Virome Profiling, New Virus Identification and the Prevalence and Distribution of Viruses Infecting Chieh-Qua (<i>Benincasa hispida</i> Cogn. var. <i>chieh-qua</i> How) in China
The cucurbit vegetable chieh-qua (<i>Benincasa hispida</i> var. <i>chieh-qua</i> How) is an important crop in South China and southeast Asian countries. Viral diseases cause substantial loss of chieh-qua yield. To identify the viruses that affect chieh-qua in China, ribosomal...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/15/6/1396 |
| _version_ | 1797592355046424576 |
|---|---|
| author | Haiyan Che Yuxin Ma Yating Lin Tuizi Feng Daquan Luo Haibo Long |
| author_facet | Haiyan Che Yuxin Ma Yating Lin Tuizi Feng Daquan Luo Haibo Long |
| author_sort | Haiyan Che |
| collection | DOAJ |
| description | The cucurbit vegetable chieh-qua (<i>Benincasa hispida</i> var. <i>chieh-qua</i> How) is an important crop in South China and southeast Asian countries. Viral diseases cause substantial loss of chieh-qua yield. To identify the viruses that affect chieh-qua in China, ribosomal RNA-depleted total RNA sequencing was performed using chieh-qua leaf samples with typical viral symptoms. The virome of chieh-qua comprises four known viruses (melon yellow spot virus (MYSV), cucurbit chlorotic yellows virus (CCYV), papaya ringspot virus (PRSV) and watermelon silver mottle virus (WSMoV) and two novel viruses: cucurbit chlorotic virus (CuCV) in the genus <i>Crinivirus</i> and chieh-qua endornavirus (CqEV) in the genus <i>Alphaendornavirus</i>. The complete genomes of the two novel viruses in chieh-qua and three other isolates of CuCV in pumpkin, watermelon and cucumber were determined and the recombination signals of pumpkin and watermelon isolates of CuCV were detected. A reverse transcriptase PCR indicated that the dominant viruses of chieh-qua in Hainan are MYSV (66.67%) and CCYV (55.56%), followed by CuCV (27.41%), WSMoV (7.41%), cucumber mosaic virus (8.15%), zucchini yellow mosaic virus (6.67%), PRSV (6.67%) and CqEV (35.56%). Our findings support diagnostic and prevalence studies of viruses infecting chieh-qua in China, enabling sustainable control strategies for cucurbit viruses worldwide. |
| first_indexed | 2024-03-11T01:49:59Z |
| format | Article |
| id | doaj.art-54bf297c1ecc4e52bdb89a223d1a9489 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T01:49:59Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-54bf297c1ecc4e52bdb89a223d1a94892023-11-18T13:03:15ZengMDPI AGViruses1999-49152023-06-01156139610.3390/v15061396Virome Profiling, New Virus Identification and the Prevalence and Distribution of Viruses Infecting Chieh-Qua (<i>Benincasa hispida</i> Cogn. var. <i>chieh-qua</i> How) in ChinaHaiyan Che0Yuxin Ma1Yating Lin2Tuizi Feng3Daquan Luo4Haibo Long5Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaThe cucurbit vegetable chieh-qua (<i>Benincasa hispida</i> var. <i>chieh-qua</i> How) is an important crop in South China and southeast Asian countries. Viral diseases cause substantial loss of chieh-qua yield. To identify the viruses that affect chieh-qua in China, ribosomal RNA-depleted total RNA sequencing was performed using chieh-qua leaf samples with typical viral symptoms. The virome of chieh-qua comprises four known viruses (melon yellow spot virus (MYSV), cucurbit chlorotic yellows virus (CCYV), papaya ringspot virus (PRSV) and watermelon silver mottle virus (WSMoV) and two novel viruses: cucurbit chlorotic virus (CuCV) in the genus <i>Crinivirus</i> and chieh-qua endornavirus (CqEV) in the genus <i>Alphaendornavirus</i>. The complete genomes of the two novel viruses in chieh-qua and three other isolates of CuCV in pumpkin, watermelon and cucumber were determined and the recombination signals of pumpkin and watermelon isolates of CuCV were detected. A reverse transcriptase PCR indicated that the dominant viruses of chieh-qua in Hainan are MYSV (66.67%) and CCYV (55.56%), followed by CuCV (27.41%), WSMoV (7.41%), cucumber mosaic virus (8.15%), zucchini yellow mosaic virus (6.67%), PRSV (6.67%) and CqEV (35.56%). Our findings support diagnostic and prevalence studies of viruses infecting chieh-qua in China, enabling sustainable control strategies for cucurbit viruses worldwide.https://www.mdpi.com/1999-4915/15/6/1396chieh-quahigh-throughput sequencing<i>Orthotospovirus</i><i>Crinivirus</i><i>Potyvirus</i><i>Cucumovirus</i> |
| spellingShingle | Haiyan Che Yuxin Ma Yating Lin Tuizi Feng Daquan Luo Haibo Long Virome Profiling, New Virus Identification and the Prevalence and Distribution of Viruses Infecting Chieh-Qua (<i>Benincasa hispida</i> Cogn. var. <i>chieh-qua</i> How) in China Viruses chieh-qua high-throughput sequencing <i>Orthotospovirus</i> <i>Crinivirus</i> <i>Potyvirus</i> <i>Cucumovirus</i> |
| title | Virome Profiling, New Virus Identification and the Prevalence and Distribution of Viruses Infecting Chieh-Qua (<i>Benincasa hispida</i> Cogn. var. <i>chieh-qua</i> How) in China |
| title_full | Virome Profiling, New Virus Identification and the Prevalence and Distribution of Viruses Infecting Chieh-Qua (<i>Benincasa hispida</i> Cogn. var. <i>chieh-qua</i> How) in China |
| title_fullStr | Virome Profiling, New Virus Identification and the Prevalence and Distribution of Viruses Infecting Chieh-Qua (<i>Benincasa hispida</i> Cogn. var. <i>chieh-qua</i> How) in China |
| title_full_unstemmed | Virome Profiling, New Virus Identification and the Prevalence and Distribution of Viruses Infecting Chieh-Qua (<i>Benincasa hispida</i> Cogn. var. <i>chieh-qua</i> How) in China |
| title_short | Virome Profiling, New Virus Identification and the Prevalence and Distribution of Viruses Infecting Chieh-Qua (<i>Benincasa hispida</i> Cogn. var. <i>chieh-qua</i> How) in China |
| title_sort | virome profiling new virus identification and the prevalence and distribution of viruses infecting chieh qua i benincasa hispida i cogn var i chieh qua i how in china |
| topic | chieh-qua high-throughput sequencing <i>Orthotospovirus</i> <i>Crinivirus</i> <i>Potyvirus</i> <i>Cucumovirus</i> |
| url | https://www.mdpi.com/1999-4915/15/6/1396 |
| work_keys_str_mv | AT haiyanche viromeprofilingnewvirusidentificationandtheprevalenceanddistributionofvirusesinfectingchiehquaibenincasahispidaicognvarichiehquaihowinchina AT yuxinma viromeprofilingnewvirusidentificationandtheprevalenceanddistributionofvirusesinfectingchiehquaibenincasahispidaicognvarichiehquaihowinchina AT yatinglin viromeprofilingnewvirusidentificationandtheprevalenceanddistributionofvirusesinfectingchiehquaibenincasahispidaicognvarichiehquaihowinchina AT tuizifeng viromeprofilingnewvirusidentificationandtheprevalenceanddistributionofvirusesinfectingchiehquaibenincasahispidaicognvarichiehquaihowinchina AT daquanluo viromeprofilingnewvirusidentificationandtheprevalenceanddistributionofvirusesinfectingchiehquaibenincasahispidaicognvarichiehquaihowinchina AT haibolong viromeprofilingnewvirusidentificationandtheprevalenceanddistributionofvirusesinfectingchiehquaibenincasahispidaicognvarichiehquaihowinchina |