa...
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| Format: | Article |
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MDPI AG
2024-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/14/4/589 |
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| author | Weijian Li Yalan Liu Guizhen Zhou Zhuo Li Zhen Wang Li Wang Xiuling Ma Xuguang Wang |
| author_facet | Weijian Li Yalan Liu Guizhen Zhou Zhuo Li Zhen Wang Li Wang Xiuling Ma Xuguang Wang |
| author_sort | Weijian Li |
| collection | DOAJ |
| description | Oocytes are efficient at reprogramming terminally differentiated cells to a totipotent state. Nuclear transfer techniques can exploit this property to produce cloned animals. However, the overall efficiency is low. The use of umbilical cord mesenchymal stem cells (UC-MSCs) as donor nuclei may increase blastocyst rates, but the exact reasons for this remain unexplored. A single-cell transcriptomic approach was used to map the transcriptome profiles of eight-cell embryos that were in vitro-fertilized and handmade-cloned using umbilical cord mesenchymal stem cells and fibroblasts as nuclear donors. Differences were examined at the chromatin level, the level of differentially expressed genes, the level of histone modifications and the level of DNA methylation. This research provides critical information regarding the use of UC-MSCs as a preferred donor nucleus for nuclear transfer techniques. It also offers unique insights into the mechanism of cellular reprogramming. |
| first_indexed | 2024-03-07T22:45:26Z |
| format | Article |
| id | doaj.art-54bf13da5d38438ba575bbb9dea89266 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-07T22:45:26Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-54bf13da5d38438ba575bbb9dea892662024-02-23T15:04:45ZengMDPI AGAnimals2076-26152024-02-0114458910.3390/ani14040589Comparison of Umbilical Cord Mesenchymal Stem Cells and Fibroblasts as Donor Nuclei for Handmade Cloning in Sheep Using a Single-Cell TranscriptomeWeijian Li0Yalan Liu1Guizhen Zhou2Zhuo Li3Zhen Wang4Li Wang5Xiuling Ma6Xuguang Wang7College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Animal Science, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100091, ChinaCollege of Animal Science, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Animal Science, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Animal Science, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Animal Science, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Animal Science, Xinjiang Agricultural University, Urumqi 830052, ChinaOocytes are efficient at reprogramming terminally differentiated cells to a totipotent state. Nuclear transfer techniques can exploit this property to produce cloned animals. However, the overall efficiency is low. The use of umbilical cord mesenchymal stem cells (UC-MSCs) as donor nuclei may increase blastocyst rates, but the exact reasons for this remain unexplored. A single-cell transcriptomic approach was used to map the transcriptome profiles of eight-cell embryos that were in vitro-fertilized and handmade-cloned using umbilical cord mesenchymal stem cells and fibroblasts as nuclear donors. Differences were examined at the chromatin level, the level of differentially expressed genes, the level of histone modifications and the level of DNA methylation. This research provides critical information regarding the use of UC-MSCs as a preferred donor nucleus for nuclear transfer techniques. It also offers unique insights into the mechanism of cellular reprogramming.https://www.mdpi.com/2076-2615/14/4/589single-cell transcriptomeumbilical cord mesenchymal stem cellssheephandmade cloningnuclear transplantation |
| spellingShingle | Weijian Li Yalan Liu Guizhen Zhou Zhuo Li Zhen Wang Li Wang Xiuling Ma Xuguang Wang Comparison of Umbilical Cord Mesenchymal Stem Cells and Fibroblasts as Donor Nuclei for Handmade Cloning in Sheep Using a Single-Cell Transcriptome Animals single-cell transcriptome umbilical cord mesenchymal stem cells sheep handmade cloning nuclear transplantation |
| title | Comparison of Umbilical Cord Mesenchymal Stem Cells and Fibroblasts as Donor Nuclei for Handmade Cloning in Sheep Using a Single-Cell Transcriptome |
| title_full | Comparison of Umbilical Cord Mesenchymal Stem Cells and Fibroblasts as Donor Nuclei for Handmade Cloning in Sheep Using a Single-Cell Transcriptome |
| title_fullStr | Comparison of Umbilical Cord Mesenchymal Stem Cells and Fibroblasts as Donor Nuclei for Handmade Cloning in Sheep Using a Single-Cell Transcriptome |
| title_full_unstemmed | Comparison of Umbilical Cord Mesenchymal Stem Cells and Fibroblasts as Donor Nuclei for Handmade Cloning in Sheep Using a Single-Cell Transcriptome |
| title_short | Comparison of Umbilical Cord Mesenchymal Stem Cells and Fibroblasts as Donor Nuclei for Handmade Cloning in Sheep Using a Single-Cell Transcriptome |
| title_sort | comparison of umbilical cord mesenchymal stem cells and fibroblasts as donor nuclei for handmade cloning in sheep using a single cell transcriptome |
| topic | single-cell transcriptome umbilical cord mesenchymal stem cells sheep handmade cloning nuclear transplantation |
| url | https://www.mdpi.com/2076-2615/14/4/589 |
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